[1]刘　芳,章龙珍.宫颈癌组织中血管内皮生长因子和Grb2协同结合蛋白2的表达与患者放射治疗敏感性及预后的相关性[J].新乡医学院学报,2022,39(1):051-54.[doi:10.7683/xxyxyxb.2022.01.011]
　LIU Fang,ZHANG Longzhen.Correlation between the expression of vascular endothelial growth factor and Grb2-associated binding protein-2 and radiotherapeutic sensitivity and prognosis of patients with cervical cancer[J].Journal of Xinxiang Medical University,2022,39(1):051-54.[doi:10.7683/xxyxyxb.2022.01.011]
点击复制

宫颈癌组织中血管内皮生长因子和Grb2协同结合蛋白2的表达与患者放射治疗敏感性及预后的相关性
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
39
期数:
2022年1
页码:
051-54
栏目:
临床研究
出版日期:
2022-01-05

文章信息/Info

Title:
Correlation between the expression of vascular endothelial growth factor and Grb2-associated binding protein-2 and radiotherapeutic sensitivity and prognosis of patients with cervical cancer
作者:
刘　芳1; 2; 章龙珍3
（1.徐州医科大学徐州临床学院，江苏　徐州　221009；2.徐州市中心医院病理科，江苏　徐州　221009；3.徐州医科大学附属医院放射治疗科，江苏　徐州　221002）
Author(s):
LIU Fang1; 2; ZHANG Longzhen3
(1.Xuzhou Clinical College of Xuzhou Medical University,Xuzhou 221009,Jiangsu Province,China2.Department of Pathology,Xuzhou Central Hospital,Xuzhou 221009,Jiangsu Province,China3.Department of Radiotherapy,the Affiliated Hospital of Xuzhou Medical Univ
关键词:
宫颈癌; 血管内皮生长因子; Grb2协同结合蛋白2; 放射治疗
Keywords:
cervical cancervascular endothelial growth factorGrb2-associated binding protein-2radiotherapy
分类号:
R737.33
DOI:
10.7683/xxyxyxb.2022.01.011
文献标志码:
A
摘要:
目的　探讨血管内皮生长因子（VEGF）和Grb2协同结合蛋白2（Gab2）在宫颈癌组织中的表达与患者放射治疗敏感性及预后的相关性。方法　选择2014年3月至2015年9月于徐州医科大学附属医院行根治性放疗的120例宫颈癌患者为研究对象，所有患者于放射治疗前取宫颈癌组织，采用实时荧光定量聚合酶链反应法检测宫颈癌组织中VEGF、Gab2 mRNA的表达，采用免疫组织化学法检测宫颈癌组织中VEGF、Gab2蛋白的表达，采用Spearman相关性分析观察VEGF和Gab2蛋白表达水平与放射治疗敏感性的关系；所有患者随访5 a，统计VEGF、Gab2蛋白表达阳性和阴性患者的5 a生存率。结果　放射治疗后3个月，120例患者中，88例肿瘤消失（肿瘤消失组），32例肿瘤未控（肿瘤未控组）；肿瘤消失组患者宫颈癌组织中VEGF和Gab2 mRNA相对表达量显著低于肿瘤未控组（P<0.05）。放射治疗后5 a，肿瘤消失组患者中，53例肿瘤治愈（肿瘤治愈组），35例肿瘤复发（肿瘤复发组）；肿瘤治愈组患者宫颈癌组织中VEGF和Gab2 mRNA相对表达量显著低于肿瘤复发组（P<0.05）。Spearman相关性分析结果显示，宫颈癌组织中VEGF和Gab2 mRNA表达水平与放射治疗敏感性呈显著负相关（r=-0.459、-0.720，P<0.05）。120例患者中，VEGF蛋白表达阳性80例（66.67%），阴性40例（33.33%）；Gab2蛋白表达阳性103例（85.83%），阴性17例（14.17%）。VEGF蛋白表达阳性和阴性患者的5 a生存率分别为16.25%（13/80）、67.5%（27/40），VEGF蛋白表达阳性患者的5 a生存率显著低于表达阴性患者（χ2=31.519，P<0.05）。Gab2蛋白表达阳性和阴性患者的5 a生存率分别为26.21%（27/103）、76.47%（13/17），Gab2蛋白表达阳性患者的5 a生存率低于表达阴性患者（χ2=16.585，P<0.05）。结论　宫颈癌组织中VEGF和Gab2基因表达上调，VEGF和Gab2基因表达水平与放射治疗敏感性呈显著负相关；VEGF和Gab2蛋白表达阴性患者的预后较好，检测VEGF和Gab2蛋白表达对评估宫颈癌患者预后有一定价值。
Abstract:
Objective　To investigate the correlation between the expression of vascular endothelial growth factor（VEGF） and Grb2-associated binding protein-2（Gab2） and radiotherapeutic sensitivity and prognosis of patients with cervical cancer.Methods　A total of 120 patients with cervical cancer who underwent radical radiotherapy in the Affiliated Hospital of Xuzhou Medical University from March 2014 to September 2015 were selected as the research subjects.The