[1]何三军,魏少军.宫颈癌相关miR-17-5p靶位点不同基因型检测分析[J].新乡医学院学报,2017,34(9):851-853.[doi:10.7683/xxyxyxb.2017.09.019]
　HE San-jun,WEI Shao-jun.Detection and analysis of different genotypes of cervical cancer-associated miR-17-5p target loci[J].Journal of Xinxiang Medical University,2017,34(9):851-853.[doi:10.7683/xxyxyxb.2017.09.019]
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宫颈癌相关miR-17-5p靶位点不同基因型检测分析
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年9
页码:
851-853
栏目:
临床研究
出版日期:
2017-09-05

文章信息/Info

Title:
Detection and analysis of different genotypes of cervical cancer-associated miR-17-5p target loci
作者:
何三军; 魏少军
（汉中市中心医院检验科，陕西　汉中　723000）
Author(s):
HE San-jun; WEI Shao-jun
（Department of Clinical Laboratory，Hanzhong Central Hospital，Hanzhong 723000，Henan Province，China）
关键词:
宫颈癌; miR-17-5p; 单核苷酸多态性; 靶位点; 基因型
Keywords:
cervical cancer; miR-17-5p; single nucleotide polymorphism; target site; genotype
分类号:
R737.33
DOI:
10.7683/xxyxyxb.2017.09.019
文献标志码:
A
摘要:
目的　检测宫颈癌相关miR-17-5p 靶位点的不同基因型，分析靶位点多态性对宫颈癌患病风险的影响。方法　选择2014年6月至2016年6月汉中市中心医院收治的宫颈癌患者250例及同期体检健康女性250例，分别采集受试者血样，采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）方法测定miR-17-5p的3个靶位点rs3741216、rs217727和rs2839702的基因型，应用SPSS 21.0在线软件计算基因多态性与宫颈癌患病风险的相关性。结果　3个候选单核苷酸多态性位点均符合Hardy-Weinberg平衡定律。在等位基因模型下，H19基因上的rs217727位点会显著增加宫颈癌的患病风险［OR=1.55，95% CI(1.21,2.32)，P=0.001］。遗传模型分析显示，rs217727位点在最佳模型（显性模型）下，携带A/G和A/A基因型的个体宫颈癌患病风险显著增加，是携带G/G基因型的1.65倍［OR=1.65，95% CI(1.14,2.28)，P=0.006］。结论　miR-17-5p靶位点rs217727的多态性与宫颈癌患病风险具有相关性，携带A/G和A/A基因型的个体宫颈癌患病风险显著增加。
Abstract:
Objective　To detect the different genotypes of cervical cancer-associated miR-17-5p target loci，and to analyze the influence of target site polymorphism on the risk of cervical cancer.Methods　A total of 250 cervical cancer patients and 250 healthy females were selected in Hanzhong Central Hospital from June 2014 to June 2016.The blood samples were collected from the subjects.The genotypes of the three target loci rs3741216，rs217727 and rs2839702 in miR-17-5p were determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）.The associativity between genetic polymorphisms and the risk of cervical cancer was calculated by SPSS 21.0 online software.Results　The three candidate SNP loci fitted the Hardy-Weinberg equilibrium law.In the allele model，the rs217727 locus on the H19 gene significantly increased the risk of cervical cancer ［OR=1.55，95% CI（1.21，2.32），P=0.001］.Genetic model analysis showed that rs217727 locus in the best model （dominant model），the risk of cervical cancer in the individuals carrying A/G and A/A genotypes increased significantly，and the risk of cervical cancer in the individuals carrying A/G and A/A genotypes was 1.65 times higher than that in the individuals carrying G/G genotype ［OR=1.65，95% CI（1.14，2.28），P=0.006］.Conclusion　The polymorphism of miR-17-5p target site rs217727 is associated with the risk of cervical cancer，the risk of cervical cancer in the individuals carrying A/G and A/A genotypes is significantly increased.

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更新日期/Last Update: 2017-09-05