[1]杨蕊萍,钟 武.巨噬细胞极化相关差异基因的筛选及其在脓毒症中的潜在治疗价值[J].新乡医学院学报,2019,36(2):147-150.[doi:10.7683/xxyxyxb.2019.02.011]
 YANG Rui-ping,ZHONG Wu.Screening of differentially expressed genes related to macrophage polarization and its potential therapautic ralue in sepsis[J].Journal of Xinxiang Medical University,2019,36(2):147-150.[doi:10.7683/xxyxyxb.2019.02.011]
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巨噬细胞极化相关差异基因的筛选及其在脓毒症中的潜在治疗价值
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年2
页码:
147-150
栏目:
临床研究
出版日期:
2019-02-05

文章信息/Info

Title:
Screening of differentially expressed genes related to macrophage polarization and its potential therapautic ralue in sepsis
作者:
杨蕊萍钟 武
(西南医科大学附属医院急诊科,四川 泸州 646000)
Author(s):
YANG Rui-pingZHONG Wu
(Department of Emergency,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China)
关键词:
脓毒症巨噬细胞极化差异基因功能富集分析蛋白质交互作用
Keywords:
sepsismacrophage polarizationdifferentially expressed genesfunctional enrichment analysisprotein-protein interaction
分类号:
R631
DOI:
10.7683/xxyxyxb.2019.02.011
文献标志码:
A
摘要:
目的 运用生物信息学方法寻找巨噬细胞极化成M1、M2型的差异基因,为临床筛选脓毒症的免疫调节治疗靶点提供理论依据。方法 从综合性基因表达(GEO)数据库下载2套基因芯片数据,以在γ-干扰素(IFN-γ)及脂多糖(LPS)作用下极化为M1型巨噬细胞,白细胞介素-4(IL-4)作用下极化为M2型巨噬细胞为条件筛选样本,并提交至GEO2R平台进行在线分析,将筛选出的差异基因取交集作为最终的差异基因。利用DAVID软件对差异基因进行GO富集分析及KEGG信号通路分析,同时在STRING数据库行蛋白质交互作用分析。结果 共筛选出1 241个差异基因,其中在M1型巨噬细胞中表达上调而在M2型巨噬细胞中表达下调的基因有591个,在M1型巨噬细胞中表达下调而在M2型巨噬细胞中表达上调的基因有650个。功能富集分析结果显示,在M1型巨噬细胞中表达上调而在M2型巨噬细胞中表达下调的基因主要涉及炎症反应、细胞凋亡等功能,在M1型巨噬细胞中表达下调而在M2型巨噬细胞中表达上调的基因主要涉及能量代谢、氧化磷酸化等生物途径。STRING数据库分析结果显示,NDUFS3、ATP5D、ATP5I、NDUFB10等基因为相对核心的基因。结论 本研究通过生物信息学筛选出的巨噬细胞极化核心差异基因,为脓毒症的免疫学治疗提供新的方向。
Abstract:
Objective To find the differentially expressed genes between M1 and M2 subtypes of macrophage by bioinformatics methods and to provide a theoretical basis for screening the target of immunomodulatory therapy for sepsis.Methods Two microarray data were downloaded from gene expression omnibus(GEO) database.The samples were screened under the conditions of the macrophages polarized to M1 type under the action of interferon-gamma (IFN-γ) and lipopolysaccharide (LPS),and the macrophages polarized to M2 type under the action of interleukin-4(IL-4).The samples were submitted to the GEO2R platform for online analysis.The results of two gene expression profiles were cross-set to obtain the final differential expressed genes.The GO enrichment analysis and KEGG signaling pathway analysis of differentially expressed genes were performed by DAVID software and the protein-protein interaction was analysed by STRING database.Results A total of 1 241 differentially expressed genes were obtained.Among them,591 genes were up regulated in M1 macrophage while down regulated in M2 macrophage;and 650 were down regulated in M1 macrophage while up regulated in M2 macrophage.The function enrichment analysis suggested that the genes up regulated in M1 macrophage and down regulated in M2 macrophage were primarily involved in the inflammatory reaction and apoptosis,while the genes down regulated in M1 macrophage and up regulated in M2 macrophage were mainly involved in such biological processes as energy metabolism and oxidative phosphorylation.The STRING database analysis results showed that NDUFS3,CHCHD10,ATP5D,ATP5I,NDUFB10 and UQCR1 were relatively core genes.Conclusion The core differentially expressed genes of macrophage polarization which screened out by bioinformatics provide a new direction for the immunotherapy of sepsis.

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更新日期/Last Update: 2019-02-05