[1]赵小云,黄　悦,赵少贞,等.先天性近视豚鼠眼球生物学参数及巩膜病理改变[J].新乡医学院学报,2017,34(6):465-468.[doi:10.7683/xxyxyxb.2017.06.003]
　ZHAO Xiao-yun,HUANG Yue,ZHAO Shao-zhen,et al.Biological parameters and the sclera pathological changes in congenital myopia guinea pigs[J].Journal of Xinxiang Medical University,2017,34(6):465-468.[doi:10.7683/xxyxyxb.2017.06.003]
点击复制

先天性近视豚鼠眼球生物学参数及巩膜病理改变
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年6
页码:
465-468
栏目:
基础研究
出版日期:
2017-06-05

文章信息/Info

Title:
Biological parameters and the sclera pathological changes in congenital myopia guinea pigs
作者:
赵小云1; 黄　悦2; 赵少贞2; 张未娟3; 张文静1
（1.武警后勤学院附属医院眼科，天津　300162；2.天津医科大学眼科医院眼科研究所，天津　300384；3.解放军第254医院眼科中心，天津　300000）
Author(s):
ZHAO Xiao-yun1; HUANG Yue2; ZHAO Shao-zhen2; ZHANG Wei-juan3; ZHANG Wen-jing1
（1.Department of Ophthalmology，the Affiliated Hospital of Logistics University of PAP，Tianjin 300162，China；2.Tianjin Medical University Eye Hospital，Eye Institute，Tianjin 300384，China；3.Eye Center of the No.254 Hospital of Chinese PLA，Tianjin 300000，China）
关键词:
先天性近视; 高度近视; 病理性近视; 豚鼠; 巩膜
Keywords:
congenital myopia; high myopia; pathological myopia; guinea pig; sclera
分类号:
R778.1+1
DOI:
10.7683/xxyxyxb.2017.06.003
文献标志码:
A
摘要:
目的　通过检测先天性近视豚鼠眼的屈光度、眼轴和巩膜病理改变，了解先天性近视豚鼠眼球的生物学特性。方法　于40只3周龄英国种短毛三色豚鼠中发现先天性近视豚鼠6只，验光示12眼的屈光度为-6.00～-12.00 D，以-10.00 D为界分为高度近视组（<-10.00 D，7眼）和超高度近视组（≥-10.00 D，5眼），同时选用6只同批正常视力豚鼠12眼作为正常对照组。各组均行视网膜检影测屈光度，A超测量眼球生物学参数，取眼球做病理切片并行光学显微镜检查。结果　正常对照组、高度近视组和超高度近视组豚鼠眼平均屈光度分别为（+2.38±1.54）、（-7.64±1.31）和（-10.80±0.76）D，3组豚鼠眼平均屈光度比较差异有统计学意义（F=216.305，P<0.05），且3组豚鼠眼平均屈光度两两比较差异均有统计学意义（P<0.05）。3组豚鼠眼球的前房深度和晶状体厚度比较差异均无统计学意义（F=0.112，P>0.05；F=0.100，P>0.05）；高度近视组、超高度近视组豚鼠眼球的玻璃体腔长度分别为（3.75±0.09）和（3.79±0.13）mm，显著长于正常对照组的（3.41±0.07）mm（F=50.306，P<0.05），但高度近视组与超高度近视组豚鼠眼球的玻璃体腔长度比较差异无统计学意义（P>0.05）；高度近视组、超高度近视组豚鼠的眼轴长度分别为（7.95±0.45）和（8.10±0.27）mm，显著长于正常对照组的（7.63±0.21）mm（F=4.924，P<0.05），但高度近视组与超高度近视组豚鼠的眼轴长度比较差异无统计学意义（P>0.05）。正常对照组豚鼠眼巩膜厚度正常，胶原纤维分布均匀，排列整齐，细胞外基质少；高度近视组豚鼠巩膜明显变薄，胶原纤维分布稀疏，排列紊乱，细胞外基质增多；超高度近视组较高度近视组以上改变更加显著。结论　先天性近视豚鼠眼的屈光状态、眼轴长度及巩膜病理学改变均符合病理性近视特点，可用于近视眼的相关研究。
Abstract:
Objective　To explore the biological characteristics of congenital myopia guinea pigs by detecting the diopter，axis oculi and the sclera pathology changes of guinea pigs.Methods　Six spontaneous congenital myopia guinea pigs were found in forty three-week-old pigmented guinea pigs（British shorthair stock）.The diopter of 12 eyes were -6.00--12.00 D.The 12 eyes were divided into high myopia group（diopter <-10.00 D，n=7） and super-high myopia group（diopter≥-10.00 D，n=5）.Another six normal pigmented guinea pigs （12 eyes） were selected as normal control group （n=12）.Diopter was detected by using retinoscopy；the biological parameters of eyes were measured by A-type sonography；the pathological section of eyeball was observed by light microscope.Results　The average diopter of pigmented guinea pigs in normal control group，high myopia group and super-high myopia group was （+2.38±1.54），（-7.64±1.31） and （-10.80±0.76）D respectively；there was statistic difference in the average diopter of pigmented guinea pigs among the three groups（F=216.305，P<0.05）.No significant differences were found in the anterior chamber depth and lens thickness of eyeball of pigmented guinea pigs among the three groups（F=0.112，P>0.05；F=0.100，P>0.05）.Vitreous cavity depth of eyeball of pigmented guinea pigs in high myopia group，super-high myopia group and normal control group was （3.75±0.09），（3.79±0.13） and （3.41±0.07）mm respectively；the vitreous cavity depth in high myopia group and super-high myopia group was significantly longer than that in the normal control group（F=50.306，P<0.05）；but there was no significant difference in the vitreous cavity depth between the high myopia group and super-high myopia group（P>0.05）.Axial oculi length of pigmented guinea pigs in high myopia group，super-high myopia group and normal control group was （7.95±0.45），（8.10±0.27） and （7.63±0.21）mm respectively；the axial oculi length in high myopia group and super-high myopia group was significantly longer than that in the normal control group（F=4.924，P<0.05）；but there was no significant difference in axis oculi length between the high myopia group and super-high myopia group（P>0.05）.In normal control group,the sclera thickness was normal，the distribution of collagen fiber was even,the collagen fiber arranged in order,and the extracellular matrix was less.In the high myopia group，the sclera was thinner；the collagen fiber distribution and arrangement was sparse，slender and disordered，and there was more extracellular matrix.The above changes in super-high myopia group was more significant.Conclusion　The refractive status，axis oculi length and the morphological changes of sclera in guinea pigs with spontaneous congenital myopia are in consistence with the changes of pathological myopia.So guinea pigs with spontaneous congenital myopia can be used for researching myopia.

