[1]徐海环,董化江,赵明亮.脐带间充质干细胞上清提取物对糖尿病皮肤溃疡大鼠肿瘤坏死因子-α及血管内皮生长因子的影响[J].新乡医学院学报,2017,34(2):090-93.[doi:10.7683/xxyxyxb.2017.02.003]
　XU Hai-huan,DONG Hua-jiang,ZHAO Ming-liang.Effect of supernatant of umbilical cord mesenchymal stem cells on tumor necrosis factor-α and vascular endothelial growth factor in diabetic skin ulcer rats[J].Journal of Xinxiang Medical University,2017,34(2):090-93.[doi:10.7683/xxyxyxb.2017.02.003]
点击复制

脐带间充质干细胞上清提取物对糖尿病皮肤溃疡大鼠肿瘤坏死因子-α及血管内皮生长因子的影响
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年2
页码:
090-93
栏目:
基础研究
出版日期:
2017-02-05

文章信息/Info

Title:
Effect of supernatant of umbilical cord mesenchymal stem cells on tumor necrosis factor-α and vascular endothelial growth factor in diabetic skin ulcer rats
作者:
徐海环1; 2; 董化江1; 赵明亮3
(1.天津大学精密测试技术及仪器国家重点实验室，天津　300072；2.武警后勤学院附属医院皮肤科，天津　300162；3.天津市神经修复重点实验室，天津　300162)
Author(s):
XU Hai-huan1; 2; DONG Hua-jiang1; ZHAO Ming-liang3
(1.State Key Laboratory of Precision Measurement Technology and Instruments，Tianjin University，Tianjin 300072，China；2.Department of Dermatology，the Affiliated Hospital of Logistics University of Chinese People′s Armed Police Forces，Tianjin 300162，China；3.Neural Restoration Key Laboratory of Tianjin，Tianjin 300162，China)
关键词:
脐带; 间充质干细胞; 糖尿病皮肤溃疡; 肿瘤坏死因子-α; 血管内皮生长因子; 大鼠
Keywords:
umbilical cord; mesenchymal stem cells; diabetic skin ulcer; tumor necrosis factor-α; vascular endothelial growth factor; rats
分类号:
R587.1R753
DOI:
10.7683/xxyxyxb.2017.02.003
文献标志码:
A
摘要:
目的　探讨脐带间充质干细胞上清提取物对糖尿病皮肤溃疡大鼠肿瘤坏死因子-α（TNF-α）及血管内皮生长因子（VEGF）的影响。方法　将30只Sprague-Dawley大鼠随机分为模型组、单纯培养基冻干粉治疗组和间充质干细胞上清冻干粉治疗组，每组10只。大鼠经尾静脉注射四氧嘧啶并给予高脂饲料喂养制作糖尿病模型。糖尿病大鼠采用戊巴比妥钠麻醉后，用刀片在小腿处划一切口，深达皮下，注入金黄色葡萄球菌悬液，造成皮肤溃疡。皮肤溃疡模型制作成功后模型组大鼠尾静脉注射100 μL生理盐水，溃疡处滴加100 μL生理盐水；单纯培养基冻干粉治疗组大鼠将培养基冻干粉溶解至200 μL生理盐水，尾静脉注射100 μL，溃疡处滴加100 μL；间充质干细胞上清冻干粉治疗组大鼠将间充质干细胞上清冻干粉溶解至200 μL生理盐水，尾静脉注射100 μL，溃疡处滴加100 μL。5 d后采用放射免疫法检测大鼠血清TNF-α水平，采用反转录-聚合酶链式反应和Western blot检测大鼠溃疡处组织中TNF-α和VEGF的表达。结果　间充质干细胞上清冻干粉治疗组大鼠血清TNF-α水平显著低于模型组和单纯培养基冻干粉治疗组（P<0.05）；模型组与单纯培养基冻干粉治疗组大鼠血清TNF-α水平比较差异无统计学意义（P>0.05）。间充质干细胞上清冻干粉治疗组大鼠溃疡组织中TNF-α mRNA及蛋白表达低于模型组和单纯培养基冻干粉治疗组（P<0.05）；间充质干细胞上清冻干粉治疗组大鼠溃疡组织中VEGF mRNA及蛋白的表达高于模型组和单纯培养基冻干粉治疗组（P<0.05）；模型组大鼠溃疡组织中TNF-α mRNA、VEGF mRNA及相应蛋白表达与单纯培养基冻干粉治疗组比较差异无统计学意义（P>0.05）。结论　间充质干细胞上清冻干粉治疗可有效降低溃疡组织中TNF-α的表达，且可有效提高溃疡组织中VEGF的表达水平。
Abstract:
Objective　To study the effect of supernatant of umbilical cord mesenchymal stem cells(MSCs) on tumor necrosis factor-α(TNF-α) and vascular endothelial growth factor(VEGF) in diabetic skin ulcer（DSU） rats.Methods　Thirty Sprague-Dawley rats were randomly divided into model group，culture medium treatment group and MSCs supernatant treatment group，with 10 rats in each group.The diabetic rat models were made by injecting alloxan through caudal vein and giving high-fat diet.The diabetic rats were anesthetized with pentobarbital sodium；and then were cut a wound by the blade in the calf，the depth to the subcutaneous tissue.The wounds were infused the suspension of Staphylococcus aureus to form the skin ulcer.After eatblishing the skin ulcer models，the rats in model group were injected physiological saline 100 μL through caudal vein；at the same time，the physiological saline 100 μL was droped to the ulcer tissure.In the culture medium treatment group，the freeze-dried powder of culture medium was dissolved in 200 μL physiological saline，an then 100 μL was infused to rats through caudal vein，100 μL was droped to the ulcer tissure.In MSCs supernatant treatment group，the freeze-dried powder of supernatant was dissolved in 200 μL physiological saline，and then 100 μL was infused to rats through caudal vein，100 μL was droped to the ulcer tissure.After five days，the serum levels of TNF-α was detected by radioimmunoassay；the expression of VEGF and TNF-α in ulcer tissure of rats was measured by reverse transcription polymerase chain reaction and Western blotting.Results　The serum level of TNF-α of rats in MSCs supernatant treatment group was significanlty lower than that in model group and culture medium treatment group（P<0.05）；there was no statistic difference in serum level of TNF-α of rats between the model group and culture medium treatment group（P>0.05）.The expressions of TNF-α mRNA and protein in ulcer tissure of rats in MSCs supernatant treatment group were significanlty lower than those in model group and culture medium treatment group（P<0.05）；the expressions of VEGF mRNA and protein in ulcer tissure of rats in MSCs supernatant treatment group were significanlty higher than those in model group and culture medium treatment group（P<0.05）；there was no statistic difference in the expressions of VEGF and TNF-α mRNA and protein in ulcer tissure of rats between the model group and culture medium treatment group（P>0.05）.Conclusion　Umbilical cord MSCs can effectively reduce the expression of TNF-α and increase the expression of VEGF in ulcer tissure.

