[1]杨伟锋,金保哲,张新中,等.丙戊酸对颅脑损伤大鼠海马成体神经干细胞原位激活的作用[J].新乡医学院学报,2016,33(6):469-472.[doi:10.7683/xxyxyxb.2016.06.006]
 YANG Wei-feng,JIN Bao-zhe,ZHANG Xin-zhong,et al.Effect of valproic acid on the situ activation of hippocampal adult neural stem cells in brain tissue of rats with experimental traumatic brain injury[J].Journal of Xinxiang Medical University,2016,33(6):469-472.[doi:10.7683/xxyxyxb.2016.06.006]
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丙戊酸对颅脑损伤大鼠海马成体神经干细胞原位激活的作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年6
页码:
469-472
栏目:
基础研究
出版日期:
2016-06-05

文章信息/Info

Title:
Effect of valproic acid on the situ activation of hippocampal adult neural stem cells in brain tissue of rats with experimental traumatic brain injury
作者:
杨伟锋1金保哲2张新中2王 磊1周文科2王仲伟2
(1.新乡医学院2013级硕士研究生,河南 新乡 453003;2.新乡医学院第一附属医院神经外科,河南 卫辉 453100)
Author(s):
YANG Wei-feng1JIN Bao-zhe2ZHANG Xin-zhong2WANG Lei1ZHOU Wen-ke2WANG Zhong-wei2
(1.Grade 2013,Graduate Student of Xinxiang Medical University,Xinxiang 453003,Henan Province,China;2.Department of Neurosurgery,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China)
关键词:
创伤性颅脑损伤丙戊酸大鼠神经干细胞
Keywords:
traumatic brain injuryvalproic acidratneural stem cells
分类号:
R394.2
DOI:
10.7683/xxyxyxb.2016.06.006
文献标志码:
A
摘要:
目的 观察丙戊酸(VPA)对颅脑损伤后大鼠海马成体神经干细胞(NSC)原位激活的作用。方法 将成年健康Sprague-Dawley雄性大鼠66只随机分为正常对照组(n=6)、单纯损伤组(n=30)和VPA治疗组(n=30),正常对照组大鼠不做任何处理,单纯损伤组及VPA治疗组大鼠建立闭合性颅脑损伤模型;VPA治疗组大鼠按每天300 mg·kg-1 的剂量经腹腔注射给药进行干预,单纯损伤组大鼠给予腹腔注射等量的生理盐水进行干预,分别于颅脑损伤后3、7、14、21、28 d断头取脑标本,采用免疫荧光技术检测各组大鼠不同时间点海马齿状回内5-乙炔基-2′-脱氧尿嘧啶核苷(Edu)阳性细胞数及大鼠海马齿状回内巢蛋白(Nestin) 阳性细胞数的变化。结果 单纯损伤组和VPA治疗组大鼠海马齿状回内Edu阳性细胞数及Nestin阳性细胞数于造模后第3天开始增高,于造模后第21天达高峰,在同一时间点单纯损伤组和VPA治疗组大鼠Edu阳性细胞数及Nestin阳性细胞数均高于正常对照组(P<0.01),而VPA治疗组各时间点Edu阳性细胞数及Nestin阳性细胞数较单纯损伤组明显增加(P<0.01)。结论 丙戊酸对颅脑损伤大鼠海马成体NSC有原位激活作用。
Abstract:
Objective To observe the effect of valproic acid (VPA) on the situ activation of hippocampal adut neural stem cells(NSC) in brain tissue of rats with experimental traumatic brain injury.Methods Sixty-six healthy male Sprague-Dawley rats were randomly divided into normal control group(n=6),simple trauma group(n=30) and VPA treated group(n=30).The rats in normal group were not given any thing.Rat model of closed head injury was established in simple trauma group and VPA treated group.The VPA treated group was interfered by intraperitoneal injecting VPA according to the daily dose of 300 mg·kg-1·d-1;the simple trauma group was intraperitoneal injected the same amount of saline to intervene.All the rats were sacrificed by decapitation at 3,7,14,21,28 days after brain injury.Immunofluorescence was employed to detect the changes of 5-ethynyl-2′- deoxyuridine(Edu) positive cells and nest protein(Nestin) in hippocampal dentate gyrus of each rats.Results The Edu and Nestin positive cells number in the hippocampal dentate gyrus of simple trauma group and VPA treated group were rised within 3 days after the damage,and were rised to a peak within 21 days after the damage.At the same time,the number of Edu and Nestin positive cells of the simple trauma group and VPA treated group rats were higher than those of the normal control group;at each time point,the number of Edu and Nestin positive cells increased more significantly in the VPA treated group compared with simple trauma group (P<0.01).Conclusion VPA has situ activation on hippocampal adult NSC in brain tissue of rats with experimental traumatic brain injury.