参考文献/References:

［1］　PAN C W，RAMAMURTHY D，SAW S M.Worldwide prevalence and risk factors for myopia［J］.Ophthalmic Physiological Optics，2012，32（1）：3-16.
［2］　HOLDEN B，SANKARIDURG P，SMITH E，et al.Myopia，an underrated global challenge to vision：where the current data takes US on myopia control［J］.Eye，2014，28（2）：142-146.
［3］　MITRY D，ZAMBARAKJI H.Recent trends in the management of maculopathy secondary to pathological myopia［J］.Graefes Arch Clin Exp Ophthalmol，2012，250（1）：3-13.
［4］　龙克利，蒋丽琴，李翌，等.豚鼠形觉剥夺性近视发生及恢复期眼球生物参数变化的研究［J］.中华眼科杂志，2010，46（6）：550-555.
［5］　汪芳润，尹忠贵，杨晨皓.论近视眼的分类［J］.眼科，2007，16（5）：294-297.
［6］　庄文娟.中国人群IGF-1基因多态性与高度近视的相关性研究［D］.重庆：重庆医科大学，2012.
［7］　袁南菱，王超英，贺江美，等.高度近视眼轴的初步研究［J］.中国实用眼科杂志，1998，16（5）：290.
［8］　FROST M R，NORTON T T.Alterations in protein expression in tree shrew sclera during development of lens-induced myopia and recovery［J］.Invest Ophthalmol Vis Sci，2012，53（1）：322-336.
［9］　李勇子，刘桂香，王玲，等.TIMP-2基因转染FDM豚鼠模型早期后极部巩膜中MMP-2的动态表达［J］.眼科新进展，2012，32（12）：1104-1109.
［10］　李国平，吴建峰，叶翔，等.负透镜诱导型近视豚鼠视网膜中谷氨酸及其受体的表达［J］.眼科新进展，2016，36（10）：910-914.
［11］　曾官鹏，严丽英，徐双，等.MFN1在透镜诱导豚鼠近视模型视网膜上的表达及其意义［J］.眼科新进展，2016，36（1）：1-3，11.
［12］　李传旭，崔冬梅，霍丽君，等.外源性视黄酸诱导的豚鼠近视模型的建立［J］.眼科新进展，2012，32（8）：705-707.
［13］　史剑波，徐锦堂，夏潮涌，等.豚鼠视网膜正常结构的定量研究［J］.眼科研究，1999，17（2）：98-100.
［14］　余继锋，马飞，龙潭，等.正常豚鼠视网膜电图的特点及记录方法［J］.眼科新进展，2005，25（6）：535-537.
［15］　田小芸，吴波，章如松，等.先天性白内障大鼠晶状体的病理学研究［J］.医学研究生学报，2015，28（8）：820-823.
［16］　田小芸，董敏，尤金炜，等.先天性白内障大鼠培育初报［J］.实验动物与比较医学，2013，33（5）：358-360.
［17］　田小芸，尤金炜，董敏，等.先天性白内障大鼠的繁殖性能及其生长发育［J］.中国比较医学杂志，2013，23（12）：10-12.
［18］　田小芸，赵志刚，许龙祥，等.先天性白内障大鼠主要脏器重量与脏器系数［J］.实验动物与比较医学，2014，34（5）：1-3.
［19］　田小芸，尤金炜，董敏，等.先天性白内障大鼠血液生化及血液流变学分析［J］.中国比较医学杂志，2014，24（3）：20-24.

相似文献/References:

[1]袁小艾,王保君,周予兰,等.调整切削屈光度对飞秒激光辅助准分子激光原位角膜磨镶术治疗高度近视疗效的影响[J].新乡医学院学报,2019,36(10):975.[doi:10.7683/xxyxyxb.2019.10.017]
　YUAN Xiao-ai,WANG Bao-jun,ZHOU Yu-lan,et al.Effects of femtosecond laser-assisted excimer laser in situ keratomileusis in the treatment of high myopia adjusted cutting diopter during operation[J].Journal of Xinxiang Medical University,2019,36(6):975.[doi:10.7683/xxyxyxb.2019.10.017]

更新日期/Last Update: 2017-06-05