[1]刘福泉,郝丽娇,王　维,等.不同强度运动对高脂饮食大鼠窦房结及心功能的保护作用[J].新乡医学院学报,2021,38(10):911-916.[doi:10.7683/xxyxyxb.2021.10.003]
　LIU Fuquan,HAO Lijiao,WANG Wei,et al.Protective effect of different intensity exercises on sinus node and cardiac function of rats fed with a high-fat diet[J].Journal of Xinxiang Medical University,2021,38(10):911-916.[doi:10.7683/xxyxyxb.2021.10.003]
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不同强度运动对高脂饮食大鼠窦房结及心功能的保护作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
38
期数:
2021年10
页码:
911-916
栏目:
基础研究
出版日期:
2021-10-05

文章信息/Info

Title:
Protective effect of different intensity exercises on sinus node and cardiac function of rats fed with a high-fat diet
作者:
刘福泉1; 郝丽娇2; 王　维1; 柳　净1; 刘　尊1; 董珊珊1
（1.沧州医学高等专科学校健康管理与服务系，河北　沧州　061001；2.沧州市妇幼保健院手术室，河北　沧州　061001）
Author(s):
LIU Fuquan1; HAO Lijiao2; WANG Wei1; LIU Jing1; LIU Zun1; DONG Shanshan1
（1.Department of Health Management and Service,Cangzhou Medical College,Cangzhou 061001,Hebei Province,China;2.Operating Room,Cangzhou Maternal and Child Health Hospital,Cangzhou 061001,Hebei Province,China）
关键词:
高脂饮食; 不同强度运动; 窦房结; 心功能
Keywords:
high fat diet; different intensity exercises; sinoatrial node; cardiac function
分类号:
R541
DOI:
10.7683/xxyxyxb.2021.10.003
文献标志码:
A
摘要:
目的　探讨不同强度运动对高脂饮食大鼠窦房结及心功能的影响。方法　将24只8周龄雄性Sprague Dawley大鼠随机分为A、B、C、D组,每组6只。A组大鼠给予普通饲料饲养，B组大鼠给予高脂饲料饲养，2组大鼠均笼内饲养不运动；C组大鼠给予高脂饲料饲养，同时进行8周无负重游泳运动，每日60 min，每周6 d；D组大鼠给予高脂饲料饲养，同时进行8周高强度（5%体质量负重）游泳运动，每日60 min，每周6 d。末次游泳训练24 h后，应用彩色多普勒超声诊疗仪测试大鼠左心室收缩末期内径（LVDS）、左心室舒张末期内径（LVDD）、左心室收缩末期容积（LVVS）、每搏输出量（SV）、左心室射血分数（LVEF）、左心室缩短分数（LVSF）等心功能指标；大鼠用体积分数10%水合氯醛麻醉后，开胸取窦房结，采用苏木精-伊红染色观察窦房结形态结构变化，油红O染色法测定窦房结脂肪细胞分布及脂肪细胞阳性表达量，免疫组织化学法测定窦房结神经标志物蛋白基因产物9.5（PGP 9.5）表达。结果　B组大鼠LVDS、LVDD、LVVS显著高于A组（P＜0.05），SV、LVEF、LVSF显著低于A组（P＜0.05）。 C组大鼠LVDS、LVDD、LVVS、SV、LVEF与A组比较差异无统计学意义（P>0.05），LVSF显著高于A组（P＜0.05）。C组大鼠LVDS、LVDD、LVVS显著低于B组，SV、LVEF、LVSF显著高于B组（P＜0.05）。D组大鼠SV显著低于C组，LVSF显著高于A组，LVEF、LVSF显著高于B组，SV显著低于C组（P＜0.05）；D组大鼠其余心功能指标分别与A组、B组及C组比较差异均无统计学意义（P>0.05）。与A组大鼠比较，B组大鼠窦房结结构缺失，细胞间隙明显增宽、肌纤维排列混乱；C组大鼠窦房结形态结构良好，细胞间隙均匀较窄，细胞质染色均匀；与C组相比，D组大鼠窦房结形态结构欠佳，细胞间隙略增宽。B组、C组、D组大鼠窦房结区脂肪细胞阳性表达量显著高于A组，B组大鼠窦房结区脂肪细胞阳性表达量显著高于C组和D组，D组大鼠窦房结区脂肪细胞阳性表达量显著高于C组（P＜0.05）。A组大鼠窦房结区PGP 9.5阳性表达量显著高于B组和D组（P＜0.05），C组和D组大鼠窦房结区PGP 9.5阳性表达量显著高于B组（P＜0.05），C组大鼠窦房结区PGP 9.5阳性表达量显著高于D组（P＜0.05）。结论　长期高脂饮食可导致大鼠窦房结区脂肪过度浸润及神经萎缩，导致心功能下降；长期有规律的运动能够通过抑制窦房结脂肪浸润及使神经重塑等机制对高脂饮食大鼠窦房结及心功能发挥保护作用，且有氧运动效果好于大强度运动。
Abstract:
Objective　To investigate the protective effect of different intensity exercises on sinus node and cardiac function in rats fed with a high-fat diet.Methods　Twenty-four 8-week-old male SD rats were randomly divided into group A,group B,group C and group D,with 6 rats in each group.The rats in group A were fed with normal diet,the rats in group B were fed with high-fat diet,and rats in the both groups were kept in cages without exercise;the rats in group C were fed with high-fat diet and performed with swimming without weight-bearing for eight weeks,60 minutes once day for six days in one week;the rats in group D were fed with high-fat diet and performed with high intensity swimming (5% body weight-bearing) for 8 weeks,60 minutes once day for 6 days once week.At 24 hours after the last swimming training,the cardiac function indexes including left ventricular end systolic internal meridian (LVDS),left ventricular end diastolic internal meridian (LVDD),left ventricular end systolic