[1]陈　俭,李传荣,王瑞敏,等.B族Ⅰ型清道夫受体过表达对血小板源性生长因子诱导的冠状动脉平滑肌细胞增殖和迁移的影响[J].新乡医学院学报,2019,36(5):421-426.[doi:10.7683/xxyxyxb.2019.05.005]
　CHEN Jian,LI Chuan-rong,WANG Rui-min,et al.Effect of scavenger receptor class B typeⅠ overexpression on proliferation and migration of coronary artery smooth muscle cells induced by platelet-derived growth factor[J].Journal of Xinxiang Medical University,2019,36(5):421-426.[doi:10.7683/xxyxyxb.2019.05.005]
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B族Ⅰ型清道夫受体过表达对血小板源性生长因子诱导的冠状动脉平滑肌细胞增殖和迁移的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年5
页码:
421-426
栏目:
基础研究
出版日期:
2019-05-05

文章信息/Info

Title:
Effect of scavenger receptor class B typeⅠ overexpression on proliferation and migration of coronary artery smooth muscle cells induced by platelet-derived growth factor
作者:
陈　俭; 李传荣; 王瑞敏; 毛幼林; 黄　琼; 马心超; 陈　愿; 董加建; 宋　爽; 崔留义; 赵子明
（郑州市第七人民医院心内科，河南　郑州　450006）
Author(s):
CHEN Jian; LI Chuan-rong; WANG Rui-min; MAO You-lin; HUANG Qiong; MA Xin-chao; CHEN Yuan; DONG Jia-jian; SONG Shuang; CUI Liu-yi; ZHAO Zi-ming
（Department of Cardiology，the Seventh People′s Hospital of Zhengzhou，Zhengzhou 450006，Henan Province，China）
关键词:
B族I型清道夫受体; 人冠状动脉平滑肌细胞; 血小板源性生长因子; 增殖; 迁移
Keywords:
scavenger receptor class B type Ⅰ; human coronary artery smooth muscle cell; platelet-derived growth factor; proliferation; migration
分类号:
R363.2
DOI:
10.7683/xxyxyxb.2019.05.005
文献标志码:
A
摘要:
目的　探讨B族Ⅰ型清道夫受体（SR-BⅠ）在人冠状动脉平滑肌细胞（hCASMC）增殖和迁移过程中的作用及机制。方法　常规培养hCASMC，根据处理方法将细胞分为空白对照组、5 μg·L-1血小板源性生长因子BB（PDGF-BB）组、10 μg·L-1 PDGF-BB组、20 μg·L-1 PDGF-BB组、20 μg·L-1 PDGF-BB+腺病毒绿色荧光蛋白（Ad-GFP） 组（PDGF-BB+Ad-GFP组）、20 μg·L-1 PDGF-BB+Ad-GFP-SR-BⅠ组（PDGF-BB+Ad-GFP-SR-BⅠ组）。采用噻唑兰法检测各组细胞增殖情况，Western blot法检测各组细胞中SR-BⅠ表达，Transwell法检测各组细胞迁移情况。结果　空白对照组及5、10、20 μg·L-1 PDGF-BB组细胞中SR-BⅠ相对表达量分别为1.00±0.05、0.76±0.08、0.52±0.06、0.30±0.05，5、10、20 μg·L-1 PDGF-BB组细胞SR-BⅠ相对表达量均显著低于空白对照组（P<0.05），10、20 μg·L-1 PDGF-BB组细胞SR-BⅠ相对表达量均显著低于5 μg·L-1 PDGF-BB组（P<0.05），20 μg·L-1 PDGF-BB组细胞SR-BⅠ相对表达量显著低于10 μg·L-1 PDGF-BB组（P<0.05）。培养24、48、72 h时，5、10、20 μg·L-1 PDGF-BB组及PDGF-BB+Ad-GFP组、PDGF-BB+Ad-GFP-SR-BⅠ组细胞增殖能力均显著高于空白对照组（P<0.05），10、20 μg·L-1 PDGF-BB组及PDGF-BB+Ad-GFP组、PDGF-BB+Ad-GFP-SR-BⅠ组细胞增殖能力显著高于5 μg·L-1 PDGF-BB组（P<0.05），20 μg·L-1 PDGF-BB组、PDGF-BB+Ad-GFP组、PDGF-BB+Ad-GFP-SR-BⅠ组细胞增殖能力显著高于10 μg·L-1 PDGF-BB组（P<0.05）；PDGF-BB+Ad-GFP-SR-BⅠ组细胞增殖能力低于20 μg·L-1 PDGF-BB组和PDGF-BB+Ad-GFP组（P<0.05）；20 μg·L-1 PDGF-BB组与PDGF-BB+Ad-GFP组细胞增殖能力比较差异无统计学意义（P>0.05）。空白对照组、20 μg·L-1 PDGF-BB组、PDGF-BB+Ad-GFP组及PDGF-BB+Ad-GFP-SR-BⅠ组细胞迁移数分别为24.2±3.6、76.6±4.2、75.2±4.8和60.6±3.6，各组细胞迁移数比较差异有统计学意义（F=40.695，P<0.01）；20 μg·L-1 PDGF-BB组、PDGF-BB+Ad-GFP组及PDGF-BB+Ad-GFP-SR-BⅠ组细胞迁移数显著高于空白对照组，PDGF-BB+Ad-GFP-SR-BⅠ组细胞迁移数显著低于20 μg·L-1 PDGF-BB组、PDGF-BB+Ad-GFP组（P<0.05）；20 μg·L-1 PDGF-BB组与PDGF-BB+Ad-GFP组细胞迁移数比较差异无统计学意义（P>0.05）。结论　PDGF-BB能够促进hCASMC增殖和迁移，并减少SR-BⅠ表达，过表达SR-BⅠ能够抑制PDGF-BB的作用，抑制hCASMC增殖和迁移。
Abstract:
