[1]温　辉.G蛋白信号调节因子5动态表达与大鼠胶质瘤生长的关系[J].新乡医学院学报,2017,34(7):560-564.[doi:10.7683/xxyxyxb.2017.07.002]
　WEN Hui.Relationship between the dynamic expression of regulator of G-protein signaling 5 and the growth of glioma in rats[J].Journal of Xinxiang Medical University,2017,34(7):560-564.[doi:10.7683/xxyxyxb.2017.07.002]
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G蛋白信号调节因子5动态表达与大鼠胶质瘤生长的关系
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年7
页码:
560-564
栏目:
基础研究
出版日期:
2017-07-05

文章信息/Info

Title:
Relationship between the dynamic expression of regulator of G-protein signaling 5 and the growth of glioma in rats
作者:
温　辉
（郑州大学附属洛阳市中心医院神经外科，河南　洛阳　471000）
Author(s):
WEN Hui
（Department of Neurosurgery，Luoyang Central Hospital Affiliated to Zhengzhou University，Luoyang 471000，Henan Province，China）
关键词:
大鼠; 胶质瘤; G蛋白信号调节因子5
Keywords:
rats; glioma; regulator of G-protein signaling 5
分类号:
R739.4
DOI:
10.7683/xxyxyxb.2017.07.002
文献标志码:
A
摘要:
目的　探讨G蛋白信号调节因子5（RGS5）动态表达与大鼠胶质瘤生长的关系。方法　Sprague-Dawley大鼠72只，随机分为正常对照组、生理盐水组、胶质瘤组和重组腺病毒组，每组18只。胶质瘤组和重组腺病毒组大鼠通过麻醉、钻孔开颅、注射C6胶质瘤细胞建立大鼠C6胶质瘤模型；生理盐水组大鼠麻醉、钻孔开颅方法同胶质瘤组及重组腺病毒组，仅注入生理盐水；正常对照组大鼠未给予任何干预措施。重组腺病毒组大鼠在接种C6胶质瘤细胞后第4、11天，于肿瘤细胞注射位置为肿瘤原位注射重组腺病毒Adv-rHSG-GFP。接种C6胶质瘤细胞后第9、15、21天，4组大鼠各取6只处死，取脑组织观察肿瘤的生长情况，免疫组织化学法检测RGS5蛋白的表达。结果　正常对照组、生理盐水组大鼠脑组织均未见肿瘤生长；胶质瘤组、重组腺病毒组大鼠在C6 胶质瘤细胞接种后的第9、15、21天，脑组织中均有胶质瘤生长。重组腺病毒组大鼠脑组织中肿瘤体积在第9、15、21天均小于相同时间点的胶质瘤组（P<0.05）；组内比较显示，胶质瘤组、重组腺病毒组大鼠在C6 胶质瘤细胞接种后第15、21天脑组织胶质瘤体积均大于第9天时（P<0.05），第21天的脑组织胶质瘤体积均大于第15天时（P<0.05）。正常对照组和生理盐水组大鼠脑组织中RGS5蛋白表达比较差异无统计学意义（P>0.05）。胶质瘤组、重组腺病毒组大鼠脑组织中RGS5蛋白表达在C6 胶质瘤细胞接种后的第9、15、21天均高于正常对照组和生理盐水组（P<0.05）；重组腺病毒组大鼠脑组织中的RGS5蛋白表达在第9、15、21天时均低于胶质瘤组相同时间点（P<0.05）；组内比较显示，胶质瘤组、重组腺病毒组大鼠脑组织中的RGS5蛋白表达在C6 胶质瘤细胞接种后的第15、21天时均高于第9天时（P<0.05），而第21天时均高于第15天时（P<0.05）。结论　RGS5表达与大鼠脑胶质瘤生长密切相关，Adv-rHSG-GFP对大鼠胶质瘤生长有重要的抑制作用。
Abstract:
Objective　To investigate the relationship between the dynamic expression of regulator of G-protein signaling 5 （RGS5) and the growth of glioma in rats.Methods　A total of 72 Sprague-Dawley rats were randomly divided into normal control group，saline group，glioma group and recombinant adenovirus group，with 18 rats in each group.The C6 glioma models of rats in glioma group and recombinant adenovirus group were established by injecting C6 glioma cells after anesthesia and drilling craniotomy.The rats in saline group were injected with physiological saline after anesthesia and drilling craniotomy.The rats in normal control group were not given any treatment measure.The recombinant adenovirus Adv-rHSG-GFP was injected into tumor cell site on the fourth and eleventh day after the inoculation of C6 glioma cells.Six rats in each group were sacrificed on the ninth，fifteenth and twenty-first day.The brain tissues were taken to observe the growth of the tumor，and the expression of RGS5 in the brain tissues was detected by immunohistochemical method.Results　There was no glioma in normal control group and saline group;the glioma was found in glioma group and recombinant adenovirus group on the ninth，fifteenth and twenty-first day.The volume of glioma in the recombinant adenovirus group was significantly less than that in glioma group on the ninth，fifteenth and twenty-first day （P<0.05).The volume of glioma on the fifteenth and twenty-first day after the inoculation of C6 glioma cells was significantly more than that on the ninth day in glioma group and recombinant adenovirus group（P<0.05).The volume of glioma on the twenty-first day after the inoculation of C6 glioma cells was significantly more than that on the fifteenth day in glioma group and recombinant adenovirus group（P<0.05).There was no significant difference in RGS5 protein expression in brain tissue of rats between the normal control group and the saline group （P>0.05).The RGS5 protein expression in brain tissue of rats in glioma group and recombinant adenovirus group was significantly higher than that in normal control group and the saline group on the ninth，fifteenth and twenty-first day after the inoculation of C6 glioma cells （P<0.05).The RGS5 protein expression in brain tissue of rats in recombinant adenovirus group was significantly lower than that in glioma group on the ninth，fifteenth and twenty-first day after the inoculation of C6 glioma cells （P<0.05).The RGS5 protein expression in brain tissue of rats on the fifteenth and twenty-first day after the inoculation of C6 glioma cells was significantly higher than that on the ninth day in glioma group and recombinant adenovirus group （P<0.05).The RGS5 protein expression in brain tissue of rats on the twenty-first day after the inoculation of C6 glioma cells was significantly higher than that on the fifteenth day in glioma group and recombinant adenovirus group （P<0.05).Conclusion　The expression of RGS5 is closely related to the growth of brain glioma，and Adv-rHSG-GFP plays an important role in inhibiting the growth of glioma in rats.

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更新日期/Last Update: 2017-07-05