[1]谷宁,王鹏辉,王振祥,等.斑蝥酸钠对食管癌EC9706细胞增殖、迁移及侵袭的影响及机制[J].新乡医学院学报,2023,40(12):1114-1120.[doi:10.7683/xxyxyxb.2023.12.003]
　GU Ning,WANG Penghui,WANG Zhenxiang,et al.Effect and mechanism of sodium cantharidate on proliferation,migration and invasion of esophageal cancer EC9706 Cells[J].Journal of Xinxiang Medical University,2023,40(12):1114-1120.[doi:10.7683/xxyxyxb.2023.12.003]
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斑蝥酸钠对食管癌EC9706细胞增殖、迁移及侵袭的影响及机制
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
40卷
期数:
2023年12
页码:
1114-1120
栏目:
基础研究
出版日期:
2023-12-05

文章信息/Info

Title:
Effect and mechanism of sodium cantharidate on proliferation,migration and invasion of esophageal cancer EC9706 Cells
作者:
谷宁1; 王鹏辉2; 王振祥1; 崔凤琴2; 李志刚1
（1.河南中医药大学第三附属医院肿瘤科一病区，河南　郑州　450000;2.河南中医药大学研究生院，河南　郑州　450000）
Author(s):
GU Ning1; WANG Penghui2; WANG Zhenxiang1; CUI Fengqin2; LI Zhigang1
(1.Department of Oncology,the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450000,Henan Province,China;2.Graduate School of Henan University of Traditional Chinese Medicine,Zhengzhou 450000,Henan Province,China)
关键词:
斑蝥酸钠; 食管癌; Wnt3a/β-catenin通路
Keywords:
sodium cantharidate; esophageal cancer; Wnt3a/β-catenin pathway
分类号:
R735.1
DOI:
10.7683/xxyxyxb.2023.12.003
文献标志码:
A
摘要:
目的　探讨斑蝥酸钠对食管癌EC9706细胞增殖、迁移及侵袭的影响及机制。
方法　将食管癌EC9706细胞随机分为空白对照组、低剂量斑蝥酸钠组、中剂量斑蝥酸钠组、高剂量斑蝥酸钠组及顺铂组，低、中、高剂量斑蝥酸钠组EC9706细胞分别给予终质量浓度1.0、2.5、5.0 mg·L-1的斑蝥酸钠干预，顺铂组EC9706细胞给予终质量浓度140 mg·L-1的顺铂干预，空白对照组使用达尔伯克改良伊格尔培养基培养；采用细胞计数试剂-8法检测各组细胞增殖率，划痕实验检测各组EC9706细胞迁移率，Transwell实验检测各组EC9706细胞侵袭率，实时定量聚合酶链式反应法检测各组EC9706细胞中Wnt3a及β-catenin mRNA水平，Western blot法检测各组EC9706细胞中Wnt3a及β-catenin蛋白水平。
结果　培养24、48、72 h，低剂量斑蝥酸钠组、中剂量斑蝥酸钠组、高剂量斑蝥酸钠组和顺铂组EC9706细胞增殖率显著低于空白对照组(P<0.05)；低剂量斑蝥酸钠组、中剂量斑蝥酸钠组EC9706细胞增殖率显著高于高剂量斑蝥酸钠组和顺铂组(P<0.05)；低剂量斑蝥酸钠组EC9706细胞增殖率显著高于中剂量斑蝥酸钠组 (P<0.05)；高剂量斑蝥酸钠组与顺铂组EC9706细胞增殖率比较差异无统计学意义(P>0.05)；低剂量斑蝥酸钠组、中剂量斑蝥酸钠组、高剂量斑蝥酸钠组和顺铂组EC9706细胞增殖率随着培养时间的延长显著降低(P<0.05)。低剂量斑蝥酸钠组、中剂量斑蝥酸钠组、高剂量斑蝥酸钠组和顺铂组EC9706细胞迁移率、侵袭率显著低于空白对照组(P<0.05)；低剂量斑蝥酸钠组、中剂量斑蝥酸钠组EC9706细胞迁移率、侵袭率显著高于高剂量斑蝥酸钠组和顺铂组(P<0.05)；低剂量斑蝥酸钠组EC9706细胞迁移率、侵袭率显著高于中剂量斑蝥酸钠组 (P<0.05)；高剂量斑蝥酸钠组与顺铂组EC9706细胞迁移率、侵袭率比较差异无统计学意义(P>0.05)。低剂量斑蝥酸钠组、中剂量斑蝥酸钠组、高剂量斑蝥酸钠组和顺铂组EC9706细胞中Wnt3a及β-catenin mRNA和蛋白相对表达量显著低于空白对照组(P<0.05)；低剂量斑蝥酸钠组、中剂量斑蝥酸钠组EC9706细胞中Wnt3a及β-catenin mRNA和蛋白相对表达量显著高于高剂量斑蝥酸钠组和顺铂组(P<0.05)；低剂量斑蝥酸钠组EC9706细胞中Wnt3a及β-catenin mRNA和蛋白相对表达量显著高于中剂量斑蝥酸钠组 (P<0.05)；高剂量斑蝥酸钠组与顺铂组EC9706细胞中Wnt3a及β-catenin mRNA和蛋白相对表达量比较差异无统计学意义(P>0.05)。
结论　斑蝥酸钠能够显著抑制食管癌EC9706细胞的增殖、迁移和侵袭能力，其机制可能与抑制Wnt3a/β-catenin通路有关。
Abstract:
Objective　To investigate the effect and mechanism of sodium cantharidate on the proliferation,migration and invasion of esophageal carcinoma EC9706 cells.
Methods　Esophageal cancer EC9706 cells were randomly divided into blank control group,low-dose sodium cantharidate group,medium-dose sodium cantharidate group,high-dose sodium cantharidate group and cisplatin group.The EC9706 cells in the low-,medium- and high-dose sodium cantharidate groups were given final mass concentration of 1.0,2.5 and 5.0 mg·L-1 sodium cantharidate intervention,respectively.The EC9706 cells in the cisplatin group were treated with the final mass concentration of 140 mg·L-1 cisplatin,and the cells in the control group was cultured in Dulbecco′s modified Eagle′s medium.The cell proliferation rate in each group was detected by cell counting reagent-8 method,the mobility of EC9706 cells in each group was detected by scratch test,the invasion rate of EC9706 cells in each group was detected by Transwell method,and the levels of Wnt3a and β-catenin mRNA in EC9706 cells in each group were detected by real-time quantitative polymerase chain reaction method,and the levels of Wnt3a and β-catenin protein in EC9706 cells in each group were detected by Western blot.