Objective　To investigate the role and mechanism of scavenger receptor class B typeⅠ （SR-BⅠ） in the proliferation and migration of human coronary artery smooth muscle cells （hCASMC）.Methods　hCASMC were routinely cultured and divided into blank control group，5 μg·L-1 platelet-derived growth factor BB （PDGF-BB） group，10 μg·L-1 PDGF-BB group，20 μg·L-1 PDGF-BB group，20 μg·L-1 PDGF-BB+adenovirus green fluorescent protein （Ad-GFP） group （PDGF-BB+Ad-GFP group） and 20 μg·L-1 PDGF-BB+Ad-GFP-SR-BⅠ group according to the treatment method.The cell proliferation was detected by thiazolam method，SR-BⅠ expression was detected by Western blot and the cell migration was detected by Transwell method.Results　The relative expression of SR-BⅠ in the blank control group and 5,10,20 μg·L-1 PDGF-BB group was 1.00±0.05,0.76±0.08,0.52±0.06 and 0.30±0.05,respectively.There was significant difference in the relative expression of SR-BⅠ in the each group.The relative expression of SR-BⅠ in the 5,10,20 μg·L-1 PDGF-BB group was significantly lower than that in the blank control group (P<0.05).The relative expression of SR-BⅠ in the 10,20 μg·L-1 PDGF-BB group was significantly lower than that in the 5 μg·L-1 PDGF-BB group (P<0.05),and the relative expression of SR-BⅠ in the 20 μg·L-1 PDGF-BB group was significantly lower than that in the 10 μg·L-1 PDGF-BB group (P<0.05).After culturing for 24,48,72 h,the proliferative ability in the 5,10,20 μg·L-1 PDGF-BB group,PDGF-BB+Ad-GFP group and PDGF-BB+Ad-GFP-SR-BⅠ group was significantly higher than that in the blank control group (P<0.05);the proliferative ability in the 10,20 μg·L-1 PDGF-BB group was significantly higher than that in the 5 μg·L-1 PDGF-BB group (P<0.05);the proliferative ability in the 20 μg·L-1 PDGF-BB group was significantly higher than that in the 10 μg·L-1 PDGF-BB group (P<0.05);the proliferative ability in the PDGF-BB+Ad-GFP-SR-BⅠ group was lower than that in the 20 μg·L-1 PDGF-BB group and PDGF-BB+Ad-GFP group (P<0.01);but there was no significant difference between the 20 μg·L-1 PDGF-BB group and PDGF-BB+Ad-GFP group (P>0.05).The number of migrating cells in the blank control group,20 μg·L-1 PDGF-BB group,PDGF-BB+Ad-GFP group and PDGF-BB+Ad-GFP-SR-BⅠ group was 24.2±3.6,76.6±4.2,75.2±4.8 and 60.6±3.6;there was significant difference in the number of migrating cells among the groups（F=40.695，P<0.01）;the number of migrating cells in the 20 μg·L-1 PDGF-BB group,PDGF-BB+Ad-GFP group and PDGF-BB+Ad-GFP-SR-BⅠ group was significantly higher than that in the blank control group;and the number of migrating cells in the PDGF-BB+Ad-GFP-SR-BⅠ group was lower than that in the 20 μg·L-1 PDGF-BB group and PDGF-BB+Ad-GFP group (P<0.05);but there was no significant difference between the 20 μg·L-1 PDGF-BB group and PDGF-BB+Ad-GFP group (P>0.05).Conclusion　PDGF-BB can promote the proliferation and migration of hCASMC，and reduce the expression of SR-BⅠ.SR-BⅠ overexpression can inhibit the effect of PDGF-BB and the proliferation and migration of PDGF-BB and hCASMC cells.

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更新日期/Last Update: 2019-05-05