[1]王利强,姚风芹,张俊河,等.早餐前后血脂对电化学发光免疫法检测血清甲胎蛋白效果的影响[J].新乡医学院学报,2020,37(11):010-10.[doi:10.7683/xxyxyxb.2020.11.007]
WANG Liqiang,YAO Fengqin,ZHANG Junhe,et al.Effect of blood lipids before and after breakfast on the results of serum alpha-fetoprotein detected by electrochemiluminescence immunoassay[J].Journal of Xinxiang Medical University,2020,37(11):010-10.[doi:10.7683/xxyxyxb.2020.11.007]
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早餐前后血脂对电化学发光免疫法检测血清甲胎蛋白效果的影响
《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]
- 卷:
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37
- 期数:
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2020年11
- 页码:
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010-10
- 栏目:
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临床研究
- 出版日期:
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2020-11-05
文章信息/Info
- Title:
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Effect of blood lipids before and after breakfast on the results of serum alpha-fetoprotein detected by electrochemiluminescence immunoassay
- 作者:
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王利强1; 姚风芹2; 张俊河3; 崔月龙4; 周绍娟5; 刘文君1
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（1.濮阳市安阳地区医院精准医学实验室，河南 安阳 455000；2.濮阳市安阳地区医院病案管理科，河南 安阳 455000；3.新乡医学院生物化学与分子生物学教研室，河南 新乡 453003；4.濮阳市安阳地区医院放疗科，河南
安阳 455000；5.濮阳市安阳地区医院健康体检中心，河南 安阳 455000）
- Author(s):
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WANG Liqiang1; YAO Fengqin2; ZHANG Junhe3; CUI Yuelong4; ZHOU Shaojuan5; LIU Wenjun1
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(1.Laboratory of Precision Medicine,Anyang District Hospital of Puyang City,Anyang 455000,Henan；2.Department of Medical Records Management，Anyang District Hospital of Puyang City,Anyang 455000,Henan;3.Department of Biochemistry and Molecular Biology,Xinxiang Medical College,Xinxiang 453003,Henan;4.Department of Radiotherapy,Anyang District Hospital of Puyang City,Anyang 455000,Henan;5.Physical Examination Center,Anyang Regional Hospital of Puyang City,Anyang 455000,Henan)
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- 关键词:
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脂类; 电化学发光免疫法; 甲胎蛋白
- Keywords:
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lipids; electrochemiluminescence immunoassay; alpha-fetoprotein
- 分类号:
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R446
- DOI:
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10.7683/xxyxyxb.2020.11.007
- 文献标志码:
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A
- 摘要:
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目的 探讨早餐前后血脂对电化学发光免疫法（ECLIA）检测肿瘤标志物甲胎蛋白（AFP）效果的影响。方法 选择濮阳市安阳地区医院2018年6月至2019年12月收治的原发性肝细胞癌（HCC）住院患者65例为HCC组，另选择同期收治的血清三酰甘油（TG）和胆固醇（CHOL）同时增高的门诊及住院高脂血症患者90例为高脂血症组，选择同期体检健康人员90例为正常对照组。所有受试者于同一日早餐前及早餐后2 h采集静脉血4 mL，应用全自动电化学发光免疫分析仪检测血清AFP水平，全自动生物化学分析仪检测CHOL和TG水平。分析各组受试者早餐前后AFP、CHOL、TG水平的差异，以及各组受试者早餐前后CHOL、TG水平差值与AFP水平差值的相关性。结果 高脂血症组和HCC组受试者早餐前、早餐后2 h血清AFP水平均显著高于正常对照组（P<0.05），HCC组受试者早餐前、早餐后2 h血清AFP水平均显著高于高脂血症组（P<0.05）。高脂血症组受试者早餐前和早餐后2 h 血清CHOL、TG水平均显著高于正常对照组和HCC组（P<0.05）；HCC组受试者早餐前和早餐后2 h 血清CHOL、TG水平与正常对照组比较差异无统计学意义（P>0.05）。正常对照组、高脂血症组和HCC组受试者早餐后2 h 血清AFP水平与早餐前比较差异均无统计学意义（P>0.05）；正常对照组、高脂血症组和HCC组受试者早餐后2 h 血清CHOL、TG水平均显著高于早餐前（P<0.05）。Pearson相关性分析结果显示，HCC组、高脂血症组及正常对照组受试者早餐前后CHOL、TG水平差值与AFP水平差值均无相关性（r=0.036、0.151，0.087、0.074，0.049，0.105；P>0.05）。结论 早餐前后血脂对ECLIA检测血清AFP水平无影响。
- Abstract:
