[1]郝志勇,千智斌.5-羟色胺2C受体对新生大鼠离体延髓脑片基本节律性呼吸放电的调节作用[J].新乡医学院学报,2017,34(4):261-264.[doi:10.7683/xxyxyxb.2017.04.004]
　HAO Zhi-yong,QIAN Zhi-bin.Regulatory effect of 5-hydroxytryptamine 2C receptor on the rhythmic respiratory discharge activity of medulla slice of neonatal rat in vitro[J].Journal of Xinxiang Medical University,2017,34(4):261-264.[doi:10.7683/xxyxyxb.2017.04.004]
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5-羟色胺2C受体对新生大鼠离体延髓脑片基本节律性呼吸放电的调节作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年4
页码:
261-264
栏目:
基础研究
出版日期:
2017-04-05

文章信息/Info

Title:
Regulatory effect of 5-hydroxytryptamine 2C receptor on the rhythmic respiratory discharge activity of medulla slice of neonatal rat in vitro
作者:
郝志勇1; 千智斌2
（1.濮阳市人民医院神经外科，河南　濮阳　457000；2.新乡医学院机能学实验室，河南　新乡　453003）
Author(s):
HAO Zhi-yong1; QIAN Zhi-bin2
（1.Department of Neurosurgery，the People′s Hospital of Puyang，Puyang 457000，Henan Province，China；2.Functional Lab of Xinxiang Medical University，Xinxiang 453003，Henan Province，China）
关键词:
5-羟色胺2C受体; 新生大鼠; 延髓脑片; 基本节律性呼吸放电
Keywords:
5-hydroxytryptamine 2C receptor; neonatal rat; medullary slice; rhythmic respiratory discharge activity
分类号:
R722.1
DOI:
10.7683/xxyxyxb.2017.04.004
文献标志码:
A
摘要:
目的　探讨5-羟色胺2C（5-HT2C）受体对新生大鼠离体延髓脑片基本节律性呼吸放电（RRDA）的调节作用。方法　取2日龄Sprague-Dawley大鼠18只制备离体延髓脑片标本，使用人工脑脊液（ACSF）灌流脑片，吸附电极记录脑片舌下神经根RRDA。观察使用空白ACSF灌流脑片15、30、45、60 min时RRDA的吸气时程（TI）、呼吸周期（RC）和放电积分幅度（IA）变化情况；使用含1、5、10、20 μmol·L-1 5-HT2C受体激动剂MK212的ACSF灌流脑片后 RRDA的TI、RC和IA变化情况；使用含1、5、10、20 μmol·L-1 5-HT2C受体阻断剂RS102221的ACSF灌流脑片后RRDA的TI、RC和IA变化情况。结果　空白ACSF灌流脑片15、30、45、60 min时RRDA的TI、RC和IA比较差异均无统计学意义（P>0.05）。1 μmol·L-1 MK212的ACSF灌流脑片后RRDA的TI、IA和RC与给药前比较差异均无统计学意义（P>0.05）；5 μmol·L-1 MK212的ACSF灌流脑片后RRDA表现为TI延长、IA增加、RC缩短（P<0.05）；10 μmol·L-1 MK212的ACSF灌流脑片后RRDA表现较5 μmol·L-1 MK212的ACSF灌流脑片后TI进一步延长、IA进一步增加、RC进一步缩短（P<0.05）；但20 μmol·L-1 MK212的ACSF灌流脑片后与10 μmol·L-1 MK212的ACSF灌流脑片后比较，RRDA的TI、IA、RC差异均无统计学意义（P>0.05）。1 μmol·L-1 RS102221的ACSF灌流脑片后RRDA的TI、IA和RC与给药前比较差异均无统计学意义（P>0.05）；5 μmol·L-1 RS102221的ACSF灌流脑片后RRDA表现为TI缩短、RC延长（P<0.05），IA差异无统计学意义（P>0.05）；10 μmol·L-1 RS102221的ACSF灌流脑片后RRDA表现较5 μmol·L-1 RS102221的ACSF灌流脑片后TI缩短、IA减少、RC延长（P<0.05）；但20 μmol·L-1 RS102221的ACSF灌流脑片后与10 μmol·L-1 RS102221的ACSF灌流脑片后比较，RRDA的TI、IA、RC差异均无统计学意义（P>0.05）。结论　5-HT2C受体对延髓呼吸中枢起着正性调节作用。
Abstract:
Objective　To explore the regulatory effect of 5-hydroxytryptamine 2C（5-HT2C）receptor on the rhythmic respiratory discharge activity（RRDA） of medulla slice of neonatal rat in vitro.Methods　Eighteen Sprague-Dawley rats（2 days old） were taken to prepare the medullary slices specimen.The medullary slices were perfused with artificial cerebrospinal fluid（ACSF）,and the RRDA of sublingual nerve root was recorded by suction electrode.The inspiratory time（TI），respiratory cycle（RC） and integral amplitude（IA） of RRDA of medulla slices at the time points of 15，30，45，60 min after being perfused blank ACSF were observed.The TI，RC and IA of RRDA of medulla slices after being perfused ACSF which contained 1，5，10，20 μmol·L-1 5-HT2C receptor agonist MK212 respectively were observed.The TI，RC and IA of RRDA of medulla slices after being perfused ACSF which contained 1，5，10，20 μmol·L-1 5-HT2C receptor agonist RS102221 respectively was observed.Results　There was no statistic difference in the TI，RC and IA of RRDA of medulla slices among the time points of 15，30，45，60 min after being perfused blank ACSF（P>0.05）.There was no statistic difference in the TI，RC and IA of RRDA of medulla slices before and after being perfused 1 μmol·L-1 MK212 ACSF（P>0.05）；the TI，RC and IA of RRDA of medulla slices after being perfused 5，10，20 μmol·L-1 MK212 ACSF were significantly bigger than before and after being perfused 1 μmol·L-1 MK212 ACSF（P<0.05）；the TI，RC and IA of RRDA of medulla slices after being perfused 10，20 μmol·L-1 MK212 ACSF were significantly bigger than after being perfused 5 μmol·L-1 MK212 ACSF（P<0.05）；there were no statistic difference in the TI，RC and IA of RRDA of medulla slices after being perfused 10 μmol·L-1 MK212 ACSF and 20 μmol·L-1 MK212 ACSF（P>0.05）.There was no statistic difference in the TI，RC and IA of RRDA of medulla slices before and after being perfused 1 μmol·L-1 RS102221 ACSF（P>0.05）；the TI，RC of RRDA of medulla slices after being perfused 5 μmol·L-1 RS102221 ACSF was significantly bigger than before and after being perfused 1 μmol·L-1 RS102221 ACSF（P<0.05），there was no statistic difference in IA of RRDA of medulla slices before and after being perfused 1 μmol·L-1 RS102221 ACSF（P>0.05）；the TI，RC and IA of RRDA of medulla slices after being perfused 10，20 μmol·L-1 RS102221 ACSF were significantly shorter than after being perfused 1，5 μmol·L-1 RS102221ACSF（P<0.05）；there was no statistic difference in the TI，RC and IA of RRDA of medulla slices after being perfused 10 μmol·L-1 MK212 ACSF and 20 μmol·L-1 MK212 ACSF（P>0.05）.Conclusion　5HT2C receptor has positive regulation role on RRDA of medulla slice of neonatal rat.

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更新日期/Last Update: 2017-04-05