[1]李志营,许鑫格,孙林林,等.依达拉奉右莰醇对创伤性颅脑损伤大鼠学习记忆能力及海马区神经细胞凋亡的影响[J].新乡医学院学报,2023,40(3):213-218.[doi:10.7683/xxyxyxb.2023.03.003]
　LI Zhiying,XU Xinge,SUN Linlin,et al.Effect of edaravone dexborneol on learning and memory ability and neuronal apoptosis in hippocampus of rats with traumatic brain injury[J].Journal of Xinxiang Medical University,2023,40(3):213-218.[doi:10.7683/xxyxyxb.2023.03.003]
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依达拉奉右莰醇对创伤性颅脑损伤大鼠学习记忆能力及海马区神经细胞凋亡的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
40卷
期数:
2023年3期
页码:
213-218
栏目:
基础研究
出版日期:
2023-03-05

文章信息/Info

Title:
Effect of edaravone dexborneol on learning and memory ability and neuronal apoptosis in hippocampus of rats with traumatic brain injury
文章编号:
20230303
作者:
李志营1; 许鑫格2; 孙林林3; 武百强4; 王甜甜5; 王艺博6
（1.郑州市第七人民医院医务科，河南　郑州　450016；2.新乡医学院研究生学院，河南　新乡　453003；3.郑州市第七人民医院神经外科，河南　郑州　450016；4.郑州市第七人民医院康复科，河南　郑州　450016；5.郑州市第七人民医院神经内科，河南　郑州　450016；6.郑州市第七人民医院急诊科，河南　郑州　450016）
Author(s):
LI Zhiying1; XU Xinge2; SUN Linlin3; WU Baiqiang4; WANG Tiantian5; WANG Yibo6
(1.Medical Department,the 7th People′s Hospital of Zhengzhou;2.Graduate School,Xinxiang Medical University;3.Department of Neurosurgery,the 7th People′s Hospital of Zhengzhou;4.Department of Rehabilitation,the 7th People′s Hospital of Zhengzhou;5.Department of Neurology,the 7th People′s Hospital of Zhengzhou;6.Department of Emergency,the 7th People′s Hospital of Zhengzhou）
关键词:
颅脑损伤; 细胞凋亡; 依达拉奉右莰醇; 学习记忆能力
Keywords:
craniocerebral injury; cell apoptosis; edaravone dexborneol; learning and memory ability
分类号:
R651.1
DOI:
10.7683/xxyxyxb.2023.03.003
文献标志码:
A
摘要:
目的探讨依达拉奉右莰醇对创伤性颅脑损伤大鼠的学习记忆能力及海马区神经细胞凋亡的影响。
方法将 24只健康成年雄性Sprague-Dawley大鼠随机分为假手术组、颅脑损伤组、药物组,每组8只。颅脑损伤组和药物组大鼠采用Fenny′s自由落体打击法制备颅脑损伤模型,假手术组大鼠不进行自由落体击伤脑部，其他操作同颅脑损伤组和药物组。造模成功后，药物组大鼠给予依达拉奉右莰醇 5 mL·kg-1 腹腔注射，假手术组和颅脑损伤组大鼠给予等体积的生理盐水腹腔注射，均每日1次。造模72 h后，采用穿梭箱试验检测各组大鼠学习记忆能力，分析大鼠的主动逃避反应率及逃避反应时间。采用苏木精-伊红染色法观察各组大鼠海马区神经细胞形态变化，并计数各组大鼠海马区正常及死亡神经细胞。采用原位末端转移酶标记染色法检测各组大鼠海马区神经细胞凋亡情况，计数各组大鼠海马区神经细胞的凋亡细胞及正常细胞。
结果与假手术组比较，颅脑损伤组和药物组大鼠主动逃避反应率降低（P<0.05），逃避反应时间增多（P<0.05）；与颅脑损伤组比较，药物组大鼠主动逃避反应率增高（P<0.05），逃避反应时间减少（P<0.05）。光学显微镜下可见，假手术组大鼠海马区神经细胞的细胞质均一，排列整齐，细胞形态正常，细胞质淡染，细胞核圆形或椭圆形,核仁清晰，染色均匀；颅脑损伤组大鼠海马区神经细胞排列疏松紊乱，细胞质空泡，细胞核出现紫色深染，可见核碎裂，核仁不清，可见大量呈梭形固缩、碎裂溶解细胞,正常形态的神经细胞数量较少；药物组大鼠海马区神经细胞排列稍紊乱，多数神经细胞形态正常,少量神经细胞梭形固缩,少量神经细胞的细胞核深染。与假手术组比较，颅脑损伤组和药物组大鼠海马区正常神经细胞数量显著减少（P<0.05），死亡神经细胞数量显著增多（P<0.05）；与颅脑损伤组比较，药物组大鼠海马区正常神经细胞数量显著增多（P<0.05），死亡神经细胞数量显著减少（P<0.05）。 假手术组大鼠海马区脑组织可见极少量凋亡神经细胞，为浅棕色或棕黄色深染细胞，细胞萎缩及固缩，空泡收缩，细胞结构发生破坏；颅脑损伤组和药物组大鼠海马区神经细胞凋亡细胞数显著多于假手术组（P<0.05），未凋亡细胞数显著少于假手术组（P<0.05）；药物组大鼠海马区神经细胞凋亡细胞数显著少于颅脑损伤组（P<0.05），未凋亡细胞数显著多于颅脑损伤组（P<0.05）。
结论依达拉奉右莰醇可改善颅脑损伤大鼠学习记忆能力，其机制可能与抑制颅脑损伤大鼠海马区神经细胞凋亡有关。
Abstract:
ObjectiveTo explore the effect of edaravone dexborneol on learning and memory ability and neuronal apoptosis in hippocampus of rats with traumatic brain injury.MethodsTwenty-four healthy adult male Sprague-Dawley rats were randomly divided into sham operation group,craniocerebral injury group and drug group,with 8 rats in each group.The rats in the craniocerebral injury group and the drug group were performed with Fenny′s freefall method to prepare the craniocerebral injury model.The rats in the sham operation group were not subjected to free fall injury to the brain，and other operations were same as those in the craniocerebral injury group and the drug group.After successful modeling,the rats in the drug group were injected intraperitoneally with 5 mL· kg-1 of edaravone dexborneol,and the rats in the sham operation group and craniocerebralinjury group was injected intraperitoneally