[1]姜林娟,郑　志,朱绍辉,等.益生菌植物乳杆菌、鼠李糖乳杆菌、罗伊乳杆菌对医院常见致病病原菌的抑菌作用[J].新乡医学院学报,2022,39(6):501-506.[doi:10.7683/xxyxyxb.2022.06.001]
　JIANG Linjuan,ZHENG Zhi,ZHU Shaohui,et al.Antibacterial effect of Lactobacillus plantarum,Lactobacillus rhamnosus and Lactobacillus reuteri on common pathogenic bacteria in hospital[J].Journal of Xinxiang Medical University,2022,39(6):501-506.[doi:10.7683/xxyxyxb.2022.06.001]
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益生菌植物乳杆菌、鼠李糖乳杆菌、罗伊乳杆菌对医院常见致病病原菌的抑菌作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
39
期数:
2022年6
页码:
501-506
栏目:
基础研究
出版日期:
2022-06-05

文章信息/Info

Title:
Antibacterial effect of Lactobacillus plantarum,Lactobacillus rhamnosus and Lactobacillus reuteri on common pathogenic bacteria in hospital
作者:
姜林娟1; 郑　志1; 2; 朱绍辉3; 侯　栋3; 朴丙熙2; 4; 许旭黎1; 權英珠5
（1．新乡医学院公共卫生学院卫生毒理学教研室,河南　新乡　453003；2.韩陵医药研究院有限公司,江苏　南京　211100；3.新乡医学院第一附属医院普通外科,河南　卫辉　453100；4.莱帕坚公司,韩国　首尔　04393；5.梨花女子大学药学院，韩国　首尔　04393）
Author(s):
JIANG Linjuan1; ZHENG Zhi1; 2; ZHU Shaohui3; HOU Dong3; PARK Byounghee2; 4; XU Xuli1; KWON Youngjoo5
（1.Department of Toxicology,School of Public Health,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;2.Hanling Medical Technology Research Institute Co.,Nanjing 211100,Jiangsu Province,China;3.Department of General Surgery,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China;4.Raphagen Co.,Seoul 04393,Republic of Korea;5.School of Pharmacy,Ewha Woman′s University,Seoul 04393,Republic of Korea）
关键词:
益生菌; 病原菌; 抑菌作用
Keywords:
probiotics; pathogens; antibacterial activity
分类号:
R117，R-33
DOI:
10.7683/xxyxyxb.2022.06.001
文献标志码:
A
摘要:
目的　探讨益生菌植物乳杆菌、鼠李糖乳杆菌、罗伊乳杆菌分别对白色念珠菌、肠炎沙门菌、大肠埃希菌、福氏志贺菌、金黄色葡萄球菌、铜绿假单胞菌6种医院常见致病病原菌的抑菌作用。方法　分别活化3种益生菌和6种病原菌，用无菌生理盐水稀释为1.5×1010 CFU·L-1菌株样品，采用琼脂抑菌试验检测3种益生菌对6种病原菌的抑菌效果。结果　植物乳杆菌对白色念珠菌、大肠埃希菌、福氏志贺菌、金黄色葡萄球菌、铜绿假单胞菌的抑菌率比较差异无统计学意义（F=2.716，P>0.05）；植物乳杆菌对肠炎沙门菌的抑菌率均显著低于白色念珠菌、大肠埃希菌、福氏志贺菌、金黄色葡萄球菌、铜绿假单胞菌（P<0.05）。鼠李糖乳杆菌对白色念珠菌、大肠埃希菌、福氏志贺菌、金黄色葡萄球菌、铜绿假单胞菌的抑菌率比较差异无统计学意义（F=2.400，P>0.05）；鼠李糖乳杆菌对肠炎沙门菌的抑菌率均显著低于白色念珠菌、大肠埃希菌、福氏志贺菌、金黄色葡萄球菌、铜绿假单胞菌（P<0.05）。罗伊乳杆菌对白色念珠菌、大肠埃希菌、福氏志贺菌、金黄色葡萄球菌、铜绿假单胞菌的抑菌率比较差异无统计学意义（F=0.900，P>0.05）；罗伊乳杆菌对肠炎沙门菌的抑菌率均显著低于白色念珠菌、大肠埃希菌、福氏志贺菌、金黄色葡萄球菌、铜绿假单胞菌（P<0.05）。3种益生菌对白色念珠菌、福氏志贺菌、铜绿假单胞菌的抑菌率比较差异无统计学意义（F=1.000、0.000、0.000，P>0.05）。3种益生菌对肠炎沙门菌、大肠埃希菌、金黄色葡萄球菌的抑菌率比较差异有统计学意义（F=3 426.000、111.000、433.000，P<0.05）；罗伊乳杆菌对肠炎沙门菌的抑菌率显著高于植物乳杆菌和鼠李糖乳杆菌（P<0.05）；鼠李糖乳杆菌、罗伊乳杆菌对大肠埃希菌的抑菌率显著高于植物乳杆菌（P<0.05）；罗伊乳杆菌对金黄色葡糖球菌的抑菌率显著高于植物乳杆菌和鼠李糖乳杆菌，鼠李糖乳杆菌对金黄色葡萄球菌的抑菌率显著高于植物乳杆菌（P<0.05）。结论　益生菌植物乳杆菌、鼠李糖乳杆菌、罗伊乳杆菌对白色念珠菌、福氏志贺菌、铜绿假单胞菌、大肠埃希菌、金黄色葡萄球菌的抑菌效果明显，对肠炎沙门菌抑菌效果欠佳。
Abstract:
