[1]陈松建,王书伟,张 改,等.铜绿假单胞菌临床感染特征及其产超广谱β-内酰胺酶危险因素分析[J].新乡医学院学报,2019,36(8):755-758.[doi:10.7683/xxyxyxb.2019.08.013]
 CHEN Song-jian,WANG Shu-wei,ZHANG Gai,et al.Clinical infections characteristic of Pseudomonas aeruginosa and it′s risk factors for producing extended-spectrum β-lactamase[J].Journal of Xinxiang Medical University,2019,36(8):755-758.[doi:10.7683/xxyxyxb.2019.08.013]
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铜绿假单胞菌临床感染特征及其产超广谱β-内酰胺酶危险因素分析
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年8
页码:
755-758
栏目:
临床研究
出版日期:
2019-08-05

文章信息/Info

Title:
Clinical infections characteristic of Pseudomonas aeruginosa and it′s risk factors for producing extended-spectrum β-lactamase
作者:
陈松建王书伟张 改李振江靳 静李亚辉
(河南医学高等专科学校病原生物学与免疫学教研室,河南 郑州 451193)
Author(s):
CHEN Song-jianWANG Shu-weiZHANG GaiLI Zhen-jiangJIN JingLI Ya-hui
(Department of Pathogen Biology and Immunology,Henan Medical College,Zhengzhou 451193,Henan Province,China)
关键词:
铜绿假单胞菌超广谱β-内酰胺酶肠杆菌基因间重复共有序列
Keywords:
Pseudomonas aeruginosaextended-spectrum β-plactamaseenterobacterial repetitive intergenic consensus
分类号:
R446.5
DOI:
10.7683/xxyxyxb.2019.08.013
文献标志码:
A
摘要:
目的 探讨郑州市2家医院临床分离铜绿假单胞菌的感染特征及其与产生超广谱β-内酰胺酶(ESBLs)的关系。方法 选取2016年1月至2017年8月郑州市2家医院临床标本中分离出的51株非克隆铜绿假单胞菌,采用K-B纸片扩散法筛选出产ESBLs铜绿假单胞菌,采用肠杆菌基因间重复共有序列(ERIC)-聚合酶链反应(PCR)对51株铜绿假单胞菌株进行同源性分析,采用单因素和Logistic回归分析探讨铜绿假单胞菌产ESBLs的危险因素。结果 51株铜绿假单胞菌共检测出产ESBLs菌株22株,产ESBLs菌株阳性率为43.1%。51株铜绿假单胞菌ERIC-PCR同源性分析显示I型29株,Ⅱ型17株,Ⅲ型5株。单因素分析显示,内科来源的菌株中产ESBLs阳性菌株所占比例(59.2%)显著高于ICU来源的菌株(20.0%)(P<0.05),ERIC-PCR分型I型菌株产ESBLs阳性率(55.2%)显著高于Ⅱ型菌株(23.5%)及Ⅱ、Ⅲ型菌株之和(27.3%)(P<0.05)。Logistic多因素分析显示,ERIC-PCR分型I型、内科来源菌株为铜绿假单胞菌产ESBLs的危险因素(P<0.05)。结论 郑州地区铜绿假单胞菌感染以内科多见,性别分布上以男性患者为主,同源性分析以Ⅰ型和Ⅱ型为主,内科来源及ERIC-PCR分型Ⅰ型菌株是郑州地区医院内铜绿假单胞菌产生ESBLs的危险因素。
Abstract:
Objective To investigate the clinical infections characteristic of Pseudomonas aeruginosa and it′s risk factors for producing extended-spectrum β-lactamase(ESBLs).Methods A total of 51 non-cloned Pseudomonas aeruginosa strains of were isolated from clinical specimens of two hospitals in Zhengzhou from January 2016 to August 2017.The ESBLs-producing Pseudomonas aeruginosa were screened by K-B slip diffusion method.The homology of 51 strains of Pseudomonas aeruginosa was analyzed by enterobacterial repetitive intergenic consensus(ERIC)- polymerase chain reaction(PCR)method.The risk factors of Pseudomonas aeruginosa producing ESBLs were analyzed by single factor and logistic regression analysis.Results Twenty-two ESBLs-producing Pseudomonas aeruginosa were detected from 51 Pseudomonas aeruginosa strains,the positive rate of ESBLs-producing strains was 43.1%.The ERIC-PCR homology analysis showed that 29 strains of type Ⅰ,17 strains of type Ⅱ and 5 strains of type Ⅲ.Univariate analysis showed that the proportion of ESBLs-producing strains from internal medicine (59.2%) was significantly higher than that from ICU (20.0%) (P<0.05).The positive rate of ESBLs-producing strains of type Ⅰ (55.2%) was significantly higher than that of type Ⅱ (23.5%) and type Ⅱ+type Ⅲ (27.3%) (P<0.05).Logistic multivariate analysis showed that type Ⅰ and internal medicine strains were risk factors of Pseudomonas aeruginosa producing ESBLs(P<0.05).Conclusion The Pseudomonas aeruginosa was a major cause of infection in internal medicine in Zhengzhou.The Pseudomonas aeruginosa infection are more prevalent among the male patients.Homology analysis shows that type Ⅰ and type Ⅱ are the major type. ERIC-PCR typeⅠand internal medicine strains are the risk of Pseudomonas aeruginosa producing ESBLs in hospitals of Zhengzhou.

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更新日期/Last Update: 2019-08-05