[1]张　澍,王春延,忽　平,等.子痫前期患者胎盘组织中微RNA-210和Foxp3的表达及临床意义[J].新乡医学院学报,2022,39(2):165-169.[doi:10.7683/xxyxyxb.2022.02.013]
　ZHANG Shu,WANG Chunyan,HU Ping,et al.Expression and clinical significance of microRNA-210 and Foxp3 in placental tissues of patients with preeclampsia[J].Journal of Xinxiang Medical University,2022,39(2):165-169.[doi:10.7683/xxyxyxb.2022.02.013]
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子痫前期患者胎盘组织中微RNA-210和Foxp3的表达及临床意义
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
39
期数:
2022年2
页码:
165-169
栏目:
临床研究
出版日期:
2022-02-05

文章信息/Info

Title:
Expression and clinical significance of microRNA-210 and Foxp3 in placental tissues of patients with preeclampsia
作者:
张　澍; 王春延; 忽　平; 姚　利
（南阳市中心医院/郑州大学附属南阳中心医院妇产科，河南　南阳　473000）
Author(s):
ZHANG Shu; WANG Chunyan; HU Ping; YAO Li
(Department of Gynaecology and Obstetrics,Nanyang Central Hospital/Nanyang Central Hospital Affiliated to Zhengzhou University,Nanyang 473000,Henan Province,China)
关键词:
子痫前期; 微RNA-210; Foxp3
Keywords:
preeclampsia; microRNA-210; Foxp3
分类号:
R714.244
DOI:
10.7683/xxyxyxb.2022.02.013
文献标志码:
A
摘要:
目的　探讨子痫前期（PE）患者胎盘组织中微RNA（miR）-210、Foxp3的表达及临床意义。方法　选择2018年6月至2019年12月南阳市中心医院收治的且以剖宫产方式分娩的36例PE患者为PE组，选择同期正常妊娠孕妇35例为正常妊娠组，另选择体检健康的未妊娠女性40例为未妊娠组。采用流式细胞术检测3组受试者外周静脉血CD4+CD25+CD127-调节性T细胞（Treg细胞）、CD4+辅助性T淋巴细胞（Th细胞）水平，计算CD4+CD25+CD127-Treg细胞与CD4+Th细胞的比值（CD4+CD25+CD127-/CD4+）；采用酶联免疫吸附试验检测3组受试者血清白细胞介素（IL）-6、IL-10、IL-17水平；实时荧光定量聚合酶链反应法检测PE组和正常妊娠组受试者胎盘组织中Foxp3 mRNA、miR-210表达，Western blot法检测胎盘组织中Foxp3蛋白表达。结果　PE组、正常妊娠组与未妊娠组受试者外周静脉血CD4+CD25+CD127-/CD4+分别为（6.72±0.31）%、（8.92±0.37）%、（8.85±0.34）%；PE组受试者外周静脉血CD4+CD25+CD127-/CD4+显著低于正常妊娠组和未妊娠组（t=27.136、28.581，P<0.05）；正常妊娠组与未妊娠组受试者外周静脉血CD4+CD25+CD127-/CD4+比较差异无统计学意义（t=0.849，P>0.05）。3组受试者血清IL-6、IL-10、IL-17水平比较差异有统计学意义（F=53.584、133.925、42.392，P<0.05）。PE组受试者血清IL-6水平显著高于正常妊娠组（t=9.170，P<0.05），正常妊娠组受试者血清IL-6水平显著低于未妊娠组（t=8.844，P<0.05）；PE组与未妊娠组受试者血清IL-6水平比较差异无统计学意义（t=1.608，P>0.05）。PE组受试者血清IL-10水平显著低于正常妊娠组和未妊娠组（t=14.929、4.892，P<0.05），正常妊娠组受试者血清IL-10水平显著高于未妊娠组（t=10.914，P<0.05）。PE组受试者血清IL-17水平显著高于正常妊娠组和未妊娠组（t=8.184、3.038，P<0.05），正常妊娠组受试者血清IL-17水平显著低于未妊娠组（t=7.325，P<0.05）。PE组受试者胎盘组织中Foxp3 mRNA相对表达量显著低于正常妊娠组（t=13.948，P<0.05），miR-210相对表达量显著高于正常妊娠组（t=16.565，P<0.05）。PE组和正常妊娠组受试者胎盘组织中Foxp3蛋白相对表达量分别为0.97±0.11、1.38±0.18，PE组受试者胎盘组织中Foxp3蛋白相对表达量显著低于正常妊娠组（t=11.625，P<0.05）。结论　PE患者外周静脉血Treg细胞水平显著降低，Treg/Th17细胞失衡，可破坏母体对胎儿的免疫耐受；PE患者胎盘组织中Foxp3表达水平下降可能与miR-210表达上调有关。
Abstract:
Objective　To investigate the expression and clinical significance of microRNA(miR)-210 and Foxp3 in placental tissues of patients with preeclampsia (PE).Methods　Thirty-six PE patients who underwent cesarean section in Nanyang Central Hospital from June 2018 to December 2019 were selected as the PE group,thirty-five normal pregnancy pregnant women during the same period were selected as the normal pregnancy group,and 40 healthy non-pregnant women were selected as the non-pregnant group.The levels of CD4+CD25+CD127- regulatory T cells (Treg cell) and CD4+ helper T lymphocytes (Th cell) in peripheral venous blood of the subjects in the three groups were detected by flow cytometry,and the ratio of CD4+ CD25+CD127-Treg cells to CD4+ Th cells (CD4+CD25+CD127-/CD4+) was