[1]段　峥,马东红,郭明好.全反式维甲酸对狼疮性肾炎小鼠的治疗作用[J].新乡医学院学报,2017,34(11):970-973.[doi:10.7683/xxyxyxb.2017.11.004]
　DUAN Zheng,MA Dong-hong,GUO Ming-hao.Therapeutic effect of all trans retinoic acid for lupus nephritis in mice[J].Journal of Xinxiang Medical University,2017,34(11):970-973.[doi:10.7683/xxyxyxb.2017.11.004]
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全反式维甲酸对狼疮性肾炎小鼠的治疗作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年11
页码:
970-973
栏目:
基础研究
出版日期:
2017-11-05

文章信息/Info

Title:
Therapeutic effect of all trans retinoic acid for lupus nephritis in mice
作者:
段　峥1; 马东红1; 郭明好2
（1.新乡医学院第一附属医院肾内科，河南　卫辉　453100；2.新乡医学院第三附属医院肾内科，河南　新乡　453003）
Author(s):
DUAN Zheng1; MA Dong-hong1; GUO Ming-hao2
（1.Department of Nephrology，the First Affiliated Hospital of Xinxiang Medical University，Weihui 453100，Henan Province，China；2.Department of Nephrology，the Third Affiliated Hospital of Xinxiang Medical University，Xinxiang 453003，Henan Province，China）
关键词:
全反式维甲酸; 环磷酰胺; 狼疮性肾炎; 单核细胞趋化蛋白-1
Keywords:
all-trans retinoic acid; cyclophosphamide; lupus nephritis; monocyte chemotactic protein-1
分类号:
R593.24+2
DOI:
10.7683/xxyxyxb.2017.11.004
文献标志码:
A
摘要:
目的　探讨全反式维甲酸（ATRA）对狼疮性肾炎（LN）小鼠的治疗作用。方法　将18只MRL/lpr小鼠随机分为对照组、环磷酰胺治疗组和ATRA治疗组，每组6只。对照组小鼠给予生理盐水灌胃（10 mg·kg-1·d-1），给药8周；ATRA治疗组小鼠给予ATRA灌胃（10 mg·kg-1·d-1），给药8周；环磷酰胺治疗组小鼠腹腔注射环磷酰胺（100 mg·kg-1·d-1），连续给药2 d。实验结束后采集小鼠血液及肾脏组织标本。使用全自动生物化学分析仪检测小鼠血尿素氮（BUN）、血肌酐（Scr）和尿肌酐（Ucr）水平，过碘酸雪夫染色观察肾组织结构变化，免疫组织化学法检测小鼠肾组织中转化生长因子-β1（TGF-β1）和单核细胞趋化蛋白-1（MCP-1）的表达。结果　ATRA治疗组和环磷酰胺治疗组小鼠Scr和BUN水平低于对照组，Ucr水平高于对照组（P<0.01）；ATRA治疗组小鼠BUN水平高于环磷酰胺治疗组，Ucr水平低于环磷酰胺治疗组（P<0.01）；ATRA治疗组和环磷酰胺治疗组小鼠Scr水平比较差异无统计学意义（P>0.05）。对照组小鼠可见肾小球基底膜增厚、系膜增生，肾小管基底膜增厚；环磷酰胺治疗组和ATRA治疗组小鼠也可见肾小球基底膜增厚、系膜增生，肾小管基底膜增厚，与对照组比较均有减轻，环磷酰胺治疗组减轻更明显。环磷酰胺治疗组和ATRA治疗组小鼠肾组织中MCP-1、TGF-β1表达均低于对照组（P<0.05）；ATRA治疗组小鼠肾组织中MCP-1、TGF-β1表达高于环磷酰胺治疗组（P<0.05）。结论　环磷酰胺和ATRA均可降低LN小鼠Scr、BUN水平，减轻肾脏病理改变，减少肾脏组织中TGF-β1和MCP-1的表达，但环磷酰胺效果优于ATRA。
Abstract:
Objective　To investigate the therapeutic effect of all-trans retinoic acid （ATRA） for lupus nephritis(LN) in mice.Methods　Eighteen MRL/lpr mice were randomly divided into control group，cyclophosphamide treatment group and ATRA treatment group，with 6 rats in each group.The mice in control group were administered saline （10 mg·kg-1·d-1） by intragastric administration for 8 weeks；the mice in ATRA treatment group were administered ATRA （10 mg·kg-1·d-1） by intragastric administration for 8 weeks；the mice in cyclophosphamide treatment group were given cyclophosphamide （100 mg·kg-1·d-1） by intraperitoneal injection for 2 days.Blood samples and kidney specimens were collected at the end of the experiment.The levels of blood urea nitrogen （BUN），serum creatinine （Scr） and urine creatinine （Ucr） were detected by full automatic biochemical analyzer；the changes of renal tissue structure was observed by periodic acid schiff；the expression of transforming growth factor-β1（TGF-β1） and monocyte chemotactic protein-1（MCP-1） was detected by immunohistochemistry.Results　The levels of Scr and BUN of mice in ATRA treatment group and cyclophosphamide treatment group were significantly lower than those in the control group，and the Ucr level was significantly higher than that in the control group（P<0.01）；the BUN level of mice in ATRA treatment group was significantly higher than that in the cyclophosphamide treatment group，but the Ucr level was significantly lower than that in the cyclophosphamide treatment group（P<0.01）；there was no statistic difference in the Scr level of mice between the ATRA treatment group and cyclophosphamide treatment group（P>0.05）.In control group，the glomerular basement membrane and renal tubular basement membrane of mice was thicken，the mesangial of mice was proliferated；the thicken glomerular basement membrane，renal tubular basement membrane and proliferated mesangial were also seen in ATRA treatment group and cyclophosphamide treatment group which was lighten than control group，and cyclophosphamide treatment group was lighten than ATRA treatment group.The expression of MCP-1，TGF-β1 of mice in cyclophosphamide treatment group and ATRA treatment group were significantly lower than those in the control group（P<0.05）；the expression of MCP-1，TGF-β1 of mice in ATRA treatment group were significantly higher than those in cyclophosphamide treatment group（P<0.05）.Conclusion　Both cyclophosphamide and ATRA can reduce the levels of Scr and BUN，lighten the pathological changes of renal and decrease the expression of TGF-β1 and MCP-1 in LN mice；but the effect of cyclophosphamide is better than ATRA.

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更新日期/Last Update: 2017-11-05