[1]张刘源,陈贵梅,魏向娟,等.D-核糖交联胶原/纤维蛋白胶复合支架的制备及体外性能研究[J].新乡医学院学报,2016,33(12):1031-1035.[doi:10.7683/xxyxyxb.2016.12.004]
　ZHANG Liu-yuan,CHEN Gui-mei,WEI Xiang-juan,et al.Preparation and characteration in vitro of D-ribose crosslinked collagen /fibrin composite scaffolds[J].Journal of Xinxiang Medical University,2016,33(12):1031-1035.[doi:10.7683/xxyxyxb.2016.12.004]
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D-核糖交联胶原/纤维蛋白胶复合支架的制备及体外性能研究
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年12
页码:
1031-1035
栏目:
基础研究
出版日期:
2016-12-05

文章信息/Info

Title:
Preparation and characteration in vitro of D-ribose crosslinked collagen /fibrin composite scaffolds
作者:
张刘源1; 陈贵梅1; 魏向娟1; 2; 张丹丹1; 刘会芳1; 刘宇倩1; 南文滨1; 2; 陈红丽1; 2
（1.新乡医学院生命科学技术学院，河南　新乡　453003；2.河南省干细胞与生物治疗工程技术研究中心，河南　新乡　453003）
Author(s):
ZHANG Liu-yuan1; CHEN Gui-mei1; WEI Xiang-juan1; 2; ZHANG Dan-dan1; LIU Hui-fang1; LIU Yu-qian1; NAN Wen-bin1; 2; CHEN Hong-li1; 2
（1.School of Life Science and Technology,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;2.Stem Cell and Biotherapy Technology Research Center of Henan Province,Xinxiang 453003,Henan Province,China）
关键词:
D-核糖; 交联; 胶原; 纤维蛋白; 脐带间充质干细胞
Keywords:
D-ribose; crosslinked; collagen; fibrin; mesenchymal stem cells
分类号:
R318
DOI:
10.7683/xxyxyxb.2016.12.004
文献标志码:
A
摘要:
目的　制备与D-核糖交联的胶原/纤维蛋白胶复合支架，并探讨含有不同浓度D-核糖的交联液对复合支架体外性能的影响。方法　根据交联液中D-核糖浓度将制备的复合支架分为对照组和A、B、C、D、E组。对照组为未交联的复合支架；A、B、C、D组胶原分别与含不同浓度(0.1、0.2、0.4、0.8 mol·L-1)D-核糖的交联液在室温条件下交联24 h；E组胶原与戊二醛在室温条件下交联24 h。观察交联剂浓度对复合支架的机械性能、降解特性等的影响。采用细胞活力试剂盒（CCK-8）检测复合支架的浸提液对脐带间充质干细胞活性的影响。结果　C、D、E组孔隙率与对照组及A、B组比较差异均有统计学意义（P<0.05）；C、D、E组孔隙率两两比较差异均无统计学意义（P>0.05）;对照组及A、B组孔隙率两两比较差异亦均无统计学意义（P>0.05）。A、B、C、D、E组复合支架的拉伸强度均高于对照组，断裂伸长率均低于对照组（P<0.05）；B、C、D、E组复合支架的拉伸强度均高于A组，断裂伸长率均低于A组（P<0.05）；C、D、E组复合支架的拉伸强度均高于B组，断裂伸长率均低于B组（P<0.05）;C、D、E 3组复合支架的拉伸强度和断裂伸长率两两比较差异均无统计学意义（P>0.05）。对照组及A、B、C、D、E 组在各时间点的降解质量百分率均呈现降低的趋势。C、D、E组复合支架在4、8、16、24、32、40和 48 h 时降解质量百分率均低于对照组及A、B组（P<0.05）；C、D、E组复合支架在各时间点的降解质量百分率两两比较差异均无统计学意义（P>0.05）。各组24、48 h时细胞活力比较差异均无统计学意义（P>0.05）。对照组及A、B、C、D组在24、48 h细胞活力均高于E组（P<0.05）；对照组及A、B、C、D组在24、48 h细胞活力两两比较差异均无统计学意义（P>0.05）。结论　D-核糖作为交联剂，浓度为0.4 mol·L-1即能较好改善胶原/纤维蛋白胶复合支架的体外机械性能和降解速率，且细胞毒性小。
Abstract:
Objective　To prepare D-ribose crosslinked collagen /fibrin composite scaffolds and explore the effect of concentration of D-ribose on the properties of the composite scaffolds in vitro.Methods　The composite scaffolds were divided into control group(non crosslinked composite scaffolds)，group A(collagen crosslinked with 0.1 mol·L-1 D-ribose cross-linking liquid under room temperature for 24 h)，group B(collagen crosslinked with 0.2 mol·L-1 D-ribose cross-linking liquid under room temperature for 24 h)，group C(collagen crosslinked with 0.4 mol·L-1 D-ribose cross-linking liquid under room temperature for 24 h)，group D(collagen crosslinked with 0.8 mol·L-1 D-ribose cross-linking liquid under room temperature for 24 h) and group E(collagen crosslinked with glutaral under room temperature for 24 h).The effect of the concentration of D-ribose on the properties(such as mechanical function,degradation degradation) of the composite scaffolds was observed.The effect of the leach liquor of composite scaffolds on the activity of human umbilical cord mesenchymal stem cell in vitro was evaluated by using cell counting kit-8(CCK-8) assay.Results　There was statistic difference of the poriness of composite scaffolds between the group C,D,E and control group,group A,B(P<0.05);there was no statistic difference in the poriness of composite scaffolds among the group C,D,E（P>0.05）;there was no statistic difference in the poriness of composite scaffolds among the control group,group A,B（P>0.05）.The tensile strength of composite scaffolds in group A,B,C,D,E was significanlty higher than that in the control group;but the breaking elongating rate was significantly lower than that in control group（P<0.05）.The tensile strength of the composite scaffolds in group B,C,D,E was significanlty higher than that in group A;but the breaking elongating rate was significantly lower than that in group A（P<0.05）.The tensile strength of the composite scaffolds in group C,D,E was significanlty higher than that in group B;but the breaking elongating rate was significantly lower than that in group B（P<0.05）.There were no statistic difference in the tensile strength and breaking elongating rate of the composite scaffolds among the the group C,D,E（P>0.05）.The degraded mass percentage of composite scaffolds in control group,group A,B,C,D,E showed a decreasing trend at 4,8,16,24,32,40 and 48 h.The degraded mass percentage of composite scaffolds in group C,D,E was significanlty lower than that in control group and group A,B（P<0.05）;there was no statistic difference in the degraded mass percentage of composite scaffolds among the group C,D,E（P>0.05）.There was no statistic difference in the cell viability between the 24 h and 48 h in each group（P>0.05）.The cell viability in control group,group A,B,C,D was significanlty higher than that in group E（P<0.05）;there was no statistic difference in the cell viability among control group,group A,B,C,D（P>0.05）.Conclusion　D-ribose as a crosslinking agent，it can better improve the mechanical properties and degradation rate of collagen/fibrin composite scaffolds and it′s cytotoxicity is small.

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更新日期/Last Update: 2016-12-05