calculated.The levels of serum interleukin (IL)-6,IL-10 and IL-17 of the subjects in the three groups were detected by enzyme-linked immunosorbent assay.The expressions of Foxp3 mRNA and miR-210 in placental tissues of the subjects in the PE group and normal pregnancy group were detected by real-time fluorescence quantitative polymerase chain reaction,and the expression of Foxp3 protein in placental tissues was detected by Western blot.Results　The CD4+CD25+CD127-/CD4+ in peripheral venous blood of subjects in the PE group,normal pregnancy group and non-pregnancy group was (6.72±0.31)%,(8.92±0.37)% and (8.85±0.34)%,respectively.The CD4+CD25+CD127-/CD4+ in peripheral venous blood of subjects in the PE group was significantly lower than that in the normal pregnancy group and non-pregnancy group (t=27.136,28.581;P<0.05).There was no significant difference in the CD4+CD25+CD127-/CD4+ in peripheral venous blood of subjects between the normal pregnancy group and non-pregnancy group (t=0.849,P>0.05).There was significant difference in the levels of serum IL-6,IL-10 and IL-17 among the three groups (F=53.584,133.925,42.392;P<0.05).The level of serum IL-6 in the PE group was significantly higher than that in the normal pregnancy group (t=9.170,P<0.05),and the level of serum IL-6 in the normal pregnancy group was significantly lower than that in the non-pregnancy group (t=8.844,P<0.05),but there was no significant difference in the level of serum IL-6 between the PE group and non-pregnant group (t=1.608,P>0.05).The level of serum IL-10 in the PE group was significantly lower than that in the normal pregnancy group and non-pregnancy group (t=14.929,4.892;P<0.05),and the level of serum IL-10 in the normal pregnancy group was significantly higher than that in the non-pregnancy group (t=10.914,P<0.05).The level of serum IL-17 in the PE group was significantly higher than that in the normal pregnancy group and non-pregnancy group (t=8.184,3.038;P<0.05),and the level of serum IL-17 in the normal pregnancy group was significantly lower than that in the non-pregnancy group (t=7.325,P<0.05).The relative expression of Foxp3 mRNA in placental tissues of subjects in the PE group was significantly lower than that in the normal pregnancy group (t=13.948,P<0.05),and the relative expression of miR-210 in placental tissues of subjects in the PE group was significantly higher than that in the normal pregnancy group (t=16.565,P<0.05).The relative expression of Foxp3 protein in placental tissues of subjects in the PE group and normal pregnancy group was 0.97±0.11 and 1.38±0.18,respectively.The relative expression of Foxp3 protein in placental tissues of subjects in the PE group was significantly lower than that in the normal pregnancy group (t=11.625,P<0.05).Conclusion　The level of Treg cells in peripheral venous blood of PE patients is significantly decreased,and the Treg/Th17 cell is unbalanced,which can destroy maternal immune tolerance to the fetus.The decrease of Foxp3 expression in placental tissues of PE patients may be related to the up-regulation of miR-210 expression.

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更新日期/Last Update: 2022-02-05