[1]平贯芳,熊万成,邓智建.长链非编码RNA 浆细胞瘤转化迁移基因1在结直肠癌组织和细胞中的表达及临床意义[J].新乡医学院学报,2019,36(10):949-953.[doi:10.7683/xxyxyxb.2019.10.011]
　PING Guan-fang,XIONG Wan-cheng,DENG Zhi-jian.Expression and clinical significance of long-chain non-coding RNA plasmacytoma variant translocation gene 1 in colorectal cancer tissues and cells[J].Journal of Xinxiang Medical University,2019,36(10):949-953.[doi:10.7683/xxyxyxb.2019.10.011]
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长链非编码RNA 浆细胞瘤转化迁移基因1在结直肠癌组织和细胞中的表达及临床意义
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年10
页码:
949-953
栏目:
临床研究
出版日期:
2019-10-05

文章信息/Info

Title:
Expression and clinical significance of long-chain non-coding RNA plasmacytoma variant translocation gene 1 in colorectal cancer tissues and cells
作者:
平贯芳1; 熊万成2; 邓智建1
(1.新乡医学院第一附属医院药学部，河南　卫辉　453100；2.新乡医学院第一附属医院普外科，河南　卫辉　453100）
Author(s):
PING Guan-fang1; XIONG Wan-cheng2; DENG Zhi-jian1
（1.Department of Pharmacy,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China;2.Department of General Surgery,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China）
关键词:
长链非编码RNA; 浆细胞瘤转化迁移基因1; 结直肠癌; 小分子RNA干扰; 细胞增殖; 细胞凋亡
Keywords:
long-chain non-coding NRA; plasmacytoma variant translocation 1; colorectal cancer; small interfering RNA; cell proliferation; apoptosis
分类号:
R735.3+5
DOI:
10.7683/xxyxyxb.2019.10.011
文献标志码:
A
摘要:
目的　探讨长链非编码RNA（LncRNA）浆细胞瘤转化迁移基因1（PVT1）在结直肠癌(CRC)组织和细胞中的表达及临床意义。方法　选取2006年4月至 2011年3月在新乡医学院第一附属医院接受手术切除治疗的CRC患者的癌组织和配对癌旁组织标本112例，采用实时荧光定量聚合酶链反应（PCR）检测CRC组织和癌旁组织中PVT1 mRNA的表达；并分析PVT1 mRNA表达与结直肠癌患者临床病理特征的关系。培养CRC细胞系LoVo和RKO细胞，待细胞融合度达70%以上时随机分为siPVT1组（转染 PVT1干扰序列）和siRNA-对照序列组（转染阴性对照序列），转染后继续培养48 h。采用实时荧光定量PCR法检测LoVo和RKO细胞中PVT1mRNA表达，流式细胞术检测LoVo和RKO细胞增殖和凋亡情况；Transwell分析法检测LoVo和RKO细胞的侵袭和迁移能力。结果　CRC组织和癌旁组织中PVT1 mRNA高表达率分别为 61.61% (69/112)、44.64% (50/112)，CRC组织中PVT1 mRNA高表达率显著高于癌旁组织(χ2=6.472，P<0.05)。CRC组织中PVT1表达与患者的年龄及性别无关（P>0.05），与肿瘤直径、TNM 分期、淋巴结转移及远处转移有关(P<0.05)。siPVT1组LoVo、RKO细胞中PVT1 mRNA相对表达量均显著低于 siRNA-对照序列组(P<0.05)。培养0、24 h时，siPVT1组与siRNA-对照序列组LoVo细胞增殖能力比较差异无统计学意义(P>0.05)；培养 48、72、96 h 时，siPVT1组LoVo细胞增殖能力显著低于siRNA-对照序列组(P<0.05)。培养0 h时，siPVT1 组与siRNA-对照序列组RKO细胞增殖能力比较差异无统计学意义(P>0.05);培养24、48、72、96 h 时，siPVT1组RKO细胞增殖能力显著低于siRNA-对照序列组(P<0.05)。siPVT1组LoVo和RKO细胞凋亡率均显著高于siRNA-对照序列组(P<0.05)。siPVT1组LoVo、RKO细胞迁移和侵袭数均显著低于siRNA-对照序列组(P<0.01)。结论　PVT1在CRC组织中呈高表达，且与肿瘤的恶性程度有关，沉默LoVo、RKO细胞中PVT1可有效抑制细胞增殖、迁移、侵袭，并诱导细胞凋亡。
Abstract:
Objective　To investigate the expression and clinical significance of long-chain non-coding RNA plasmacytoma variant translocation gene 1 (PVT1) in colorectal cancer(CRC) tissues and cells.Methods　A total of 112 CRC samples and adjacent tissues were collected in the First Affiliated Hospital of Xinxiang Medical University from April 2006 to March 2011．The expression of PVT1 mRNA in CRC tissue and adjacent tissues was measured by real-time fluorescence polymerase chain reaction (PCR) and the correlation between the expression of PVT1 and clinicopathological features of patients with CRC was analyzed.Human CRC cells of LoVo and RKO were cultured and randomly divided into siRNA control sequence group (transfected negative sequence lentivirus) and siPVT1 group (transfected with lentivirus PVT1 siRNA) when the cell fusion degree was over 70%.The LoVo and RKO cells were cultured for 48 hours after transfection.The expressions of PVT1 mRNA in LoVo and RKO cells were measured by real-time fluorescence PCR.The proliferation and apoptosis of LoVo and RKO cells were detected by flow cytometry assay;the invasion and migration ability of LoVo and RKO cells were detected by Transwell assay.Results　The high expression rate of PVT1 mRNA in CRC tissues and adjacent tissues was 61.61% (69/112) and 44.64% (50/112) respectively,the high expression rate of PVT1 mRNA in CRC tissues was significantly higher than that in the adjacent tissues (χ2=6.472，P<0.05).The expression of PVT1 mRNA in CRC tissues was related to tumor size,the TNM grading,lymph node metastasis and distant metastasis(P<0.05),but it was not related to age and gender of patients(P>0.05).The relative expression of PVT1 mRNA in LoVo and RKO cells in the siPVT1 group was significantly lower than that in the siRNA-control sequence group(P<0.05).There was no significant difference in the proliferation ability of LoVo cells between the siPVT1 group and the siRNA-control sequence group at 0,24 hours after cultured (P>0.05)．At 48,72,96 hours after cultured,the proliferation ability of LoVo cells in the siPVT1 group was lower than that in the siRNA-control sequence group(P<0.05).There was no significant difference in the proliferation ability of RKO cells between the siPVT1 group and the siRNA -control sequence group at 0 hour after cultured (P>0.05)．At 24,48,72,96 hours after cultured,the proliferation ability of RKO cells in the siPVT1 group was lower than that in the siRNA-control sequence (P<0.05).The apoptotic rates of LoVo and RKO cells in siPVT1 group were significantly higher than those in the siRNA-control sequence group (P<0.05).The number of migrating and invading cells of LoVo and RKO in siPVT1 group was significantly lower than that in the siRNA-control sequence group (P<0.01).Conclusion　PVT1 is highly expressed in the CRC tissue and is related to the malignant degree of tumors.Silencing PVT1 in LoVo and RKO cells can effectively inhibit cell proliferation,migration,invasion and induce cell apoptosis.

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更新日期/Last Update: 2019-10-05