[1]翁孝琴,薛　山,罗彬彬,等.舍曲林对抑郁症大鼠认知功能和海马突触后致密蛋白-95 mRNA表达的影响[J].新乡医学院学报,2022,39(3):208-213.[doi:10.7683/xxyxyxb.2022.03.002]
　WENG Xiaoqin,XUE Shan,LUO Binbin,et al.Effect of sertraline on cognitive function and the expression of postsynaptic density protein-95 mRNA in hippocampus of depression disorder rats[J].Journal of Xinxiang Medical University,2022,39(3):208-213.[doi:10.7683/xxyxyxb.2022.03.002]
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舍曲林对抑郁症大鼠认知功能和海马突触后致密蛋白-95 mRNA表达的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
39
期数:
2022年3
页码:
208-213
栏目:
基础研究
出版日期:
2022-03-05

文章信息/Info

Title:
Effect of sertraline on cognitive function and the expression of postsynaptic density protein-95 mRNA in hippocampus of depression disorder rats
作者:
翁孝琴1; 薛　山2; 罗彬彬1; 陈佐明1
（1.新乡医学院第二附属医院临床心理科，河南　新乡　453000;2.郑州市第二人民医院眼科，河南　郑州　451100）
Author(s):
WENG Xiaoqin1; XUE Shan2; LUO Binbin1; CHEN Zuoming1
(1.Department of Psychology，the Second Affiliated Hospital of Xinxiang Medical University,Xinxiang 453000，Henan Province,China;2.Department of Ophthalmology,the Second People′s Hospital of Zhengzhou,Zhengzhou 451100,Henan Province,China)
关键词:
抑郁症; 突触后致密蛋白-95; 海马; 舍曲林; 神经可塑性
Keywords:
depression disorder; postsynaptic density protein-95; hippocampus; sertraline; neural plasticity
分类号:
R749.4
DOI:
10.7683/xxyxyxb.2022.03.002
文献标志码:
A
摘要:
目的　探讨舍曲林对抑郁症大鼠认知功能和海马突触后致密蛋白-95（PSD-95）mRNA表达的影响,分析PSD-95 mRNA表达与抑郁症大鼠认知功能之间的关系。方法　在64只成年健康雄性Sprague-Dawle大鼠中随机选择16只作为对照组，余48只大鼠作为模型组，对照组大鼠正常饲养，模型组大鼠给予慢性不可预见应激制备抑郁症模型。造模成功后，将模型组大鼠随机分为抑郁组、抑郁+生理盐水组和抑郁+舍曲林组，每组16只。抑郁+舍曲林组大鼠每日灌胃舍曲林5.8 mg·kg-1，抑郁+生理盐水组大鼠每日灌胃生理盐水5.8 mg·kg-1，均干预4周；抑郁组大鼠不给予任何干预措施。记录并比较对照组和模型组大鼠体质量、蔗糖水摄入量及运动总距离。采用Y迷宫实验和巴恩斯迷宫实验检测对照组、抑郁组、抑郁+生理盐水组和抑郁+舍曲林组大鼠的认知功能，然后处死大鼠,采用反转录聚合酶链反应法检测4组大鼠海马组织中PSD-95 mRNA相对表达量，并分析大鼠海马组织中PSD-95 mRNA相对表达量与认知功能的关系。结果　模型组大鼠体质量、蔗糖水摄入量、运动总距离均少于对照组（P<0.05）。模型组大鼠正确反应次数少于对照组，错误反应次数多于对照组，总潜伏期反应时间长于对照组（P<0.05)。模型组大鼠第2、4天进入目标洞时间长于对照组（P<0.05）；2组大鼠第1、3天进入目标洞时间比较差异无统计学意义（P>0.05）。抑郁组、抑郁+生理盐水组大鼠正确反应次数少于对照组，错误反应次数多于对照组，总潜伏期反应时间长于对照组（P<0.05）。抑郁+舍曲林组与对照组大鼠正确反应次数、错误反应次数、总潜伏期反应时间比较差异无统计学意义（P>0.05）。抑郁+舍曲林组大鼠正确反应次数多于抑郁组和抑郁+生理盐水组，错误反应次数少于抑郁组和抑郁+生理盐水组，总潜伏期反应时间短于抑郁组和抑郁+生理盐水组（P<0.05）。抑郁组与抑郁+生理盐水组大鼠正确反应次数、错误反应次数和总潜伏期反应时间比较差异无统计学意义（P>0.05）。抑郁组、抑郁+生理盐水组大鼠第1、2、3、4天进入目标洞时间均长于对照组（P<0.05）。抑郁+舍曲林组与对照组大鼠第1、2、3、4天进入目标洞时间比较差异无统计学意义（P>0.05）。抑郁+舍曲林组大鼠第1、2、3天进入目标洞时间短于抑郁组和抑郁+生理盐水组（P<0.05）；第4天进入目标洞时间与抑郁组和抑郁组+生理盐水组比较差异无统计学意义（P>0.05）。抑郁组与抑郁+生理盐水组大鼠第1、2、3、4天进入目标洞时间比较差异无统计学意义（P>0.05）。抑郁组、抑郁+生理盐水组大鼠海马组织中PSD-95 mRNA相对表达量低于对照组（P<0.05）。抑郁+舍曲林组与对照组大鼠海马组织中PSD-95 mRNA相对表达量比较差异无统计学意义（P>0.05）。抑郁+舍曲林组大鼠海马组织中PSD-95 mRNA 相对表达量高于抑郁组和抑郁+生理盐水组（P<0.05）。抑郁组与抑郁+生理盐水组大鼠海马组织中PSD-95 mRNA相对表达量比较差异无统计学意义（P>0.05）。大鼠海马组织中PSD-95 mRNA表达水平与大鼠在Y迷宫实验中正确反应次数呈显著正相关（r=0.486,P<0.05）,与错误反应次数呈显著负相关（r=-0.581,P<0.05）。结论　舍曲林能改善抑郁症大鼠的认知功能,增加海马组织中PSD-95 mRNA的表达量，大鼠海马组织中PSD-95 mRNA的表达与大鼠认知功能呈正相关。
Abstract:
Objective　To investigate the effect of sertraline on the cognitive function and the expression of postsynaptic density protein-95 (PSD-95) mRNA in the hippocampus of depression disorder rats,and analyze the relationship between the expression of PSD-95 mRNA and the cognitive function of depression disorder rats.Methods　Among 64 healthy adult male Sprague-Dawle rats,16 rats were randomly selected as the control group and the other 48 rats were as the model group.The rats in the control group were fed normally,and the rats in the model group were given chronic unpredictable stress to made the depression disorder model.After successful modeling,the rats in the model group were randomly divided into depression group,depression+normal saline group and depression+sertraline group,with 16 rats in the each group.The rats in depression+sertraline group were given sertraline 5.8 mg·kg-1 daily and the rats in depression+saline group were given normal saline 5.8 mg·kg-1 daily by intragastric administration for 4 weeks;the rats in depression group were not given any intervention measures.The body mass,sucrose water intake and total exercise distance of rats in the control group and the model group were recorded and compared.The cognitive function of rats in the control group,depression group,depression+normal saline group and depression+sertraline group was detected by