参考文献/References:

［1］　李晶艳，周琦，吕红彬.糖尿病早期视网膜神经元退行性病变最新研究进展［J］.眼科新进展，2015，35(5)：493-496.
［2］　沈盛县，严宏.糖尿病性白内障与视网膜病变联合治疗的时机和策略［J］.眼科新进展，2015，35(8)：791-794.
［3］　张云华，陆汉军.2型糖尿病及其并发症中西医结合诊疗与预防［M］.武汉：华中科技大学出版社，2009：1-2.
［4］　蔡丽萍，孟然，张宏.眼局部应用骨髓间充质干细胞(BMSC)治疗小鼠干眼的实验研究［J］.眼科新进展，2016，36(11)：1024-1028.
［5］　SCHOUTEN J W，FULP C T，ROYO N C，et al.A review and rationale for the use of cellular transplantation as a therapeutic strategy for traumatic brain injury［J］.J Neurotrauma，2004，21(11)：1501-1538.
［6］　PITTENGER M F，MACKAY A M，BECK S C，et al.Multilineage potentialo of adult human mesenchymal stem cells［J］.Science，1999，284（5411）：143-147.
［7］　MARESCHI K，BIASIN E，PIACIBELLO W，et al.Isolation of human mesenchymal stem cells：bone marrow versu umbilical cord blood［J］.Hematological，2001，86(10)：1099-1100.
［8］　LI J P，WAN D W，SONG Q H.Transplantation of erythropoietin gene-transfected umbilical cord mesenchymal stem cells as a treatment for limb ischemia in rats［J］.Genet Mol Res，2015，14(4)：19005-19015.
［9］　张永文，张洁，吴秉司，等.丹参酮ⅡA磺酸钠注射液治疗糖尿病足疗效观察［J］.新乡医学院学报，2016，33(2)：142-144，147.
［10］　于鑫，董化江，单娜娜，等.三七总甙对糖尿病皮肤溃疡大鼠肿瘤坏死因子-α水平的影响［J］.新乡医学院学报，2012，29(12)：893-895.
［11］　杨汉华，陈元国，赖秀蓝，等.六个转录因子将人脐带间充质干细胞高效重编程为诱导性多能干细胞的实验研究［J］.中华实用儿科临床杂志，2014，29（17）：1331-1336.
［12］　CAPLAN A L.Mesenchymal stem cells［J］.Orthop Res，1991，9(5)：641-650.
［13］　HE H，ZHAO Z H，HAN F S，et al.Overexpression of protein kinase C ε improves retention and survival of transplanted mesenchymal stem cells in rat acute myocardial infarction［J］.Cell Death Disease，2016，7(1)：e2056.
［14］　LEE R H，PULIN A A，SEO M J，et al.Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6［J］.Cell Stem Cell，2009，5(1)：54-63.
［15］　常丽，刘卓，王玉平，等.闭塞性细支气管炎患儿血清血管内皮生长因子的表达及其与病程的相关性［J］.中华实用儿科临床杂志，2016，31（12）：947-948.