volume (LVVS),stroke volume (SV),Left ventricular ejection fraction (LVEF) and left ventricular shortening fraction (LVSF) were measured by color Doppler ultrasound under;After anesthetized with 10% chloral hydrate,the rats were performed with thoracotomy,and sinoatrial node was obtained,the morphological and structural changes of sinoatrial node were observed by hematoxylin-eosin staining,the distribution and positive expression of adipocytes in sinoatrial node were measured by oil red O staining,the expression of nerve marker protein gene product 9.5(PGP 9.5) in sinoatrial node was measured by immunohistochemistry.Results　The LVDS,LVDD and LVVS of rats in group B were significantly higher than those in group A,the SV,LVEF and LVSF of rats were significantly lower than those in group A(P＜0.05).There was no significant difference in LVDS,LVDD,LVVS,SV and LVEF of rats between the group C and group A (P>0.05),and LVSF was significantly higher than that in group A(P＜0.05).The LVDS,LVDD and LVVS of rats in the group C were significantly lower than those in group B,and SV,LVEF and LVSF were significantly higher than those in group B (P＜0.05).The SV of rats in the group D was significantly lower than that in group A (P＜0.05),LVSF was significantly higher than that in group A (P＜0.05),LVEF and LVSF were significantly higher than those in group B,and SV was significantly lower than that in group C (P＜0.05);there was no significant difference in other cardiac function indexes between group D and group A,group B and group C (P>0.05).Compared with group A,the sinoatrial node structure of rats in group B was defected,the cell space was widened and the muscle fibers were disordered;in group C,the morphology and structure of sinoatrial node of rats were good,the cell space was uniform and narrow,and the cytoplasmic staining was uniform;compared with group C,the morphology and structure of sinoatrial node of rats in group D were poor,and the cell gap was slightly widened.The positive expression of adipocytes in sinoatrial node area of rats in group B,group C and group D was significantly higher than that in group A (P＜0.05).The positive expression of adipocytes in sinoatrial node area of rats in group B was significantly higher than that in the group C and group D (P＜0.05).The positive expression of adipocytes in sinoatrial node area of rats in group D was significantly higher than that in the group C (P＜0.05).The positive expression of PGP 9.5 in sinoatrial node area of rats in group A was significantly higher than that in the group B and group D (P＜0.05).The positive expression of PGP 9.5 in sinoatrial node area of rats in the group C and group D was significantly higher than that in group B (P＜0.05).The positive expression of PGP 9.5 in sinoatrial node area of rats in the group C was significantly higher than that in the group D (P＜0.05).Conclusion　Long-term high-fat diet can lead to excessive fat infiltration and nerve atrophy in sinoatrial node area of rats,reduce the decline of cardiac function;long-term regular exercise can protect sinoatrial node and cardiac function in high-fat diet rats by inhibiting sinoatrial node fat infiltration and promoting nerve remodeling,and the effect of aerobic exercise is better than high-intensity exercise.

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更新日期/Last Update: 2021-10-05