[1]吴向晖,黄鹏翀,秦海霞.miR-320靶向Rab11对宫颈癌细胞增殖、侵袭及迁移的影响[J].新乡医学院学报,2017,34(10):885-888.[doi:10.7683/xxyxyxb.2017.10.006]
　WU Xiang-hui,HUANG Peng-chong,QIN Hai-xia.miR-320 regulate proliferation,invasion and transfer of cervical cancer cells by targeting Rab11[J].Journal of Xinxiang Medical University,2017,34(10):885-888.[doi:10.7683/xxyxyxb.2017.10.006]
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miR-320靶向Rab11对宫颈癌细胞增殖、侵袭及迁移的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年10
页码:
885-888
栏目:
基础研究
出版日期:
2017-10-05

文章信息/Info

Title:
miR-320 regulate proliferation,invasion and transfer of cervical cancer cells by targeting Rab11
作者:
吴向晖1; 黄鹏翀1; 秦海霞2
（1.河南科技大学临床医学院　河南科技大学第一附属医院妇科,河南　洛阳　471003;2.新乡医学院第一附属医院妇科,河南　卫辉　453100）
Author(s):
WU Xiang-hui1; HUANG Peng-chong1; QIN Hai-xia2
（1.College of Clinical Medicine of Henan University of Science and Technology;Department of Gynecology,the First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003，Henan Province，China;2.Department of Gynecology,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100，Henan Province，China）
关键词:
miR-320; Rab11; 宫颈癌; 增殖; 侵袭
Keywords:
miR-320; Rab11; cervical cancer; proliferation; invasion
分类号:
R737.31
DOI:
10.7683/xxyxyxb.2017.10.006
文献标志码:
A
摘要:
目的　探讨miR-320对宫颈癌细胞SiHa增殖、侵袭及迁移的调控作用。方法　选取宫颈癌细胞系SiHa，将细胞分为miR-320模拟基因组、miR-320抑制剂组、对照模拟基因组、对照抑制剂组，各组分别转染miR-320 模拟基因、miR-320抑制剂、对照模拟基因、对照抑制剂。采用反转录聚合酶链式反应（RT-PCR）法检测各组SiHa细胞中miR-320表达;Western blot检测各组SiHa细胞中Rab11蛋白表达;噻唑蓝(MTT)法检测细胞增殖活性;Transwell小室实验检测细胞侵袭能力;划痕实验检测细胞迁移能力;双荧光素酶报告基因检测细胞荧光素酶活性。结果　miR-320 抑制剂组SiHa细胞中miR-320相对表达量低于对照抑制剂组（P<0.05）;miR-320模拟基因组SiHa细胞中miR-320相对表达量高于对照模拟基因组（P<0.05）。miR-320 抑制剂组SiHa细胞的细胞活性、侵袭能力、迁移能力高于对照抑制剂组（P<0.05）;miR-320模拟基因组SiHa细胞的细胞活性、侵袭能力、迁移能力低于对照模拟基因组（P<0.05）。miR-320抑制剂组SiHa细胞中Rab11蛋白表达高于对照抑制剂组（P<0.05）;miR-320模拟基因组SiHa细胞中Rab11蛋白表达低于对照模拟基因组（P<0.05）。miR-320抑制剂组SiHa细胞的细胞荧光活性显著低于对照抑制剂组（P<0.05）;miR-320模拟基因组SiHa细胞的细胞荧光活性与对照模拟基因组比较差异无统计学意义（P>0.05）。结论　miR-320可通过靶向Rab11调控SiHa细胞增殖、侵袭及迁移。
Abstract:
Objective　To evaluate the regulation role of miR-320 on proliferation,invasion and transfer of cervical cancer cells SiHa.Methods　The cervical cancer cells line SiHa were chosen and divided into miR-320 mimic group,miR-320 inhibitor group,control mimic group and control inhibitor;and the SiHa cells were transfected miR-320 mimic,miR-320 inhibitor,control mimic and control inhibitor respectively.The expression of miR-320 in SiHa cells in each group was detected by reverse transcription-polymerase chain reaction;the Rab11 protein expression was detected by Western blot;the proliferation activity of SiHa cells was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide method;the invasion ability of SiHa cells was detected by Transwell test;the transfer ability of SiHa cells was detected by scratch test;the cell luciferase activity was detected by dual-luciferase report gene experiment.Results　The expression of miR-320 in SiHa cells in miR-320 inhibitor group was significantly lower than that in control inhibitor group（P<0.05）;the expression of miR-320 in SiHa cells in miR-320 mimic group was significanlty higher than that in control mimic group（P<0.05）.The proliferation activity,invasion activity and transfer activity of SiHa cells in miR-320 inhibitor group were significantly higher than those in control inhibitor group（P<0.05）;the proliferation activity,invasion activity and transfer activity of SiHa cells in miR-320 mimic group were significanlty lower than those in control mimic group（P<0.05）.The Rab11 protein expression of SiHa cells in miR-320 inhibitor group was significantly higher than that in control inhibitor group（P<0.05）;the Rab11 protein expression of SiHa cells in miR-320 mimic group was significanlty lower than that in control mimic group（P<0.05）.The luciferase activity of SiHa cells in miR-320 inhibitor group was significantly lower than that in control inhibitor group（P<0.05）;there was no statistic difference in the luciferase activity of SiHa cells between miR-320 mimic group and control mimic group（P>0.05）.Conclusion　miR-320 can regulate the proliferation,invasion and transfer of SiHa cells by targeting Rab11.

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更新日期/Last Update: 2017-10-05