Results　At 24,48 and 72 h of cultivation,the proliferation rate of EC9706 cells in the low-dose sodium cantharidate group,medium-dose sodium cantharidate group,high-dose sodium cantharidate group and cisplatin group was significantly lower than that in the blank control group(P<0.05);the proliferation rate of EC9706 cells in the low-dose sodium cantharidate group and medium-dose of sodium cantharidate group was significantly higher than that in the high-dose sodium cantharidate group and cisplatin group(P<0.05);the proliferation rate of EC9706 cells in the low-dose sodium cantharidate group was significantly higher than that in the medium-dose sodium cantharidate group(P<0.05);there was no significant difference in the proliferation rate of EC9706 cells between the high-dose sodium cantharidate group and cisplatin group(P>0.05);the proliferation rate of EC9706 cells in the low-dose sodium cantharidate group,medium-dose sodium cantharidate group,high-dose sodium cantharidate group and cisplatin group was significantly decreased with the extension of culture time(P<0.05).The mobility rate and invasion rate of EC9706 cells in the low-dose sodium cantharidate group,medium-dose sodium cantharidate group,high-dose sodium cantharidate group and cisplatin group were significantly lower than those in the blank control group(P<0.05);the mobility rate and invasion rate of EC9706 cells in the low-dose sodium cantharidate group and medium-dose sodium cantharidate group were significantly higher than those in the high-dose sodium cantharidate group and cisplatin group(P<0.05);the migration rate and invasion rate of EC9706 cells in the low-dose sodium cantharidate group were significantly higher than those in the medium-dose sodium cantharidate group(P<0.05);there was no significant difference in the mobility rate and invasion rate of EC9706 cells between the high-dose sodium cantharidate group and cisplatin group (P>0.05).The relative expression levels of Wnt3a and β-catenin mRNA and protein in EC9706 cells in the low-dose sodium cantharidate group,medium-dose sodium cantharidate group,high-dose sodium cantharidate group and cisplatin group were significantly lower than those in the blank control group(P<0.05);the relative expression levels of Wnt3a and β-catenin mRNA and protein in EC9706 cells in the low-dose sodium cantharidate group and medium-dose sodium cantharidate group were significantly higher than those in the high-dose sodium cantharidate group and cisplatin group(P<0.05);the relative expressions levels of Wnt3a and β-catenin mRNA and protein in EC9706 cells in the low-dose sodium cantharidate group were significantly higher than those in the medium-dose sodium cantharidate group(P<0.05);there was no significant difference in the relative expression levels of Wnt3a and β-catenin mRNA and protein in EC9706 cells between the high-dose sodium cantharidate group and cisplatin group(P>0.05).