cervical cancer tissues were taken from all patients before radiotherapy.The expression of VEGF and Gab2 mRNA in cervical cancer tissues was detected by real-time fluorescence quantitative polymerase chain reaction,and the expression of VEGF and Gab2 protein in cervical cancer tissues was detected by immunohistochemistry.The relationship between the expression of VEGF and Gab2 protein and radiotherapy sensitivity was analyzed by Spearman correlation.All patients were followed up for 5 years,the 5-year survival rate of patients with positive and negative expression of VEGF and Gab2 protein were counted.Results　Three months after radiotherapy,among the 120 patients,the tumors disappeared in 88 cases (tumor disappearance group),and the tumors were not controlled in 32 cases (tumor uncontrolled group).The relative expression of VEGF and Gab2 mRNA in cervical cancer tissues of patients in the tumor disappeared group was significantly lower than that in the tumor uncontrolled group (P<0.05).Five years after radiotherapy,among the patients of tumor disappearance group,53 cases were cured (tumor cure group) and 35 cases recurred (tumor recurrence group).The relative expression of VEGF and Gab2 mRNA in cervical cancer tissues in the tumor cure group was significantly lower than that in the tumor recurrence group (P<0.05).Spearman correlation analysis showed that the expression levels of VEGF and Gab2 mRNA in cervical cancer tissues were significantly negatively correlated with radiotherapy sensitivity (r=-0.459,-0.720P<0.05).Among the 120 patients,the VEGF protein expression was positive in 80 cases (66.67%) and negative in 40 cases (33.33%),Gab2 protein expression was positive in 103 cases (85.83%) and negative in 17 cases (14.17%).The 5-year survival rate of patients with positive and negative expression of VEGF protein was 16.25% (13/80) and 67.50% (27/40),respectively.The 5-year survival rate of patients with positive expression of VEGF protein was significantly lower than that of patients with negative expression of VEGF protein(χ2=31.519,P<0.05).The 5-year survival rate of patients with positive and negative expression of Gab2 protein was 26.21%(27/103) and 76.47%(13/17),respectively.The 5-year survival rate of patients with positive expression of Gab2 protein was significantly lower than that of patients with negative expression of Gab2 protein(χ2=16.585,P<0.05).Conclusion　The expression of VEGF and Gab2 genes in cervical cancer tissues was upregulated,and the expression levels of VEGF and Gab2 genes were significantly negatively correlated with radiosensitivity.The prognosis of patients with negative expression of VEGF and Gab2 protein is better.Detecting the expression of VEGF and Gab2 protein has a certain value in evaluating the prognosis of patients with cervical cancer.