Y maze test and Barnes maze test,and then the rats were sacrificed.The relative expression of PSD-95 mRNA in the hippocampus of rats in the four groups was detected by reverse transcription-polymerase chain reaction,and the relationship between the relative expression of PSD-95 mRNA and the cognitive function of rats was analyzed.Results　The body mass,sucrose water intake and total exercise distance of rats in the model group were less than those in the control group (P<0.05).The number of correct reactions of rats in the model group was less than that in the control group,and the number of wrong reactions and the total latency reaction time in the model group were more than those in the control group (P<0.05).The time of entering the target hole of rats in the model group was longer than that in the control group on the 2nd day and the 4th day (P<0.05);there was no significant difference in the time of entering the target hole between the two groups on the 1st day and the 3rd day (P>0.05).The number of correct reactions of rats in the depression group and depression+normal saline group was less than that in the control group (P<0.05),the number of wrong reaction and total latency reaction time were more than those in the control group (P<0.05).There was no significant difference in the number of correct reaction,the number of wrong reaction and the total latency reaction time between the depression+sertraline group and the control group (P>0.05).The number of correct reaction of rats in the depression+sertraline group was more than that in the depression group and depression+normal saline group (P<0.05),and the number of wrong reaction and the total latency reaction time were less than those in the depression group and depression+normal saline group (P<0.05).There was no significant difference in the number of correct reaction,the number of wrong reaction and the total latency reaction time of rats between the depression group and the depression+saline group (P>0.05).The time of entering the target hole of rats in the depression group and depression+normal saline group was longer than that in the control group on the 1st,2nd,3rd and 4th day(P<0.05).There was no significant difference in the time of entering the target hole of rats between the depression+sertraline group and the control group on the 1st,2nd,3rd and 4th day (P>0.05).The time of entering the target hole of rats in the depression+sertraline group on the 1st,2nd and 3rd day was less than that in the depression group and the depression+normal saline group (P<0.05);there was no significant difference in the time of entering the target hole on the 4th day between the depression+sertraline group and the depression group,the depression+normal saline group (P>0.05).There was no significant difference in the time of entering the target hole of rats between the depression group and the depression+normal saline group on the 1st,2nd,3rd and 4th day (P>0.05).The relative expression of PSD-95 mRNA in hippocampus of rats in the depression group and depression+normal saline group was less than that in the control group (P<0.05).There was no significant difference in the relative expression of PSD-95 mRNA in hippocampus of rats between the depression+sertraline group and the control group (P>0.05).The relative expression of PSD-95 mRNA in hippocampus of rats in depression+sertraline group was higher than that in the depression group and depression+normal saline group (P< 0.05).There was no significant difference in the relative expression of PSD-95 mRNA in hippocampus of rats between the depression group and the depression+normal saline group (P>0.05).The expression of PSD-95 mRNA in hippocampus of rats was positively correlated with the number of correct reaction (r= 0.486,P<0.05) and was negatively correlated with the number of wrong reaction (r=-0.581,P<0.05).Conclusion　Sertraline can improve the cognitive function of depression rats and increase the expression of PSD-95 mRNA in hippocampus.The expression of PSD-95 mRNA in hippocampus is positively correlated with the cognitive function of rats.

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更新日期/Last Update: 2022-03-05