[1]段长恩,秦卫玲,周朝元,等.风湿性心脏病患者二尖瓣瓣膜组织中辅助性T细胞17和辅助性T细胞22的表达及临床意义[J].新乡医学院学报,2018,35(9):792-795.[doi:10.7683/xxyxyxb.2018.09.010]
　DUAN Chang-en,QIN Wei-ling,ZHOU Chao-yuan,et al.Expression and clinical significance of helper T cell 17 and helper T cell 22 in the mitral valve of patients with rheumatic heart disease[J].Journal of Xinxiang Medical University,2018,35(9):792-795.[doi:10.7683/xxyxyxb.2018.09.010]
点击复制

风湿性心脏病患者二尖瓣瓣膜组织中辅助性T细胞17和辅助性T细胞22的表达及临床意义
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
35
期数:
2018年9
页码:
792-795
栏目:
临床研究
出版日期:
2018-09-05

文章信息/Info

Title:
Expression and clinical significance of helper T cell 17 and helper T cell 22 in the mitral valve of patients with rheumatic heart disease
作者:
段长恩1; 秦卫玲2; 周朝元1; 韩培立1; 杨秀滨3
（1.新乡医学院第一附属医院心血管外科，河南省神经修复重点实验室，河南　卫辉　453100；2.新乡医学院第三附属医院血液内科，河南　新乡　453003；3.中国医学科学院北京协和医学院阜外心血管病医院心血管外科，北京　100037）
Author(s):
DUAN Chang-en1; QIN Wei-ling2; ZHOU Chao-yuan1; HAN Pei-li1; YANG Xiu-bin3
（1.Department of Cardiovascular Surgery，the First Affiliated Hospital of Xinxiang Medical University；Henan Key Laboratory of Neurol Regeneration，Weihui 453100，Henan Province，China；2.Department of Hematopathy，the Third Affiliated Hospital of Xinxiang Medical University，Xinxiang 453100，Henan Province，China；3.Fuwai Hospital，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100037，China）
关键词:
风湿性心脏病; 二尖瓣膜; 辅助性T细胞17; 辅助性T细胞22; 白细胞介素-17; 白细胞介素-22
Keywords:
rheumatic heart disease; mitral valve; helper T 17; helper T 22; interleukin-17; interleukin-22
分类号:
R473.6
DOI:
10.7683/xxyxyxb.2018.09.010
文献标志码:
A
摘要:
目的　探讨风湿性心脏病（RHD）患者二尖瓣瓣膜中辅助性T细胞（Th）17及Th22细胞的表达及临床意义。方法　选择新乡医学院第一附属医院心血管外科2016年5月至2017年5月确诊的40例RHD患者为观察组，另选择30例非风湿性心脏瓣膜病变患者为对照组。获取2组患者的瓣膜组织，应用流式细胞仪检测瓣膜组织中Th17及Th22细胞在CD4+T细胞中的比例；采用实时荧光定量反转录聚合酶链反应（qRT-PCR）检测瓣膜组织中白细胞介素（IL）-17和IL-22 mRNA的相对表达量；采用酶联免疫吸附试验检测瓣膜细胞培养上清液中IL-17和IL-22的水平。结果　观察组患者二尖瓣瓣膜组织中Th17和Th22细胞占CD4+T细胞的比例分别为（3.95±0.81）%和（3.24±0.68）%，对照组患者二尖瓣瓣膜组织中Th17和Th22细胞占CD4+T细胞的比例分别为（1.34±0.25）%和（0.83±0.20）%，观察组患者二尖瓣瓣膜组织中Th17和Th22细胞占CD4+T细胞的比例显著高于对照组 （P<0.05）。观察组患者瓣膜组织中IL-17及IL-22 mRNA相对表达量分别为5.03±1.43和4.67±1.12，对照组患者瓣膜组织中IL-17及IL-22 mRNA相对表达量分别为2.38±1.03和1.98±1.34，观察组患者二尖瓣瓣膜组织中IL-17、IL-22 mRNA相对表达量显著高于对照组（P<0.05）。观察组患者细胞培养上清液中IL-17和IL-22水平分别为（25.39±3.86）、（20.10±4.15）g·L-1，对照组患者细胞培养上清液中IL-17和IL-22水平分别为（8.34±2.13）、（10.13±2.15）g·L-1；观察组患者细胞培养上清液中IL-17、IL-22水平显著高于对照组（P<0.05）。结论　RHD患者二尖瓣瓣膜中Th17及Th22细胞比例及相应细胞因子的表达均明显升高，其在该疾病的发生、发展中可能起一定的促进作用。
