[1]金　雯,王　波,杨　梅,等.桩蛋白表达对裸鼠肝癌细胞增殖的影响[J].新乡医学院学报,2019,36(3):224-227.[doi:10.7683/xxyxyxb.2019.03.005]
　JIN Wen,WANG Bo,YANG Mei,et al.Effect of paxillin expression on proliferation of hepatocellular carcinoma cells in nude mice[J].Journal of Xinxiang Medical University,2019,36(3):224-227.[doi:10.7683/xxyxyxb.2019.03.005]
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桩蛋白表达对裸鼠肝癌细胞增殖的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年3
页码:
224-227
栏目:
基础研究
出版日期:
2019-03-05

文章信息/Info

Title:
Effect of paxillin expression on proliferation of hepatocellular carcinoma cells in nude mice
作者:
金　雯1; 王　波1; 杨　梅1; 曹立宇2
（1.铜陵职业技术学院医学系基础医学教研室，安徽　铜陵　244061；2.安徽医科大学第一附属医院病理科，安徽　合肥　230032）
Author(s):
JIN Wen1; WANG Bo1; YANG Mei1; CAO Li-yu2
（1.Department of Basic Medicine，College of Medical，Tongling Polytechnic College，Tongling 244000，Anhui Province，China；2.Department of Pathology，the First Affiliated Hospital of Anhui Medical University，Hefei 230022，Anhui Province，China）
关键词:
桩蛋白; 基因下调; 肝细胞癌; 黏着斑激酶
Keywords:
paxillin; gene reduction; hepatocellular carcinoma; focal adhesion kinase
分类号:
R735.7
DOI:
10.7683/xxyxyxb.2019.03.005
文献标志码:
A
摘要:
目的　探讨裸鼠体内桩蛋白（PXN）表达对肝癌细胞增殖的影响。方法　将24只裸鼠随机分为对照组、短发夹RNA（shRNA） PXN阴性对照组和shRNA PXN组，每组8只。3组裸鼠背部分别植入正常SMMC-7721细胞、转染shRNA PXN空质粒的SMMC-7721细胞和转染shRNA PXN的SMMC-7721细胞，观察3组裸鼠体内肿瘤的生长状况；于7周末采血并处死裸鼠，切取肿瘤组织称质量，并用甲醛固定、石蜡包埋。采用免疫组织化学法检测3组裸鼠肿瘤组织中PXN、黏着斑激酶（FAK）蛋白表达，反转录聚合酶链反应检测3组裸鼠肿瘤组织中PXN、FAK mRNA表达，酶联免疫吸附试验法检测3组裸鼠血清PXN、FAK水平。结果　shRNA PXN组裸鼠肿瘤质量低于对照组和shRNA PXN阴性对照组（P<0.05），对照组与shRNA PXN阴性对照组裸鼠肿瘤质量比较差异无统计学意义（P>0.05）。shRNA PXN组肿瘤组织中PXN和FAK蛋白表达显著低于对照组和shRNA PXN阴性对照组（P<0.05），对照组与shRNA PXN阴性对照组肿瘤组织中PXN和FAK蛋白表达比较差异均无统计学意义（P>0.05）。shRNA PXN组裸鼠肿瘤组织中PXN和FAK mRNA表达显著低于对照组和shRNA PXN阴性对照组（P<0.05），对照组与shRNA PXN阴性对照组裸鼠肿瘤组织中PXN和FAK mRNA表达比较差异均无统计学意义（P>0.05）。shRNA PXN组裸鼠血清PXN和FAK水平显著低于对照组和shRNA PXN阴性对照组（P<0.05），对照组与shRNA PXN阴性对照组裸鼠血清PXN和FAK水平比较差异均无统计学意义（P>0.05）。结论　下调PXN表达可以抑制裸鼠肝癌细胞的增长，FAK表达量随PXN的下调而减少，联合检测PXN和FAK的表达有望成为肝癌防控的新靶点。
Abstract:
Objective　To study the effect of paxillin （PXN） expression on the proliferation of hepatocellular carcinoma cells in nude mice.Methods　Nude mice were randomly divided into control group，short hairpi(shRNA) PXN negative control group and shRNA PXN group，with 8 nude mice in each group.The nude mice in the three groups were implanted with normal SMMC-7721 cells，shRNA PXN negative control empty plasmid transfected SMMC-7721 cells and shRNA PXN transfected SMMC-7721 cells，and the growth of tumors of nude mice in the three groups was observed.Blood samples were collected at the end of the 7th week and the nude mice were sacrificed；the tumor tissues were obtained and weighted，and fixed with formaldehyde and embedded in paraffin.The expressions of PXN and focal adhesion kinase(FAK) protein in the tumor tissues of nude mice in the three groups were detected by immunohistochemistry.The expressions of PXN and FAK mRNA in the tumor tissue of nude mice in the three groups were detected by reverse transcription polymerase chain reaction.The levels of serum PXN and FAK of nude mice in the three groups were detected by enzyme-linked immunosorbent assay.Results　The mass of tumor of nude mice in the shRNA PXN group was significantly lower than that in the control group and shRNA PXN negative control group （P<0.05）；there was no significant difference in the weight of tumor of nude mice between the control group and shRNA PXN negative control group （P>0.05）.The expressions of PXN and FAK protein in tumor tissues of nude mice in shRNA PXN group were significantly lower than those in the control group and shRNA PXN negative control group （P<0.05）；there was no significant difference in the expression of PXN and FAK protein between the control group and the shRNA PXN negative control group （P>0.05）.The expressions of PXN and FAK mRNA in tumor tissues of nude mice in the shRNA PXN group were significantly lower than those in the control group and shRNA PXN negative control group （P<0.05）.There was no significant difference in the expression of PXN and FAK mRNA between the control group and shRNA PXN negative control group （P>0.05）.The levels of serum PXN and FAK of nude mice in the shRNA PXN group were significantly lower than those in the control group and shRNA PXN negative control group （P<0.05）；there was no difference in the levels of serum PXN and FAK between the control group and shRNA PXN negative control group （P>0.05）.Conclusion　Down-regulation of PXN expression can inhibit the growth of hepatocarcinoma in nude mice.The expression of FAK decreased with the decrease of PXN.Combined detection of PXN and FAK expression is expected to become a new target for hepatocellular carcinoma prevention and control.

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更新日期/Last Update: 2019-03-05