[1]张晓荣,戴红霞,魏红艳,等.呼吸窘迫综合征早产儿血清Clara细胞分泌蛋白水平变化及意义[J].新乡医学院学报,2020,37(4):347-350.[doi:10.7683/xxyxyxb.2020.04.010]
　ZHANG Xiaorong,DAI Hongxia,WEI Hongyan,et al.Changes and significance of serum Clara cell secretory protein in preterm infants with respiratory distress syndrome[J].Journal of Xinxiang Medical University,2020,37(4):347-350.[doi:10.7683/xxyxyxb.2020.04.010]
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呼吸窘迫综合征早产儿血清Clara细胞分泌蛋白水平变化及意义
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
37
期数:
2020年4
页码:
347-350
栏目:
临床研究
出版日期:
2020-04-05

文章信息/Info

Title:
Changes and significance of serum Clara cell secretory protein in preterm infants with respiratory distress syndrome
作者:
张晓荣1; 戴红霞2; 魏红艳1; 冯玉梅1; 冯小三1; 黄　琳1
（1.珠海市人民医院儿科，暨南大学附属珠海医院儿科，广东　珠海　519000；2.澳门理工学院健康科学及体育高等学校，澳门　999078）
Author(s):
ZHANG Xiaorong1; DAI Hongxia2; WEI Hongyan1; FENG Yumei1; FENG Xiaosan1; HUANG Lin1
(1.Department of Pediatrics,Zhuhai People′s Hospital,Zhuhai Hospital Affiliated to Jinan University,Zhuhai 519000,Guangdong Province,China;2.The School of Health Sciences and Sports,Macao Polytechnic Institute,Macao 999078,China)
关键词:
早产儿; 呼吸窘迫综合征; Clara细胞分泌蛋白
Keywords:
preterm infant; respiratory distress syndrome; Clara cell secretory protein
分类号:
R722.6
DOI:
10.7683/xxyxyxb.2020.04.010
文献标志码:
A
摘要:
目的　探讨呼吸窘迫综合征（RDS）早产儿血清Clara细胞分泌蛋白（CCSP）水平变化及临床意义。方法　选择2013年6月至2015年6月在珠海市人民医院出生的63例早产儿为研究对象，其中有RDS的30例早产儿纳入观察组，无RDS的33例早产儿纳入对照组，分别于出生2 h及第3、7、14天，采用双抗体夹心酶联免疫吸附法测定2组早产儿血清CCSP水平。结果　观察组早产儿出生2 h至第7天血清CCSP水平逐渐升高，第7天达峰值，第7～14天维持在相对稳定水平；对照组早产儿出生2 h至第3天血清CCSP水平逐渐升高，第3天达高峰，然后开始下降，第 7～14天维持在相对稳定水平。观察组早产儿出生2 h时血清CCSP水平显著低于出生第3、7、14天（t=-2.805、-3.898、-3.580，P＜0.05），观察组早产儿出生第3、7、14天时血清CCSP水平比较差异均无统计学意义（t=-1.092、-0.775、0.318，P＞0.05）。对照组早产儿出生第3天时血清CCSP水平显著高于出生2 h及第7、14天（t=3.834、2.627、2.818，P＜0.05），对照组早产儿出生2 h及第7、14天时血清CCSP水平比较差异均无统计学意义（t=-1.207、1.016、0.191， P＞0.05）。观察组早产儿出生2 h和第3天时血清CCSP水平显著低于对照组（t=2.878、2.658，P＜0.05），2组早产儿出生第7、14天时血清CCSP水平比较差异无统计学意义（t=0.776、1.146，P＞0.05）。结论　RDS早产儿血清CCSP水平降低，血清CCSP水平对预测早产儿RDS的发生、发展和转归有一定价值，其可作为早产儿发生RDS的生物标志物。
Abstract:
Objective　To investigate the changes and clinical significance of serum Clara cell secretory protein (CCSP) in premature infants with respiratory distress syndrome (RDS).Methods　A total of 63 preterm infants born in Zhuhai People′s Hospital from June 2013 to June 2015 were selected as the study objects,including 30 preterm infants with RDS in the observation group and 33 preterm infants without RDS in the control group.The serum CCSP level of preterm infants in the two groups was detected by double-antibody sandwich enzyme-linked immunosorbent assay at 2 hours and 3,7,14 days after birth.Results　In the observation group,the serum CCSP level of premature infants gradually increased from 2 hours to 7 days after birth,reached the peak value on the 7th day,and remained at a relatively stable level from the 7th to the 14th day.In the control group,the serum CCSP level of premature infants gradually increased from 2 hours to 3 days after birth,reached the peak value on the 3rd day,then began to decrease and maintained at a relatively stable level from the 7th to the 14th day.The serum CCSP level of premature infants at 2 hours after birth was significantly lower than that on the 3rd,7th and 14th day in the observation group (t=-2.805,-3.898,-3.580；P<0.05).There was no significant difference in serum CCSP level of premature infants in the observation group on the 3rd,7th and 14th day after birth (t=-1.092,-0.775,0.318；P>0.05).The serum CCSP level of premature infants on the 3rd day after birth was significantly higher than that at 2 hours and 7,14 days after birth in the control group (t=3.834,2.627,2.818；P<0.05).There was no significant difference in serum CCSP levels of premature infants at 2 hours and 7,14 days after birth in the control group (t=-1.207,1.016,0.191；P>0.05).The serum CCSP level of premature infants in the observation group was significantly lower than that in the control group at 2 hours and three days after birth (t=2.878,2.658；P<0.05).There was no significant difference in serum CCSP level of premature infants between the two groups on the 7th and 14th day after birth (t=0.776,1.146；P>0.05).Conclusion　The level of serum CCSP in RDS preterm infants is decreased.The level of serum CCSP has a certain value in predicting the occurrence,development and outcome of RDS in preterm infants,it can be used as a biomarker of RDS in preterm infants.