Abstract:
Objective　To investigate the expression and clinical significance of the helper T cell 17（Th17） and helper T cell 22（Th22） in the mitral valve of patients with rheumatic heart disease(RHD).Methods　Forty patients with RHD were selected as the observation group；and thirty patients with nonrheumatic heart disease were selected as the control group.The valve tissues of patients in the two groups were obtained，and the proportion of Th17 cells and Th22 cells in the CD4+T cells in the mitral valve of the two groups were detected by flow cytometry；the expression of interleukin（IL）-17 and IL-22 mRNA in the valve tissues was detected by quantitative real-time polymerase chain reaction （qRT-PCR） and the levels of IL-17 and IL-22 in the cultured supernatant of valvular cells were detected by enzyme-linked immunosorbent assay.Results　The proportion of Th17 cells and Th22 cells in the CD4+T cells in the mitral valve of patients in observation group was （3.95±0.81）% and（3.24±0.68）%，and in control group was （1.34±0.25）% and（0.83±0.20）%；the proportion of Th17 cells and Th22 cells in the CD4+T cells in the mitral valve of patients in observation group was significantly higher than that in the control group（P<0.05）.The relative expression of IL-17 and IL-22 mRNA in the valve tissues of patients in the observation group was （5.03±1.43） and （4.67±1.12），and in the control group was （2.38±1.03）and（1.98±1.34）；the relative expression of IL-17 and IL-22 mRNA in the valve tissues of patients in the observation group was significantly higher than that in the control group （P<0.05）.The levels of IL-17 and IL-22 in the cultured supernatant of valvular cells in observation group were （25.39±3.86），（20.10±4.15） g·L-1 and in the control group were （8.34±2.13），（10.13±2.15）g·L-1；the levels of IL-17 and IL-22 in the cultured supernatant of valvular cells in observation group were significantly higher than those in the control group（P<0.05）.Conclusion　The proportion of Th17 and Th22 cells and the expression of related cytokines in the mitral valve of RHD patients are obviously increased，which may play a certain role in the occurrence and development of the disease.

