[1]王娇娇,刘向玲,路承彪,等.5-羟色胺2C受体对形觉剥夺性成年弱视大鼠离体脑片初级视皮层长时程增强的影响[J].新乡医学院学报,2018,35(6):464-468.[doi:10.7683/xxyxyxb.2018.06.004]
 WANG Jiao-jiao,LIU Xiang-ling,LU Cheng-biao,et al.Effect of 5-hydroxytryptamine-2C subunit receptor on long-term potentiation in primary visual cortex of form deprivation adult amblyopia rats[J].Journal of Xinxiang Medical University,2018,35(6):464-468.[doi:10.7683/xxyxyxb.2018.06.004]
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5-羟色胺2C受体对形觉剥夺性成年弱视大鼠离体脑片初级视皮层长时程增强的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
35
期数:
2018年6
页码:
464-468
栏目:
基础研究
出版日期:
2018-06-05

文章信息/Info

Title:
Effect of 5-hydroxytryptamine-2C subunit receptor on long-term potentiation in primary visual cortex of form deprivation adult amblyopia rats
作者:
王娇娇1刘向玲1路承彪2宋子宣1张 锐3张 地1
(1.新乡医学院第三附属医院眼科,河南 新乡 453003;2.新乡医学院生理与神经生物学教研室,河南 新乡 453003;3.运城市眼科医院眼科,山西 运城 044000)
Author(s):
WANG Jiao-jiao1LIU Xiang-ling1LU Cheng-biao2SONG Zi-xuan1ZHANG Rui3ZHANG Di1
(1.Department of Ophthalmology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,Henan Province,China;2.Laboratory of Physiology and Neurobiology,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;3.Department of Ophthalmology,Yuncheng Ophthalmology Hospital,Yuncheng 044000,Shanxi Province,China)
关键词:
5-羟色胺2C受体长时程增强视皮层弱视大鼠
Keywords:
5-hydroxytryptamine-2C subunit receptorlong-term potentiationvisual cortexamblyopiarats
分类号:
R33-3
DOI:
10.7683/xxyxyxb.2018.06.004
文献标志码:
A
摘要:
目的 探讨5-羟色胺2C受体(5-HT2CR)对形觉剥夺性成年弱视大鼠离体脑片视皮层V1M区长时程增强(LTP)的影响。方法 将16只2周龄Spargue Dawley大鼠随机分为正常对照组和单眼形觉剥夺组,每组8只。正常对照组大鼠不做任何处理;单眼形觉剥夺组大鼠行右侧眼睑缝合术制备单眼形觉剥夺性弱视模型,造模成功后饲养6周,然后处死2组大鼠,冠状切取400 μm厚视皮层脑组织切片并孵育于人工脑脊液中。根据人工脑脊液中加入的药物不同,将正常对照组大鼠视皮层切片作为A组,将剥夺眼对侧视皮层切片分为B、C、D和E组,将剥夺眼同侧视皮层切片分为F、G、H和I组。A、B、F组人工脑脊液中不加任何药物,C、G组加生理盐水,D、H组加10 μmol·L-15-羟色胺盐酸盐,E、I组加10 μmol·L-1 SB 242084和10 μmol·L-1 5-羟色胺盐酸盐。采用细胞外微电极记录法对各组大鼠视皮层组织切片进行电生理学实验,记录离体组织切片视皮层V1M区LTP并计算神经元场突触后电位(fPSP)斜率。结果 A、B、C、D、E、F、G、H、I组大鼠视皮层fPSP斜率分别为(198.1±13.5)%、(106.3±8.3)%、(106.3±8.3)%、(157.1±9.7)%、(102.6±4.7)%、(144.5±2.9)%、(144.5±2.9)%、(192.2±8.6)%和(129.7±13.5)%。A、B、F组大鼠视皮层fPSP斜率两两比较差异均有统计学意义(P<0.001),其中A组大鼠视皮层fPSP斜率高于B、F组(P<0.001),B组大鼠视皮层fPSP斜率低于F组(P<0.001)。D组大鼠视皮层fPSP斜率高于C组(t=-10.833,P<0.001);H组大鼠视皮层fPSP斜率高于D、G组(t=-6.841、-10.616,P<0.001);E组大鼠视皮层fPSP斜率低于D、I组(t=11.872、-3.910,P<0.001,P<0.05);I组大鼠视皮层fPSP斜率低于H组(t=9.911,P<0.001)。结论 单眼形觉剥夺可造成双侧视皮层神经元功能减退,5-羟色胺盐酸盐可通过5-HT2CR起到一定逆转作用。
Abstract:
Objective To investigate the effects of 5-hydroxytryptamine-2C subunit receptor (5-HT2CR) on long-term potentiation (LTP) of V1M region visual cortex of form deprivation adult amblyopia rats.Methods Sixteen two weeks old Spargue Dawley rats were randomly divided into normal control group and monocular form deprivation group,with 8 rats in each group.The rats in the normal control group were not given any intervention;the rats in the monocular form deprivation group were sutured the right eye lid to establish the monocular form deprivation amblyopia model.All rats were fed for 6 weeks after establishing the model successfully,then the rats in the two groups were sacrificed and the coronal examination of 400 μm thick cortical brain slices were incubated in artificial cerebrospinal fluid artificial cerebrospinal fluid(ACSF).According to the difference of drugs in ACSF,the visual cortex slices of rats in normal control group were selected as group A;the contralateral visual cortex slices of the deprivation eye were divided into group B,group C,group D and group E;the ipsilateral visual cortex slices of the deprivation eye were divided into group F,group G,group H and group I.The ACSF of group A,B and F did not added any drugs;the ACSF of group C and group G were added with physiological saline;the ACSF of group D and group H were added with 10 μmol·L-1 5-hydroxytryptamine hydrochloride;the ACSF of group E and group I were added with 10 μmol·L-1 SB 242084 and 10 μmol·L-1 5-hydroxytryptamine hydrochloride.The electrophysiology experiment was performed in all of the visual cortex slices by extracellular microelectrode recording and the visual cortex fidd postsynaptic potential(fPSP) slope of V1M region of the visual cortex was recorded.Results The fPSP in group A,B,C,D,E,F,G,H,I was (198.1±13.5)%,(106.3±8.3)%,(106.3±8.3)%,(157.1±9.7)%,(102.6±4.7)%,(144.5±2.9)%,(144.5±2.9)%,(192.2±8.6)% and (129.7±13.5)%,respectively.There was statistic difference in fPSP slope of visual cortex among the group A,B,F(P<0.001);the fPSP slope of visual cortex of rats in group A was significantly higher than that in the group B and group F(P<0.001);the fPSP slope of visual cortex of rats in group B was significantly lower than that in the group F(P<0.001).The fPSP slope of visual cortex in group D was significantly higher than that in the group C(t=-10.833,P<0.001);the fPSP slope of visual cortex in group H was significantly higher than that in the group D and group G(t=-6.841,-10.616;P<0.001).The fPSP slope of visual cortex in group E was significantly lower than that in the group D and group I(t=11.872,-3.910;P<0.001,P<0.05);the fPSP slope of visual cortex in group I was significantly lower than that in the group H(t=9.911,P<0.001).Conclusion Monocular deprivation can lead to the dysfunction of bilateral visual cortex neurons and 5-hydroxytryptamine hydrochloride can reverse this phenomenon through 5-HT2CR.

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更新日期/Last Update: 2018-06-05