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Objective To explore the effect of serum lipids before and after breakfast on the effect of electrochemiluminescence immunoassay (ECLIA) on detecting tumor marker alpha-fetoprotein (AFP).Methods A total of 65 patients with hepatocellular carcinoma(HCC) admitted to Anyang District Hospital of Puyang City from June 2018 to December 2019 were selected as HCC group.In addition,90 cases of outpatient and inpatient hyperlipidemia patients with increased serum triacylglycerol (TG) and cholesterol (CHOL) were selected as the hyperlipidemia group.At the same time,90 cases of healthy person during physical examination were selected as normal control group.Before breakfast and 2 h after breakfast,4 mL of venous blood was collected from everybody of all the subjects on the same day.The serum AFP level was detected by an automatic electrochemiluminescence immunoassay analyzer,the CHOL and TG levels were detected by an automatic biochemical analyzer.The difference levels of AFP,CHOL and TG of the subjects before breakfast and 2 h after breakfast in each group were analyzed,as well as the correlation between the difference values of CHOL,TG levels and the difference value of AFP before and after breakfast in each group were analyzed.Results Before breakfast and 2 h after breakfast,the serum AFP levels of the subjects in the hyperlipidemia group and HCC group were significantly higher than that in the normal control group (P<0.05).Before breakfast and 2 h after breakfast,the serum AFP level of the subjects in the HCC group was significantly higher than that in the hyperlipidemia group (P<0.05).Before breakfast and 2 h after breakfast,the serum CHOL and TG levels of the subjects in the hyperlipidemia group were significantly higher than those in the normal control group and HCC group (P<0.05);there was no statistically significant difference in the serum CHOL and TG levels of the subjects between the HCC group and normal control group (P>0.05).Two hours after breakfast,there was no statistically significant difference in the serum AFP levels of the subjects among the normal control group,hyperlipidemia group and HCC group (P>0.05);the serum CHOL and TG levels at 2 h after breakfast of the subjects in the normal control group,hyperlipidemia group and HCC group were significantly higher than those before breakfast (P<0.05). Pearson correlation analysis showed that there was no correlation between the difference levels of CHOL,TG before and after breakfast and the difference level of AFP of patients in the HCC group，the hyperlipidemia group and the normal control group (r=0.036,0.151,0.087,0.014,0.049,0.105;P>0.05).Conclusion Before and after breakfast,lipid factors have no effect on serum AFP levels detected by ECLIA.
参考文献/References:
［1］ 林丽.电化学发光法与酶联免疫吸附法在乙肝病毒感染中的诊断价值［J］.临床合理用药杂志，2019，12（12）：175-176.
［2］ QIN X Z，RUI J Z，XIA Y，et al.Multi-center performance evaluations of tacrolimus and cyclosporine electrochemiluminescence immunoassays in the Asia-Pacific region［J］.Ann Lab Med，2018，38（2）：85-94.
［3］ 黄象维，徐霞，陈小剑，等.不同程度的溶血、脂浊标本对电化学发光法检测HBsAg结果的影响［J］.中国卫生检验杂志，2015，25（17）：2917-2921.
［4］ 中华人民共和国国家卫生健康委员会医政医管局.原发性肝癌诊疗规范（2019年版）［J］.中华肝脏病杂志，2020，28（2）：112-128.
［5］ FERRARO S，PANZERI A，BRAGA F，et al.Serum α-fetoprotein in pediatric oncology：not a children′s tale［J］.Clin Chem Lab Med，2019，57（6）：783-797.
［6］ YUAN X，LONG W，LIU J，et al.Associations of serum markers screening for Down′s syndrome with pregnancy outcomes：a Chinese retrospective cohort study［J］.Clin Chim Acta，2019，489：130-135.
［7］ WANG X，CHEN Y，KUANG H，et al.Associations between maternal AFP and β-HCG and preterm birth［J］.Am J Perinatol，2019，36（14）：1459-1463.
［8］ 颜彦，林应标，夏川.标本溶血、脂血对实验室检验的影响及对比分析［J］.吉林医学，2020，41（4）：898-899.
［9］ 庄冶.血清标本发生溶血和脂血对生化检验结果的影响观察［J］.中国医药指南，2020，18（2）：112-113.
［10］ 汪媛，常中宝，储晓敏，等.电化学发光法、化学发光法与酶联免疫吸附试验检测梅毒抗体效果对比分析［J］.医学动物防制，2020，36（7）：712-715.
［11］ 李红.罗氏电化学发光检测法与化学发光法检测泌乳素的比较［J］.临床检验杂志：电子版，2018，7（1）：122-123.
［12］ 吴敏，陈明艳，刘爱胜.罗氏Cobas E602全自动电化学发光分析仪检测贫血指标的方法学性能验证［J］.国际检验医学杂志，2013，34（23）：3229-3231.
［13］ 万奖.探讨不同免疫检验方法检测乙肝病毒感染血清标志物的效果差异性［J］.中国医药指南，2020，18（3）：47-48.
［14］ KE H，ZHANG X，HUANG C，et al.Electrochemiluminescence evaluation for carbohydrate antigen 15-3 based on the dual-amplification of ferrocene derivative and Pt/BSA core/shell nanos-pheres［J］.Biosens Bioelectron，2018，103：62-68.
［15］ 尚红，王毓三，申子瑜.全国临床检验操作规程［M］.4版.北京：人民卫生出版社，2015：314-315.
更新日期/Last Update:
2022-03-15