参考文献/References:

[1] 郭熙雄,张美彪.丙戊酸钠治疗急性脑出血临床疗效观察[J].河北医科大学学报,2013,34(5):519-521.
[2] 檀亚航,张渝,卢永良,等.丙戊酸钠治疗脑缺血机制的研究进展[J].中风与神经疾病杂志,2013,30(10):956-957.
[3] 陈彩霞,黄寅平,吴震卿.丙戊酸钠合并奥氮平治疗阿尔茨海默病精神行为症状临床观察[J].实用老年医学,2009,23(6):432-433,440.
[4] 张玉梅,吴雪飞,于德钦,等.在小鼠帕金森病模型中观察丙戊酸钠对多巴胺神经元及GDNF表达的影响[J].神经解剖学杂志,2011,27(1):1-7.
[5] 田贵华,谭纯,姚海江,等.针刺对大鼠颅脑损伤后轴突导向因子Slit2表达的影响[J].中华中医药杂志,2011,26(5):1197-1200.
[6] 刘胜,王诚,刘远新,等.颅脑损伤急性期灌注压变化后引起兔脑神经元凋亡的研究[J].中华实验外科杂志,2009,26(1):127.
[7] LI S,WEI M,ZHOU Z,et al.SDF-1alpha induces angiogenesis after traumatic brain injury[J].Brain Res,2012,1444:76-86.
[8] 金保哲,李武雄,司昊天,等.丙戊酸对大鼠颅脑损伤局部炎性反应的抑制作用[J].中华实验外科杂志,2014,31(9):1910-1912.
[9] 叶建亚,李聪慧,唐蔚东,等.巢蛋白及性别决定基因高迁移率组蛋白在SAMP8小鼠穹隆下器的表达及意义[J].解剖学报,2014,45(2):176-180.
[10] 林惠,杨万章.丹参注射液对脑梗死大鼠SVZ干细胞增殖作用的研究[J].中西医结合心脑血管病杂志,2014,12(3):350-352.
[11] 王卫东,程毓华,王津存,等.成年大鼠弥漫性脑损伤后海马齿状回神经元再生动态变化的实验研究[J].中国临床康复,2002,6(19):2864-2865.
[12] 李凌云,秦正红,梁中琴.丙戊酸盐的神经保护作用及其机制的研究进展[J].中国药理学通报,2007,23(3):295-298.
[13] AMES C P,SMITH J S,PREUL M C,et al.Effect of recombinant human bone morphogenetic protein-2 in an experimental model of spinal fusion in a radiated area[J].Spine (Phila Pa 1976),2005,30(23):2585-2592.
[14] COURTES S,VERNEREY J,PUJADAS L,et al.Reelin controls progenitor cell migration in the healthy and pathological adult mouse brain[J].PLoS One,2011,6(5):e20430.
[15] 转黎,王涛,李明超,等.氯甲基苯甲酰胺和5-乙炔基-2′-脱氧尿嘧啶核苷标记示踪大鼠脂肪干细胞的研究[J].中华实验外科杂志,2013,30(8):1701-1703.
[16] UCHIDA K,OKANO H,HAYASHI T,et al.Grafted swine neuroepithelial stem cells can form myelinated axons and both efferent and afferent synapses with xenogeneic rat neurons[J].J Neurosci Res,2003,72(6):661-669.
[17] AN Y H,WAN H,ZHANG Z S,et al.Effect of rat Schwann cell secretion on proliferation and differentiation of human neural stem cells[J].Biomed Environ Sci,2003,16(1):90-94.

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更新日期/Last Update: 2016-06-05