[1]史雨晨,柳景华.成纤维细胞生长因子受体1在大鼠各组织中的表达[J].新乡医学院学报,2016,33(11):945-949.[doi:10.7683/xxyxyxb.2016.11.003]
 SHI Yu-chen,LIU Jing-hua.Expression of fibroblast growth factor receptor1 in the tissues of rats[J].Journal of Xinxiang Medical University,2016,33(11):945-949.[doi:10.7683/xxyxyxb.2016.11.003]
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成纤维细胞生长因子受体1在大鼠各组织中的表达
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年11
页码:
945-949
栏目:
基础研究
出版日期:
2016-11-05

文章信息/Info

Title:
Expression of fibroblast growth factor receptor1 in the tissues of rats
作者:
史雨晨柳景华
(首都医科大学附属北京安贞医院心内科 北京市心肺血管疾病研究所,北京 100029)
Author(s):
SHI Yu-chenLIU Jing-hua
(Department of Cardiology,Beijing Anzhen Hospital Affiliated to Capital Medical University;Beijing Institute of Heart,Lung and Blood Vessel Diseases,Beijing 100029,China)
关键词:
成纤维细胞生长因子受体1成纤维细胞生长因子大鼠组织特异性
Keywords:
fibroblast growth factor receptor 1fibroblast growth factorratstissues specificity
分类号:
R39
DOI:
10.7683/xxyxyxb.2016.11.003
文献标志码:
A
摘要:
目的 研究成纤维细胞生长因子受体1(FGFR1)mRNA及蛋白在大鼠各组织中的表达。方法 采用实时定量荧光聚合酶链式反应测大鼠脂肪、肝脏、心脏、血管、肾脏、肌肉等组织中FGFR1 mRNA表达;采用酶联免疫吸附法及免疫组织化学法检测各组织中FGFR1蛋白的表达。结果 FGFR1 mRNA 和蛋白在大鼠脂肪、肝脏、心脏、血管、肾脏、肌肉中均有表达,主要分布于细胞膜。FGFR1 mRNA在大鼠脂肪、肝脏、心脏、血管、肾脏、肌肉中表达量分别为2.623 0±0.524 9、1.003 0±0.077 2、6.755 0±0.278 4、1.613 0±0.320 1、4.833 0±0.421 5、6.123 0±0.348 7,FGFR1蛋白在以上各组织中的表达量分别为(10.670 0±1.197 0)、(0.356 0±0.105 5)、(0.860 0±0.163 9)、(5.152 0±0.801 4)、(0.186 0±0.046 7)、(2.110 0±0.161 9) μg·g-1,FGFR1 mRNA在各组织中的表达从高至低依次为心脏、肌肉、肾脏、脂肪、血管和肝脏;FGFR1蛋白在各组织中的表达量从高至低依次为脂肪、血管、肌肉、心脏、肝脏、肾脏。FGFR1 mRNA和蛋白在各组织中的表达量比较差异均有统计学意义(P<0.05,P<0.01)。结论 FGFR1在大鼠各主要组织器官中均有表达,主要分布于细胞膜,且在各组织中的表达存在一定差异。
Abstract:
Objective To study the expression of fibroblast growth factor receptor1(FGFR1) mRNA and protein in the tissues of rats.Methods The expression of FGFR1 mRNA in fat,liver,heart,blood vessel,kedney and muscle tissues was measured by real time-polymerase chain reaction;and the expression of FGFR1 protein was determined by enzyme linked immunosorbent assay and immunohistochemistry.Results FGFR1 mRNA and protein expressed in fat,liver,heart,blood vessel,kedney and muscle tissues of the rats;and mainly distributed in the cell membrane.The expression of FGFR1 mRNA in fat,liver,heart,blood vessel,kedney and muscle tissues was 2.623 0±0.524 9,1.003 0±0.077 2,6.755 0±0.278 4,1.613 0±0.320 1,4.833 0±0.421 5,6.123 0±0.348 7 respectively;the expression of FGFR1 protein in above tissues was (10.670 0±1.197 0),(0.356 0±0.105 5),(0.860 0±0.163 9),(5.152 0±0.801 4),(0.186 0±0.046 7),(2.110 0±0.161 9) μg·g-1 respectively;the expression of FGFR1 mRNA in heart,muscle,kidney,fat,blood vessel and liver tissue was decreased in turn;the expression of FGFR1 protein in fat,blood vessel,muscle,heart,liver and kidney tissue was decreased in turn;there were statistic difference of the expression of FGFR1 mRNA and protein in above each tissue of rats(P<0.05,P<0.01).Conclusion FGFR1 mRNA and protein expresses in the major tissues of rats,and mainly distribute in the cell membrane and the expression in each tissue has significant difference.

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更新日期/Last Update: 2016-11-05