Conclusion　Sodium cantharidate can significantly inhibit the proliferation,migration and invasion of esophageal carcinoma EC9706 cells,and its mechanism may be related to the inhibition of the Wnt3a/β-catenin pathway.

参考文献/References:

［1］　BOLLSCHWEILER E,PLUM P,MNIG S P,et al.Current and future treatment options for esophageal cancer in the elderly［J］.Expert Opin Pharmacother,2017,18(10):1001-1010.
［2］　HUANG F L,YU S J.Esophageal cancer:risk factors,genetic association,and treatment［J］.Asian J Surg,2018,41(3):210-215.
［3］　国家卫生健康委员会.食管癌诊疗规范(2018年版)［J］.中华消化病与影像杂志(电子版),2019,9(4):158-192.DOI:10.3877/cma.j.issn.2095-2015.2019.04.005.
NATIONAL HEALTH COMMISSION OF THE PEOPLE′S REPUBLIC OF CHINA.Diagnostic and therapeutic criteria for esophageal cancer (2018 edition)［J］.Chin J Dig Med Imageol(Electr Ed),2019,9(4):158-192.DOI:10.3877/cma.j.issn.2095-2015.2019.04.005.
［4］　侯超,李翔,黄兴广,等.早期食管癌患者内镜黏膜下剥离术后食管狭窄的危险因素及防治方法研究进展［J］.新乡医学院学报,2022,39(3):279-284．
HOU C,LI X,HUANG X G,et al.Research progress on risk factors and prevention methods of endoscopic stricture after endoscopic submucosal dissection in patients with early esophageal carcinoma［J］.J Xinxiang Med Univ,2022,39(3):279-284．
［5］　XU Q L,LI H,ZHU Y J,et al.The treatments and postoperative complications of esophageal cancer:a review［J］.J Cardiothorac Surg,2020,15(1):163.
［6］　樊宏哲,郭浩,杜鹏程.胸腔镜食管癌根治术联合左侧颈部吻合术治疗食管癌疗效观察［J］.新乡医学院学报,2022,39(5):439-444,449．
FAN H Z，GUO H,DU P C.Curative effect of thoracoscopic radical resection of esophageal cancer combined with left neck anastomosis in the treatment of esophageal cancer［J］.J Xinxiang Med Univ,2022,39(5):439-444,449．
［7］　方秀华,徐洁.斑蝥酸钠维生素B6注射液辅助治疗肝癌37例疗效评价［J］.中国药业,2016,25(1):25-27.
FANG X H,XU J.Cantharidin sodium vitamin B6 injection as assistant therapy for treating liver cancer in 37 cases［J］.China Pharm,2016,25(1):25-27.
［8］　邓亚男.斑蝥酸钠联合DCF方案化疗治疗不能手术晚期食管癌患者的疗效分析［J］.首都食品与医药,2021,28(9):77-78.
DENG Y N.Efficacy analysis of sodium cantharidate combined with DCF chemotherapy in the treatment of patients with inoperable advanced esophageal cancer［J］.Cap Food Med,2021,28(9):77-78.
［9］　梁枫,王明艳,许冬青.斑蝥酸钠诱导人食管癌Eca109细胞凋亡实验研究［J］.中国中医药信息杂志,2007,14(3):22-23.
