[1]杨琳红,王维峰,朱金玲,等.抑癌基因PTEN单核苷酸多态性与鼻咽癌的相关性[J].新乡医学院学报,2018,35(6):478-482.[doi:10.7683/xxyxyxb.2018.06.007]
　YANG Lin-hong,WANG Wei-feng,ZHU Jin-ling,et al.Correlation between single nucleotide polymorphisms of tumor suppressor gene PTEN and nasopharyngeal carcinoma[J].Journal of Xinxiang Medical University,2018,35(6):478-482.[doi:10.7683/xxyxyxb.2018.06.007]
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抑癌基因PTEN单核苷酸多态性与鼻咽癌的相关性
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
35
期数:
2018年6
页码:
478-482
栏目:
临床研究
出版日期:
2018-06-05

文章信息/Info

Title:
Correlation between single nucleotide polymorphisms of tumor suppressor gene PTEN and nasopharyngeal carcinoma
作者:
杨琳红1; 王维峰2; 朱金玲3; 张淑红3; 范宗宪1; 佟琳琰1; 孙海燕1; 韩　琳4
（1.佳木斯大学附属第一医院耳鼻咽喉科，黑龙江　佳木斯　154000；2.佳木斯大学附属第一医院神经外科，黑龙江　佳木斯　154000；3.佳木斯大学遗传学教研室，黑龙江　佳木斯　154000；4.中国人民解放军第二二四医院五官科，黑龙江　佳木斯　154000）
Author(s):
YANG Lin-hong1; WANG Wei-feng2; ZHU Jin-ling3; ZHANG Shu-hong3; FAN Zong-xian1; TONG Lin-yan1; SUN Hai-yan1; HAN Lin4
（1.Department of Otolaryngology，the First Affiliated Hospital of Jiamusi University，Jiamusi 154000，Heilongjiang Province，China；2.Department of Neurosurgery，the First Affiliated Hospital of Jiamusi University，Jiamusi 154000，Heilongjiang Province，China；3.Department of Genetics，Jiamusi University，Jiamusi 154000，Heilongjiang Province，China；4.Department of Five Senses，the 224th Hospital of Chinese People′s Liberation Army，Jiamusi 154000，Heilongjiang Province，China）
关键词:
鼻咽癌; PTEN基因; 单核苷酸多态性; 易感基因
Keywords:
nasopharyngeal carcinoma; PTEN gene; single nucleotide polymorphisms; predisposing genes
分类号:
R739.6
DOI:
10.7683/xxyxyxb.2018.06.007
文献标志码:
A
摘要:
目的　探讨抑癌基因PTEN单核苷酸多态性与佳木斯地区汉族人群鼻咽癌的关系。方法　选取2008年9月至2018年1月佳木斯大学第一附属医院收治的132例鼻咽癌患者（鼻咽癌组）和73 例体检健康者（对照组）的血样，提取全基因组DNA，采用限制性片段长度多态性分析技术检测rs532678和rs701848的多态性，分析抑癌基因PTEN单核苷酸多态性与鼻咽癌的关系。结果　2组受试者rs532678和rs701848位点的基因型及等位基因频率分布均符合Hardy-Weinberg 遗传平衡定律（P>0.05）。对照组受试者PTEN基因rs532678位点的CC、CT、TT基因型频率分别为0.630、0.342、0.027，C、T等位基因频率分别为0.801、0.198；鼻咽癌组患者PTEN基因rs532678位点的CC、CT、TT基因型频率分别为0.716、0.265、0.015，C、T等位基因频率分别为0.852、0.147；2组受试者PTEN基因rs532678位点的基因型和等位基因频率分布比较差异均无统计学意义（P>0.05）。对照组受试者PTEN基因rs701848位点的CC、CT、TT基因型频率分别为0.657、0.342、0.000，C、T等位基因频率分别为0.828、0.171；鼻咽癌组患者PTEN基因rs701848位点的CC、CT、TT基因型频率分别为0.424、0.500、0.075，C、T等位基因频率分别为0.674、0.325；鼻咽癌组rs701848位点CT、TT基因型频率和T等位基因频率显著高于对照组（P<0.05），CC基因型频率和C等位基因频率显著低于对照组（P<0.05）。PTEN基因rs701848位点CT+TT基因型个体罹患鼻咽癌的风险较高（P<0.05，OR=2.606，95%可信区间下限为1.439，上限为4.720）。携带CT+TT基因型个体的鼻咽癌发病风险是携带CC型个体的2.606倍。结论　抑癌基因PTEN rs532678位点多态性与鼻咽癌易感性无关；rs701848位点多态性与鼻咽癌易感性相关，携带CT+TT基因型的个体具有较高的鼻咽癌发病风险。
Abstract:
Objective　To investigate the relationship between single nucleotide polymorphisms of tumor suppressor gene PTEN and nasopharyngeal carcinoma in Jiamusi Han population.Methods　The blood samples of 132 patients with nasopharyngeal carcinoma（nasopharyngeal carcinoma group） and 73 healthy people（control group） were selected from September 2008 to January 2018 in the First Affiliated Hospital of Jiamusi University.The whole genome DNA was extracted，and the polymorphisms of rs532678 and rs701848 were detected by restriction fragment length polymorphism analysis.The relationship between the polymorphism of PTEN gene and nasopharyngeal carcinoma was analyzed.Results　The genotype and allele frequency distributions of rs532678 and rs701848 loci were in line with the Hardy-Weinberg genetic balance law in the two groups（P>0.05）.The genotypic frequency of CC，CT and TT at the rs532678 locus of PTEN gene in the control group was 0.630，0.342 and 0.027 respectively；and the allele frequency of C and T was 0.801 and 0.198 respectively.The genotypic frequency of CC，CT and TT at the rs532678 locus of PTEN gene in the nasopharyngeal carcinoma group was 0.716，0.265 and 0.015 respectively；and the allele frequency of C and T was 0.852 and 0.147 respectively.There was no significant difference in genotype distribution and allele frequency distribution at the rs532678 locus of PTEN gene between the two groups（P>0.05）.The genotypic frequency of CC，CT and TT at the rs701848 locus of the PTEN gene in the control group was 0.657，0.342 and 0.000 respectively；and the allele frequency of C and T was 0.828 and 0.171 respectively.The genotypic frequency of CC，CT and TT at the rs701848 locus of PTEN gene in the nasopharyngeal carcinoma group was 0.424，0.500 and 0.075 respectively；and the allele frequency of C and T was 0.674 and 0.325 respectively.The frequencies of CT，TT genotype and T allele of rs701848 locus in the nasopharyngeal carcinoma group were significantly higher than those in the control group（P<0.05）.The frequencies of CC genotype and C allele in the nasopharyngeal carcinoma group were significantly lower than those in the control group（P<0.05）.The individual with CT+TT genotype at the rs701848 locus of PTEN gene had higher risk for nasopharyngeal carcinoma（P<0.05，OR=2.606，95% confidence interval：1.439-4.720）.The risk for nasopharyngeal carcinoma in the individual with CT+TT genotype was 2.606 times as much as the individual carrying CC genotype.Conclusion　The rs532678 polymorphism of PTEN gene is not associated with the susceptibility to nasopharyngeal carcinoma.The polymorphism of rs701848 locus is associated with the susceptibility to nasopharyngeal carcinoma.The individual carrying CT+TT genotype has higher risk for nasopharyngeal carcinoma.

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更新日期/Last Update: 2018-06-05