[1]梁　辉,左中夫,牛雪红.过表达Cdh1蛋白对糖尿病大鼠视网膜神经元损伤的保护作用[J].新乡医学院学报,2020,37(7):617-621.[doi:10.7683/xxyxyxb.2020.07.004]
　LIANG Hui,ZUO Zhongfu,NIU Xuehong.Protective effect of the overexpression of Cdh1 protein on retinal neuron injury in diabetic rats[J].Journal of Xinxiang Medical University,2020,37(7):617-621.[doi:10.7683/xxyxyxb.2020.07.004]
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过表达Cdh1蛋白对糖尿病大鼠视网膜神经元损伤的保护作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
37
期数:
2020年7
页码:
617-621
栏目:
基础研究
出版日期:
2020-07-05

文章信息/Info

Title:
Protective effect of the overexpression of Cdh1 protein on retinal neuron injury in diabetic rats
作者:
梁　辉1; 左中夫2; 牛雪红1
(1.丹东市第一医院眼科，辽宁　丹东　118000；2.锦州医科大学解剖学教研室，辽宁　锦州　121001)
Author(s):
LIANG Hui1; ZUO Zhongfu2; NIU Xuehong1
(1.Department of Ophthalmology，the First Hospital of Dandong City，Dandong 118000，Liaoning Province，China;2.Department of Anatomy，Basic Medical College，Jinzhou Medical University，Jinzhou 121001,Liaoning Province，China)
关键词:
Cdh1蛋白; 糖尿病; Müller细胞; 胶质增殖
Keywords:
Cdh1 protein; diabetes mellitus; Müller cells; glial proliferation
分类号:
R774.1
DOI:
10.7683/xxyxyxb.2020.07.004
文献标志码:
A
摘要:
目的　探讨过表达Cdh1蛋白对糖尿病（DM）大鼠视网膜神经元损伤的影响及机制。方法　将40只雄性Sprague Dawley大鼠随机分为对照组、DM组、过表达Cdh1组及阴性病毒组，每组10只。DM组、过表达Cdh1组及阴性病毒组大鼠采用单次腹腔注射链脲佐菌素（STZ）55 mg·kg-1诱导DM模型。模型诱导成功后，过表达Cdh1组大鼠玻璃体内注射6 μL过表达Cdh1蛋白的慢病毒载体，阴性病毒组大鼠玻璃体内注射6 μL慢病毒载体稀释液，对照组和DM组大鼠不进行处理。12周后，免疫组织化学染色检测各组大鼠视网膜组织中Cdh1表达，免疫荧光法检测各组大鼠视网膜组织中胶质纤维酸性蛋白(GFAP)表达，Western blot法检测各组大鼠视网膜组织中GFAP、Cdh1蛋白相对表达量，苏木精-伊红染色法检测各组大鼠视网膜神经节细胞(RGC)数量。结果　DM组和阴性病毒组大鼠视网膜组织中Cdh1蛋白表达及RGC数量低于对照组和过表达Cdh1组，GFAP蛋白表达高于对照组和过表达Cdh1组(P<0.05)；对照组大鼠视网膜组织中Cdh1和GFAP蛋白表达均低于过表达Cdh1组，RGC数量高于过表达Cdh1组(P<0.05)；阴性病毒组大鼠视网膜组织中Cdh1、GFAP蛋白表达及RGC数量与DM组比较差异无统计学意义(P>0.05)。结论　慢病毒过表达 Cdh1 蛋白对DM大鼠视网膜Müller细胞胶质增殖具有一定的抑制作用，能够减轻RGC损伤。
Abstract:
Objective　To investigate the effect and mechanism of the overexpression of Cdh1 protein on retinal neuron injury in diabetes mellitus (DM) rats.Methods　Forty male Sprague Dawley rats were randomly divided into control group，DM group，Cdh1 overexpression group and negative virus group，with 10 rats in each group.The rats in the DM group，the Cdh1 overexpression group and the negative virus group were given single intraperitoneal injection of streptozotocin（55 mg·kg-1） to induce the DM model.After the models were successfully induced，the rats in the Cdh1 overexpression group were injected with 6 μL constructed lentiviral vector of overexpressing Cdh1 protein by intravitreal injection,the rats in the negative virus group was injected with 6 μL lentiviral dilution solution，while the rats in the control group and DM group were not treated.After 12 weeks，the expression of Cdh1 in retina tissues was detected by immunohistochemical staining，the expression of glial fibrillary acidic protein(GFAP) in retina tissues was detected by immunofluorescence，and the relative expression of GFAP and Cdh1 protein was detected by Western blot method，the number of retinal ganglion cell(RGC) was detected by hematoxylin-eosin staining.Results　The expression of Cdh1 protein in retina tissues and the number of RGC in the DM group and negative virus group were lower than those in the control group and the Cdh1 overexpression group，and the expression of GFAP protein in retina tissues was higher than that in the control group and the Cdh1 overexpression group (P<0.05);the expression of Cdh1 and GFAP protein in retina tissues in the control group was lower than that in the Cdh1 overexpression group，and the number of RGC was higher than that in the Cdh1 overexpression group(P<0.05).There was no significant difference in the expression of Cdh1 and GFAP protein in retina tissues and the number of RGC between the negative virus group and the DM group (P>0.05).Conclusion　Overexpression of Cdh1 protein by lentiviral vector can inhibit the glial proliferation of retinal Müller cells in DM rats，and it can reduce the damage of RGC.

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更新日期/Last Update: 2020-07-05