LIANG F,WANG M Y,XU D Q.Experimental study of human esophageal carcinoma cells Eca 109 apoptosis induced by sodium cantharidinate in vitro［J］.Chin J Inf Tradit Chin Med,2007,14(3):22-23.
［10］　刘振宇,黄秀婷,田晓娟,等.Wnt/β-catenin信号通路对氧化应激损伤促进食管癌细胞凋亡的调控研究［J］.广州医科大学学报,2019,47(5):1-4.
LIU Z Y,HUANG X T,TIAN X J,et al.Oxidative stress injury regulates the apoptosis of esophageal cancer cell by Wnt/β-catenin signaling pathway［J］.Acad J Guangzhou Med Univ,2019,47(5):1-4.
［11］　陈宇,袁丽.大蒜素抑制Wnt/β-catenin通路对晚期食管癌中VEGF的调控作用［J］.解剖学研究,2018,40(6):485-488.
CHEN Y,YUAN L.Garlicin inhibits the regulation of VEGF by advanced Wnt/β-catenin pathway in advanced esophageal cancer［J］.Anat Res,2018,40(6):485-488.
［12］　SHORT M W,BURGERS K G,FRY V T.Esophageal cancer［J］.Am Fam Physician,2017,95(1):22-28.
［13］　WATANABE M,OTAKE R,KOZUKI R,et al.Recent progress in multidisciplinary treatment for patients with esophageal cancer［J］.Surg Today,2020,50(1):12-20.
［14］　陈志军,曹克鑫,甘绍印,等.胸腺肽 α1联合顺铂和氟尿嘧啶新辅助化疗治疗食管癌疗效观察［J］.新乡医学院学报,2021,38(3):268-272．
CHEN Z J,CAO K X,GAN S Y,et al.Therapeutic effect of thymosin α1combined with cisplatin and fluorouracil neoadjuvant chemotherapy on esophageal cancer［J］.J Xinxiang Med Univ,2021,38(3): 268-272．
［15］　WANG J,ZHAO Q,CAI L,et al.Efficacy of bevacizumab and gemcitabine in combination with cisplatin in the treatment of esophageal cancer and the effect on the incidence of adverse reactions［J］.Biomed Res Int,2022,2022:2317181.
［16］　梁枫,王明艳,许冬青.斑蝥酸钠对体外培养的人食管癌Eca109细胞中Bcl-2、p53基因表达的影响［J］.时珍国医国药,2010,21(8):2064-2065.
LIANG F,WANG M Y,XU D Q.Effect of sodium cantharidate on the expression of Bcl-2 and p53 genes in human esophageal carcinoma Eca109 cells in vitro［J］.Lishizhen Med Mater Med Res,2010,21(8):2064-2065.
［17］　黄丹仪,吴转斌,黄勤,等.斑蝥酸钠与喜树碱联用抗肿瘤效果的实验研究［J］.中国细胞生物学学报,2014,36(11):1519-1525.
HUANG D Y,WU Z B,HUANG Q,et al.Experimental study on the anti-cancer effect of combined usage of sodium cantharidinate and camptothecin［J］.Chin J Cell Biol,2014,36(11):1519-1525.
［18］　朱文忠,童舟,徐涛.斑蝥酸钠维生素B6联合化疗对肺癌疗效分析［J］.世界中医药,2020,15(11):1607-1610.
ZHU W Z,TONG Z,XU T.Analysis of the efficacy of sodium cantharidin and vitamin B6 combined with chemotherapy on lung cancer［J］.World Chin Med,2020,15(11):1607-1610.
［19］　全昌银,邱小萍,张志强,等.斑蝥酸钠维生素B6治疗晚期乳腺癌的疗效及对生存质量、安全性的影响［J］.中国普通外科杂志,2019,28(5):636-640.
QUAN C Y,QIU X P,ZHANG Z Q,et al.Therapeutic effect of disodium cantharidinate combined with vitamin B6 for advanced stage breast cancer and its influence on quality of life and safety［J］.Chin J Gen Surg,2019,28(5):636-640.
