[1]杨成轩,贾　良,孙艳霞,等.共刺激因子B7同源体4在肺腺癌组织中的表达及临床意义[J].新乡医学院学报,2019,36(9):833-836.[doi:10.7683/xxyxyxb.2019.09.007]
　YANG Cheng-xuan,JIA Liang,SUN Yan-xia,et al.Expression of costimulate molecules B7 homolog 4 in lung adenocarcinoma tissues and its clinical significance[J].Journal of Xinxiang Medical University,2019,36(9):833-836.[doi:10.7683/xxyxyxb.2019.09.007]
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共刺激因子B7同源体4在肺腺癌组织中的表达及临床意义
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年9
页码:
833-836
栏目:
临床研究
出版日期:
2019-09-05

文章信息/Info

Title:
Expression of costimulate molecules B7 homolog 4 in lung adenocarcinoma tissues and its clinical significance
作者:
杨成轩1; 贾　良1; 孙艳霞1; 秦　琛1; 原　翔2
（1.新乡市第一人民医院乳腺科，河南　新乡　453000；2.河南科技大学第一附属医院分子生物实验室 河南省肿瘤表观遗传重点实验室，河南　洛阳　471000）
Author(s):
YANG Cheng-xuan1; JIA Liang1; SUN Yan-xia1; QIN Chen1; YUAN Xiang2
（1.Department of Mammary Gland,the First People′s Hospital of Xinxiang City,Xinxiang 453000，Henan Province,China；2.Molecular Biology Laboratory,the First Affiliated Hospital of Henan University of Science and Technology,Henan Key Laboratory of Epigenetics of Tumors,Luoyang 471000,Henan Province,China）
关键词:
肺腺癌; 共刺激分子; B7同源体4; 预后
Keywords:
lung adenocarcinoma; costimulate molecules; B7 homolog 4; prognosis
分类号:
R734.2
DOI:
10.7683/xxyxyxb.2019.09.007
文献标志码:
A
摘要:
目的　分析共刺激因子B7同源体4 （B7H4）在肺腺癌组织中的表达及临床意义。方法　选择2012年1月至2012年12月河南科技大学第一附属医院手术切除的100例肺腺癌患者癌组织及配对的癌旁组织（距肿瘤组织边缘>5 cm）石蜡包埋标本为观察对象，采用免疫组织化学法检测肺腺癌组织及癌旁组织中B7H4蛋白表达，分析B7H4蛋白表达与肺腺癌患者临床病理特征的关系；采用COX回归分析各因素对肺腺癌患者预后的影响，采用Kaplan-Meier生存曲线及Log-rank检验分析B7H4蛋白表达与患者生存时间的关系。结果　肺腺癌组织及癌旁组织中均可见细胞膜及细胞质出现棕黄色颗粒，B7H4蛋白呈阳性表达。肺腺癌组织中B7H4蛋白表达免疫组织化学总评分及高、中、低分化癌组织B7H4表达免疫组织化学评分均显著高于癌旁组织（P<0.05）；随着癌组织分化程度的降低，B7H4蛋白表达免疫组织化学评分逐渐升高（P<0.05）。B7H4蛋白表达与肺腺癌患者分化程度、淋巴结转移、TNM分期和5 a生存情况有关（P<0.05），与患者的性别、年龄、吸烟及饮酒无关（P>0.05）。淋巴结转移、TNM分期及B7H4蛋白表达是影响肺腺癌患者预后的独立危险因素（P<0.05）。B7H4蛋白表达阳性和B7H4蛋白表达阴性患者5 a生存率分别为2.17%和27.78%，B7H4蛋白表达阳性患者5 a生存率低于B7H4蛋白表达阴性患者（χ2=31.020，P<0.05）。结论　B7H4蛋白高表达可能促进了肺腺癌的发生、发展，抑制B7H4蛋白表达可能是肺腺癌靶向治疗的潜在靶点之一。
Abstract:
Objective　To analyze the expression of costimulate molecules　B7 homolog 4(B7H4) in lung adenocarcinoma and its clinical significance.Methods　One hundred paraffin-embedded specimens of lung adenocarcinoma tissues and matched para-cancerous tissues (more than 5 cm from the edge of the tumor tissue) from patients with lung adenocarcinoma surgically resected in the First Affiliated Hospital of Henan University of Science and Technology from January 2012 to December 2012 were selected as objects.The expressions of B7H4 protein in lung adenocarcinoma tissues and matched para-cancerous tissues were detected by immunohistochemical method.The relationship between B7H4 protein expression and clinicopathological characteristics of lung adenocarcinoma patients was analyzed.The influence of various factors on the prognosis of lung adenocarcinoma was analyzed by COX regression.The relationship between B7H4 protein expression and survival time of patients was analyzed by Kaplan-Meier survival curve and Log-rank test.Results　Brown granules were found in the cell membrane and cytoplasm of lung adenocarcinoma and para-carcinoma tissue,and B7H4 protein expression was positive.The total immunohistochemical scores of B7H4 protein expression and the immunohistochemical scores of B7H4 protein expression in highly differentiated,moderately differentiated and poorly differentiated cancer tissues were significantly higher than those of para-cancer tissues (P<0.05).In cancer tissues,the immunohistochemical scores of B7H4 protein expression increased gradually with the decrease of differentiation degree (P<0.05).The expression of B7H4 protein was correlated with differentiation degree,lymph node metastasis,TNM stage and 5-year survival of lung adenocarcinoma patients (P<0.05),and was not correlated with sex,age,smoking and alcohol consumption of patients (P>0.05).Lymph node metastasis,TNM stage and B7H4 expression were independent risk factors for the prognosis of patients with lung adenocarcinoma (P<0.05).The 5-year survival rate of B7H4 positive group and B7H4 negative group was 2.17% and 27.78%,respectively.The 5-year survival rate of B7H4 positive group was lower than that of the B7H4 negative group (χ2=31.02,P<0.05).Conclusion　The high expression of B7H4 may promote the occurrence and development of lung adenocarcinoma,and inhibiting B7H4 may be one of the potential targets for targeted therapy of lung adenocarcinoma.

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更新日期/Last Update: 2019-09-05