参考文献/References:

［1］　YAMAMOTO K,NAGAO S,SUZUKI K,et al.Pelvic fractures after definitive and postoperative radiotherapy for cervical cancer:a retrospective analysis of risk factors［J］.Gynecol Oncol,2017,147(3):585-588.
［2］　ZHOU Y,WANG W,WEI R,et al.Serum bradykinin levels as a diagnostic marker in cervical cancer with a potential mechanism to promote VEGF expression via BDKRB2［J］.Int J Oncol,2019,55(1):131.
［3］　AL-HUSSEINAWI E,BUI M M,AHMED A A.Grb2-associated binding protein-1 as a biomarker in bone and soft tissue sarcomas［J］.Pathology,2019,51(6):610-614.
［4］　林仲秋.《FIGO 2015妇癌报告》解读连载一：宫颈癌诊治指南解读［J］.中国实用妇科与产科杂志，2015，31（11）：981-985.
LIN Z Q.Interpretation serial 1 of FIGO Women′s Cancer Report 2015:interpretation of guidelines for diagnosis and treatment of cervical cancer［J］.Chin J Pract Gynecol Obstet,2015,31(11):981-985.

相似文献/References:

[1]路平,梁秋冬,郑全庆.宫颈鳞癌中erbB3、erbB4基因蛋白表达与细胞凋亡和增殖的相关性研究[J].新乡医学院学报,2003,20(05):317.
[2]李　娜,李继煜,张景航,等.食管鳞癌中CD44H、P - 选择素和血管内皮生长因子的表达与淋巴结转移的关系[J].新乡医学院学报,2006,23(05):467.
[3]冯书营,李三强,刘小转,等.血管内皮生长因子在四氯化碳诱导的急性肝损伤小鼠肝组织中的表达[J].新乡医学院学报,2011,28(03):268.
[4]张 慧,张 婷,宋 宇,等.薏苡仁对实验性腹腔粘连大鼠腹腔液炎性相关因子及粘连组织血管内皮生长因子、血管内皮标志物CD34表达的影响[J].新乡医学院学报,2014,31(10):792.[doi:10.7683/xxyxyxb.2014.10.006]
[5]严 浩,蒋 蕾,刘 力.人乳头瘤病毒16/18、Bcl-2和 Survivin在宫颈癌组织中的表达[J].新乡医学院学报,2014,31(11):943.[doi:10.7683/xxyxyxb.2014.11.023]
[6]陈广理１,龚树生２,陈 沛３,等.血管内皮生长因子及其双受体系统在鼻咽癌中的表达[J].新乡医学院学报,2014,31(12):974.[doi:10.7683/xxyxyxb.2014.12.003]
[7]刘月君.基质金属蛋白酶９、基质金属蛋白酶组织抑制剂１和血管内皮生长因子在视网膜母细胞瘤组织中的表达[J].新乡医学院学报,2014,31(12):1015.[doi:10.7683/xxyxyxb.2014.12.014]
[8]徐静纯,王聪.长链非编码 RNA 生长停滞特异性转录因子5在宫颈癌中的作用研究进展[J].新乡医学院学报,2022,39(12):1196.[doi:10.7683/xxyxyxb.2022.12.019]
　XU Jingchun,WANG Cong.Research progress on the role of long chain non-coding RNA growth arrest special 5 in cervical cancer[J].Journal of Xinxiang Medical University,2022,39(1):1196.[doi:10.7683/xxyxyxb.2022.12.019]
[9]尚俊伟.宫颈癌及癌前病变相关危险因素分析[J].新乡医学院学报,2015,32(12):1135.
[10]曾松柏,张　梅,董化江,等.姜黄素对急性心肌梗死大鼠血管内皮生长因子及肿瘤坏死因子-α的影响[J].新乡医学院学报,2016,33(10):852.[doi:10.7683/xxyxyxb.2016.10.004]
　ZENG Song-bai,ZHANG Mei,DONG Hua-jiang,et al.Effect of curcumin on vascular endothelial growth factor and tumor necrosis factor-α in rats with acute myocardial infarction[J].Journal of Xinxiang Medical University,2016,33(1):852.[doi:10.7683/xxyxyxb.2016.10.004]

更新日期/Last Update: 2022-01-05