Objective　To investigate the antibacterial effects of Lactobacillus plantarum,Lactobacillus rhamnosus and Lactobacillus reuteri on six common pathogenic bacteria in hospital including Candida albicans,Salmonella enteritis,Escherichia coli,Shigella flexneri,Staphylococcus aureus and Pseudomonas aeruginosa.Methods　The three kinds of probiotics and six kinds of pathogens were activated respectively,and they were diluted with sterile saline to 1.5 × 1010 CFU·L-1 strain samples.The bacteriostatic effects of three probiotics on the six pathogens were detected by the agar antibacterial test.Results　There was no significant difference in the antibacterial rate of Lactobacillus plantarum to Candida albicans,Escherichia coli,Shigella flexneri,Staphylococcus aureus and Pseudomonas aeruginosa (F=2.716,P>0.05);the antibacterial rate of Lactobacillus plantarum to Salmonella enteritidis was significantly lower than that to Candida albicans,Escherichia coli,Shigella flexneri,Staphylococcus aureus and Pseudomonas aeruginosa (P<0.05).There was no significant difference in the antibacterial rate of Lactobacillus rhamnosus to Candida albicans,Escherichia coli,Shigella flexneri,Staphylococcus aureus and Pseudomonas aeruginosa(F=2.400,P>0.05);the antibacterial rate of Lactobacillus rhamnosus to Salmonella enteritidis was significantly lower than that to Candida albicans,Escherichia coli,Shigella flexneri,Staphylococcus aureus and Pseudomonas aeruginosa(P<0.05).There was no significant difference in the antibacterial rate of Lactobacillus reuteri to Candida albicans,Escherichia coli,Shigella flexneri,Staphylococcus aureus and Pseudomonas aeruginosa(F=0.900,P>0.05);the antibacterial rate of Lactobacillus reuteri to Salmonella enteritidis was significantly lower than that to Candida albicans,Escherichia coli,Shigella flexneri,Staphylococcus aureus and Pseudomonas aeruginosa (P<0.05).There was no significant difference in the antibacterial rates of the three probiotics to Candida albicans,Shigella flexneri and Pseudomonas aeruginosa (F=1.000,0.000,0.000;P>0.05).There was significant difference in the antibacterial rates of the three probiotics to Salmonella enteritis,Escherichia coli,Staphylococcus aureus (F=3 426.000,111.000,433.000;P<0.05);the antibacterial rate of Lactobacillus reuteri to Salmonella enteritidis was significantly higher than that of Lactobacillus plantarum and Lactobacillus rhamnosus to Salmonella enteritidis (P<0.05);the antibacterial rate of Lactobacillus rhamnosus and Lactobacillus reuteri to Escherichia coli was significantly higher than that of Lactobacillus plantarum to Escherichia coli (P<0.05);the antibacterial rate of Lactobacillus reuteri to Staphylococcus aureus was significantly higher than that of Lactobacillus plantarum and Lactobacillus rhamnosus to Staphylococcus aureus,and the antibacterial rate of Lactobacillus rhamnosus to Staphylococcus aureus was significantly higher than that of Lactobacillus plantarum to Staphylococcus aureus (P<0.05).Conclusion　The probiotics of Lactobacillus plantarum,Lactobacillus rhamnosus and Lactobacillus reuteri have significant bacteriostatic effects on Candida albicans,Escherichia coli,Shigella flexneri,Staphylococcus aureus and Pseudomonas aeruginosa,but their antibacterial effect against Salmonella enteritis is not good.

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更新日期/Last Update: 2022-06-05