［20］　梁枫,王明艳,许冬青.斑蝥酸钠诱导人胃癌BGC823细胞凋亡的实验研究［J］.南京中医药大学学报,2006,22(3):171-172.
LIANG F,WANG M Y,XU D Q.Experimental study on apoptosis of human gastric cancer BGC823 cells induced by sodium cantharidate［J］.J Nanjing Univ Tradit Chin Med,2006,22(3):171-172.
［21］　刘德仁,朱美玲,张子久,等.斑蝥酸钠注射液结合化疗治疗晚期食管癌疗效观察［J］.中外医学研究,2016,14(31):133-135.
LIU D R,ZHU M L,ZHANG Z J,et al.Efficacy of sodium cantharidate injection combined with chemotherapy in the treatment of advanced esophageal cancer［J］.Chin Foreign Med Res,2016,14(31):133-135.
［22］　蒋淑年,杨帆,马兆明.斑蝥酸钠配合三维适形放疗治疗食管癌近期疗效观察［J］.齐齐哈尔医学院学报,2010,31(20):3258-3259.
JIANG S N,YANG F,MA Z M.Short-term efficacy of sodium cantharidate combined with three-dimensional conformal radiotherapy in the treatment of esophageal cancer［J］.J Qiqihar Med Coll,2010,31(20):3258-3259.
［23］　贾金明,李中华.斑蝥酸钠维生素B6联合静脉化疗治疗晚期食管癌疗效观察［J］.医学信息,2013,26(21):188-189.
JIA J M,LI Z H.Efficacy of sodium cantharidate and vitamin B6 combined with intravenous chemotherapy in the treatment of advanced esophageal cancer［J］.Med Inf,2013,26(21):188-189.
［24］　彭慧,陈丹,李东林,等.MiR-7-5p靶向KLF4基因调控食管癌细胞的增殖和迁移能力［J］.肿瘤,2021,41(4):238-247.
PENG H,CHEN D,LI D L,et al.MiR-7-5p targets KLF4 gene to regulate the proliferation and migration of esophageal cancer cells［J］.Tumor,2021,41(4):238-247.
［25］　王璋,吴泉峰.白藜芦醇调节VEGF/Wnt/β-catenin通路对食管癌细胞的抑制作用［J］.解剖学研究,2018,40(2):108-110,117.
WANG Z,WU Q F.Resveratrol regulates the inhibition of VEGF/Wnt/β-catenin pathway on esophageal cancer cells［J］.Anat Res,2018,40(2):108-110,117.

相似文献/References:

[1]原志庆,王凤荣,吴国祥,等.食管癌切除标本断端残留癌的流式细胞分析[J].新乡医学院学报,1991,8(01):012.
[2]彭明轩,张一峰.食管癌与贲门癌238例手术治疗及其并发症分析[J].新乡医学院学报,1992,9(01):048.
[3]刘玉梅,申维玺,姚卫华,等.氟尿嘧啶合并醛氢叶酸治疗食管癌疗效评价[J].新乡医学院学报,1995,12(02):123.
[4]原志庆,许春雷.瘤体内DNA倍体异质性与食管鳞状细胞癌病理特性的关系[J].新乡医学院学报,1995,12(01):005.
[5]常银江,马森林,王守义,等.上段食管癌切除后食管床内胃食管颈部吻合术[J].新乡医学院学报,1996,13(02):143.
[6]王俊英,李国群,董长宪,等.食管癌合并糖尿病患者的围手术期处理[J].新乡医学院学报,1996,13(02):151.
[7]陈国荣.中晚期食管癌夹心治疗近期疗效分析 [J].新乡医学院学报,1997,14(02):150.
[8]李慧清,李汉臣,杨红伟,等.不同食管胃吻合方法术后生活质量分析[J].新乡医学院学报,1997,14(03):213.
[9]郭玉军,吴阶清,刘新秋,等.双介入疗法治疗晚期食管癌[J].新乡医学院学报,1997,14(03):287.
[10]乔　刚　李汉臣　席红军　王忠民　崔　玉　韩培立　赵宝生.食管癌患者手术前后细胞免疫的变化[J].新乡医学院学报,1999,16(04):308.

更新日期/Last Update: 2023-12-05