with the same volume of normal saline,once a day.After 72 hours of modeling,the learning and memory ability of rats in each group was tested by shuttle box test,and the active escape reaction rate and escape reaction time of rats were analyzed.The morphological change of neurons in the hippocampus of rats in each group was observed by hematoxylin-eosin staining,and the number of normal and dead neurons in the hippocampus of rats was counted.The apoptosis of neurons in the hippocampus of rats was detected by in situ terminal transferase staining,and the number of apoptotic cells and normal cells in the hippocampus of rats in each group were counted.ResultsCompared with the sham operation group,the active escape reaction rate of rats in the craniocerebral injury group and drug group was significantly decreased (P<0.05),and the escape reaction time was significantly increased (P<0.05);compared with the craniocerebral injury group,the active escape reaction rate of rats in the drug group was significantly increased (P<0.05),and the escape reaction time was significantly decreased (P<0.05).Under the light microscope,the cytoplasm of neurons in the hippocampus of rats in the sham operation group was uniform and orderly arranged,the morphology of neurons was normal,the cytoplasm was light stained,the nucleus was round or oval,the nucleolus was clear,and the staining was uniform;the neurons in the hippocampus of rats in the craniocerebral injury group were arranged loosely and disorderly,with vacuoles in the cytoplasm,purple deep staining in the nucleus,nuclear fragmentation,unclear nucleolus,a large number of spindle-shaped pyknosis,fragmentation and dissolution cells,and a small number of neurons in normal morphology;the arrangement of nerve cells in the hippocampus of rats in the drug group was slightly disordered,most of the nerve cells were normal in morphology,a small number of nerve cells were spindle-shaped pyknosis,and a small number of nerve cells had hyperchromatic nuclei.Compared with the sham operation group,the number of normal nerve cells in the hippocampus of rats in the craniocerebral injury group and drug group was significantly decreased (P<0.05),and the number of dead neurons was significantly increased (P<0.05);comparedwith the craniocerebral injury group,the number of normal nerve cells in the hippocampus of rats in the drug group was significantly increased (P<0.05),and the number of dead neurons was significantly decreased (P<0.05).In the sham operation group,a small number of apoptotic neurons in the hippocampus of rats were found,which were light brown or brownish yellow hyperchromaticcells,with cell atrophy and pyknosis,vacuole contraction,and cell structure destruction.The number of apoptotic neurons in the hippocampus of rats in the craniocerebral injury group and drug group was significantly more than that in the sham operation group,while the number of non-apoptotic cells was significantly less than that in the sham operation group (P<0.05);the number of apoptotic neurons in the hippocampus of rats in the drug group was significantly less than that in the craniocerebral group (P<0.05),while the number of non-apoptotic cells was significantly more than that in the craniocerebral group (P<0.05).ConclusionEdaravone dexborneol can improve the learning and memory ability of rats with brain injury,and its mechanism may be related to the inhibition of neuronal apoptosis in the hippocampus of rats with brain injury.

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更新日期/Last Update: 2023-04-11