参考文献/References:

［1］　茹喜芳，冯琪.新生儿呼吸窘迫综合征的防治：欧洲共识指南2019年版［J］.中华新生儿科杂志，2019，34（3）：239-240.
［2］　中国医师协会新生儿科医师分会.早产儿呼吸窘迫综合征早期防治专家共识［J］.中华实用儿科临床杂志，2018，33（6）：438-440.
［3］　GUZM N-B RCENAS J,CALDER N-MOORE A,BAPTISTA-GONZ-LEZ H,et al.Clara cell protein expression in mechanically ventilated term and preterm infants with respiratory distress syndrome and at risk of bronchopulmonary dysplasia：a pilot study［J］.Can Respir J,2017,2017:8074678.
［4］　陈龙，史源.新生儿急性呼吸窘迫综合征：从概念到实践［J］.中华实用儿科临床杂志，2019，34（18）：1364-1367.
［5］　BARTH P J,WOLF M,RAMASWAMY A,et al.Distribution and number of Clara cells in the normal and disturbed development of the human fetal lung［J］.Pediatr Pathol,1994,14(4)：637-651.
［6］　BERNARD A,THIELEMANS N,LAUWERYS R,et al.Clara cell protein in human amniotic fluid：a potential marker of fetal lung growth［J］.Pediatr Res,1994,36(6):771-775.
［7］　DE LUCA D,MINUCCI A,COGO P,et al.Secretory phospholipase A2 pathway during pediatric acute respiratory distress syndrome：a preliminary study［J］.Pediatr Crit Care Med,2011,12(1)：e20-e24.
［8］　PANG M,WANG H,BAI J Z,et al.Recombinant rat CC16 protein inhibits LPS-induced MMP-9 expression via NF-kB pathway in rat tracheal epithelial cells［J］.Exp Biol Med (Maywood),2015,240(10):1266-1278.
［9］　PLOPPER C G,MANGO G W,HATCH G E,et al.Elevation of susceptibility to ozone-induced acute tracheobronchial injury in transgenic mice deficient in Clara cell secretory protein［J］.Toxicol Appl Pharmacol,2006,213(1)：74-85.
［10］　BERNARD A,THIELEMANS N,LAUWERYS R,et al.The renal handling of protein 1 (Clara cell protein)：effect of age,sex and renal dysfunction［J］.Contrib Nephrol,1993,101(10):66-70.
［11］　BRIANA D D,GOURGIOTIS D,BOUTSIKOU M,et al.Clara cell protein in full-term pregnancies：the in uence of intrauterine growth restriction［J］.Pediatr Pulmonol,2010,45(12):1186-1191 .
［12］　SARAFIDIS K,STATHOPOULOU T,DIAMANTI E,et al.Clara cell secretory protein (CC16) as a peripheral blood biomarker of lung injury in ventilated preterm neonates［J］.Eur J Pediatr,2008,167(11)：1297-1303.
［13］　林玲，彭好.肺泡表面活性物质在新生儿呼吸窘迫综合征中的微创应用［J］.中华实用儿科临床杂志，2019，34（23）：1834-1837.
［14］　康乐.不同通气方式对肺表面活性物质治疗的呼吸窘迫综合征早产儿脑氧代谢的影响［J］.新乡医学院学报，2018，35（12）：1115-1117，1121．
［15］　DE LUCA D,CAPOLUONGO E,RIGO V,et al.Secretory phospholipase A2 pathway in various types of lung injury in neonates and infants：a multicentre translational study［J］.BMC Pediatr,2011,11(1)：101-110.
［16］　SCHRAMA A J,BERNARD A,POORTHUIS B J,et al.Cord blood Clara cell protein CC16 predicts the development of bronchopulmonary dysplasia［J］.Eur J Pediatr,2008,167(11):1305-1312.
［17］　TRIGGIANI M,GRANATA F,FRATTINI A,et al.Activation of human inflammatory cells by secreted phospholipases A2［J］.Biochim Biophys Acta,2006,1761(11):1289-1300.
［18］　SINGH G,KATYAL S L.Clara cell proteins ［J］.Ann NY Acad Sci,2000,923:43-58.
［19］　SINGH G,KATYAL S L.Clara cells and Clara cell 10 kD proteins(CC10)［J］.Am J Respir Cell Mol Biol,1997,17(2):141-143.
［20］　ORWOLL B E,SAPRU A.Biomarkers in pediatric ARDS：future directions［J］.Front Pediatr,2016,4:55.
［21］　周丹，石芳，刘瀚旻.“新型”支气管肺发育不良生物标记物的研究现状［J］.中华妇幼临床医学杂志，2019，15（4）：357-362.

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更新日期/Last Update: 2020-04-05