相似文献/References:

[1]郭瑞敏,曹毓琳,程世翔,等.间充质干细胞源性外泌体在呼吸系统疾病中的应用与机制研究进展[J].新乡医学院学报,2021,38(11):1084.[doi:10.7683/xxyxyxb.2021.11.017]
[2]孙钰椋,刘彦礼,林俊堂.经血源子宫内膜干细胞在新型冠状病毒肺炎治疗中的应用前景[J].新乡医学院学报,2020,37(3):201.[doi:10.7683/xxyxyxb.2020.03.001]
[3]河南省细胞生物学会干细胞专业委员会,中国研究型医院学会临床数据与样本资源库专业委员会.经血源子宫内膜干细胞资源库建立标准专家共识[J].新乡医学院学报,2020,37(9):801.[doi:10.7683/xxyxyxb.2020.09.001]
[4]刘彦礼,曹毓琳,林俊堂.经血源子宫内膜干细胞生物学特性及临床应用研究进展[J].新乡医学院学报,2020,37(9):885.[doi:10.7683/xxyxyxb.2020.09.019]
[5]赵　欣,谭　军.间充质干细胞治疗缺血性脑卒中临床研究现状[J].新乡医学院学报,2017,34(6):545.[doi:10.7683/xxyxyxb.2017.06.026]
[6]刘　阳,傅　强.骨髓间充质干细胞成骨分化调控机制研究进展[J].新乡医学院学报,2016,33(5):443.[doi:10.7683/xxyxyxb.2016.05.027]
　LIU Yang,FU Qiang.Research progress on the regulation mechanism of osteogenic differentiation of bone marrow mesenchymal stem cells[J].Journal of Xinxiang Medical University,2016,33(2):443.[doi:10.7683/xxyxyxb.2016.05.027]
[7]班跃耀,孙蕾,马保东,等.促红细胞生成素基因修饰的人脐带间充质干细胞对神经元周期和凋亡的调控作用及机制[J].新乡医学院学报,2023,40(10):909.[doi:10.7683/xxyxyxb.2023.10.002]
　BAN Yueyao,SUN Lei,MA Baodong,et al.Regulatory effect and mechanism of human umbilical cord mesenchymal stem cells modified with erythropoietin gene on cell cycle and apoptosis of neuron[J].Journal of Xinxiang Medical University,2023,40(2):909.[doi:10.7683/xxyxyxb.2023.10.002]
[8]徐海环,董化江,赵明亮.脐带间充质干细胞上清冻干粉对大耳兔皮肤缺损的治疗作用[J].新乡医学院学报,2016,33(12):1041.[doi:10.7683/xxyxyxb.2016.12.006]
　XU Hai-huan,DONG Hua-jiang,ZHAO Ming-liang.Effect of supernatant of umbilical cord mesenchymal stem cells on skin defect in flapeared rabbits[J].Journal of Xinxiang Medical University,2016,33(2):1041.[doi:10.7683/xxyxyxb.2016.12.006]

更新日期/Last Update: 2017-02-05