参考文献/References:

［1］　马微.风湿性心脏瓣膜钙化的二尖瓣组织中巨噬细胞和炎性因子表达分析［J］.北华大学学报（自然科学版），2017，18（4）：466-469.
［2］　CHICHELNITSKIY E，HIMMELSEHER B，BACHMANN M，et al. Hypothermia promotes interleukin-22 expression and fine-tunes its biological activity［J］.Front Immunol，2017，8（3）：742.
［3］　BETTELLI E，OUKKA M，KUCHROO V K.Th17 cells in the circle of immunity and autoimmunity［J］.Nat Immunol，2007，8（4）：345-350.
［4］　张伯尧，张米，张锡武，等.钙化性心脏瓣膜间质细胞培养方法的应用与改进［J］.中华实验外科学杂志，2013，30（5）：1005-1006.
［5］　BREUNIG M，KLEINERT S，LEHMANN S，et al. Simple screening tools predict death and cardiovascular events in patients with rheumatic disease［J］.Scand J Rheumatol，2017，16（8）：1-8.
［6］　GUPTA S，GUPTA N，ARAND P，et al. Computerized acoustic cardiography correlates with echocardiography and invasive haemodynamics after percutaneous transvenous mitral commissurotomy［J］.J Med Eng Technol，2011，35（1）：59-64.
［7］　胡丕，余舜，胡知朋.人工机械瓣膜置换术后患者的社区康复管理［J］.中国全科医学，2014，17（12）：1434-1436.
［8］　BATZLOFF M R，FANE A，GORTON D，et al. Preclinical immunogenicity and safety of a group A streptococcal M protein-based vaccine candidate［J］.Hum Vaccin Immunother，2017，19（5）：287-290.
［9］　ENGELMAN D，MATAIKA R L，KADO J H，et al. Adherence to secondary antibiotic prophylaxis for patients with rheumatic heart disease diagnosed through screening in Fiji［J］.Curr Neurovasc Res，2016，13（4）：303-308.
［10］　WANG B，DILEEPAN T，BRISCOE S，et al. Induction of TGF-β1 and TGF-β1-dependent predominant Th17 differentiation by group A streptococcal infection［J］.Proc Natl Acad Sci USA，2010，107（13）：5937-5942.
［11］　王超，黄红光，任赞.Th17细胞在心血管疾病发病机制中的研究进展［J］.中国心血管杂志，2016，21（1）：73-75.
［12］　文韵，桂春，黄伟强，等.风湿性心脏病二尖瓣组织Th17细胞轴相关细胞因子表达水平［J］.中国老年学杂志，2016，36（1）：61-63.
［13］　BAS H D，BASER K，YAVUZ E，et al. A shift in the balance of regulatory T and T helper 17 cells in rheumatic heart disease［J］.J Investig Med，2014，62（1）：78-83.
［14］　GUILHERME L，KALIL J.Rheumatic heart disease：molecules involved in valve tissue inflammation leading to the autoimmune process and anti-S.pyogenes vaccine［J］.Front Immunol，2013，30（4）：352.
［15］　杨新超，姜曼，胡钢.Th22细胞及其细胞因子IL-22在冠心病外周血的表达及与冠脉病变的相关性研究［J］.长江大学学报（自然科学版），2017，14（4）：11-14.
［16］　万军，石磊，吉庆伟，等.Th22型免疫反应在动脉粥样硬化疾病中的动态变化［J］.中国循环杂志，2016，31（5）：454-458.

相似文献/References:

[1]杨毅,寇桂枝,杨凌,等.风湿性心脏病急性左心衰竭出现酷似亚急性心肌梗塞心电图一例[J].新乡医学院学报,1991,8(03):244.
[2]袁宇.风湿性心脏病心房颤动患者血清肿瘤坏死因子的变化及其与心功能的关系[J].新乡医学院学报,2008,25(06):581.
[3]段长恩,秦卫玲,智明星,等.瑞舒伐他汀对风湿性心脏病伴心房颤动患者心房成纤维细胞体外增殖与凋亡的影响[J].新乡医学院学报,2020,37(6):517.[doi:10.7683/xxyxyxb.2020.06.003]
　DUAN Changen,QIN Weiling,ZHI Mingxing,et al.Effect of rosuvastatin on proliferation and apoptosis of atrial fibroblasts in patients with rheumatic heart disease and atrial fibrillation in vitro[J].Journal of Xinxiang Medical University,2020,37(9):517.[doi:10.7683/xxyxyxb.2020.06.003]
[4]段长恩,周朝元,刘晓晨,等.血清心型脂肪酸结合蛋白在风湿性心脏病患者心功能评估中的应用价值[J].新乡医学院学报,2018,35(2):125.[doi:10.7683/xxyxyxb.2018.02.011]
　DUAN Chang-en,ZHOU Chao-yuan,LIU Xiao-chen,et al.Value of serum heart fatty acid binding protein in the assessment of cardiac function in patients with rheumatic heart disease[J].Journal of Xinxiang Medical University,2018,35(9):125.[doi:10.7683/xxyxyxb.2018.02.011]

更新日期/Last Update: 2018-09-05