[1]程　莹,张利彬,丁延峰,等.P2X7受体阻断剂亮蓝G对氧化应激所致红细胞氧化损伤的保护机制[J].新乡医学院学报,2016,33(6):462-465.[doi:10.7683/xxyxyxb.2016.06.004]
　CHENG Ying,ZHANG Li-bin,DING Yan-feng,et al.Protection mechanism of P2X7 receptor antagonist brilliant blue G to oxidative damage of erythrocyte[J].Journal of Xinxiang Medical University,2016,33(6):462-465.[doi:10.7683/xxyxyxb.2016.06.004]
点击复制

P2X7受体阻断剂亮蓝G对氧化应激所致红细胞氧化损伤的保护机制
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年6
页码:
462-465
栏目:
基础研究
出版日期:
2016-06-05

文章信息/Info

Title:
Protection mechanism of P2X7 receptor antagonist brilliant blue G to oxidative damage of erythrocyte
作者:
程　莹1; 2; 张利彬1; 丁延峰1; 刘建庄2; 李东亮1
（1.新乡医学院基础医学院生理学与神经生物学教研室，河南　新乡　453003；2.新乡医学院第二附属医院心血管内科，河南　新乡　453002）
Author(s):
CHENG Ying1; 2; ZHANG Li-bin1; DING Yan-feng1; LIU Jian-zhuang2; LI Dong-liang1
（1.Department of Physiology and Neurocsience，Basic Medical College，Xinxiang Medical University，Xinxiang 453003，Henan Province，China；2.Department of Vasculocardiology,the Second Affiliated Hospital of Xinxiang Medical University，Xinxiang 453002，Henan Province，China）
关键词:
红细胞; 氧化损伤; P2X7受体
Keywords:
erythrocyte; oxidative damage; P2X7 receptor
分类号:
R363.1
DOI:
10.7683/xxyxyxb.2016.06.004
文献标志码:
A
摘要:
目的　探讨P2X7受体阻断剂亮蓝G（BBG）对偶氮二异丁脒盐酸盐（AAPH）所致红细胞氧化损伤的保护机制。方法　取雄性Wistar大鼠红细胞，配成体积分数6.25%的红细胞悬液，分为对照组、AAPH组、AAPH+BBG组，对照组加入林格液1，AAPH 组加入林格液1、AAPH液，AAPH+BBG组加入林格液1、AAPH液 及BBG液，分别将3组标本放入水浴恒温振荡器中孵育1.5 h，检测比较3组标本的红细胞溶血率、渗透脆性及细胞内Ca2+浓度变化。结果　与对照组比较，AAPH组红细胞溶血率、渗透脆性及红细胞内Ca2+浓度显著增高（P<0.01）；与AAPH组比较，AAPH+BBG组红细胞溶血率、渗透脆性及红细胞内Ca2+浓度显著降低（P<0.05）。结论　P2X7受体阻断剂BBG可抑制氧化应激所致红细胞Ca2+内流，降低红细胞的渗透脆性，从而抑制AAPH对红细胞造成的氧化损伤。
Abstract:
Objective　To explore the protection mechanism of P2X7 receptor antagonist brilliant blue G（BBG） to oxidative damage of erythrocyte induced by 2，2-Azobis（2-methylpropionamidine）dihydrochloride（AAPH）.Methods　Erythrocytes were taken from normal male Wistar rats，6.25% volume fraction of erythrocyte suspension was obtained，and it was divided into control group，AAPH group and AAPH+BBG group.Control group was treated with Ringer solution 1，AAPH group was treated with Ringer solution 1 and AAPH liquid，AAPH+BBG group was treated with Ringer solution 1，AAPH liquid and BBG liquid.Then the three groups of specimens were respectively placed in water bath shaker and incubated for 1.5 h，the changes of hemolysis rate，osmotic fragility and intracellular Ca2+ concentration of the three groups were detected and compared.Results　Compared with the control group，hemolysis rate，osmotic fragility and intracellular Ca2+ concentration were increased significantly in AAPH group（P<0.01）.Compared with AAPH group，hemolysis rate，osmotic fragility and intracellular Ca2+ concentration were reduced significantly in AAPH+BBG group（P<0.05）.Conclusion　P2X7 receptor antagonist BBG can inhibit the oxidative damage of erythrocyte caused by AAPH through inhibiting the influx of Ca2+ and reducing the osmotic fragility of erythrocyte.

参考文献/References:

［1］　ASHA DEVI S，SUBRAMANYAM M V V，VANI R，et al.Adaptations of the antioxidant system in erythrocytes of trained adult rats：impact of intermittent hypobaric-hypoxia at two altitudes［J］.Comp Biochem Physiol C Toxicol Pharmacol，2005，140（1）：59-67.
［2］　OKAMOTO K，MARUYAMA T，KAJI Y，et al.Verapamil prevents impairment in filterability of human erythrocytes exposed to oxidative stress［J］.Jpn J Physiol，2004，54（1）：39-46.
［3］　SHIVA SHANKAR REDDY C S，SUBRAMANYAM M V V，VANI R，et al.In vitro models of oxidative stress in rat erythrocytes：effect of antioxidant supplements［J］.Toxicol In Vitro，2007，21（8）：1355-1364.
［4］　HEJL J L，SKALS M，LEIPZIGER J，et al.P2X receptor stimulation amplifies complement-induced haemolysis［J］.Pflugers Arch，2013，465（4）：529-541.
［5］　KACZMAREK-HJEK K，LORINCZI ，HAUSMANN R，et al.Molecular and functional properties of P2X receptors：recent progress and persisting challenges［J］.Purinergic Signal，2012，8（3）：375-417.
［6］　FREIKMAN I，RINGEL I，FIBACH E.Oxidative stress-induced membrane shedding from RBCs is Ca flux-mediated and affects membrane lipid composition［J］.J Membr Biol，2011，240（2）：73-82.
［7］　SHAIK N，ZBIDAH M，LANG F.Inhibition of Ca2+ entry and suicidal erythrocyte death by naringin ［J］.Cell Physiol Biochem，2012，30（3）：678-686.
［8］　ABED M，ARTUNC F，ALZOUBI K，et al.Suicidal erythrocyte death in end-stage renal disease［J］.J Mol Med （Berl），2014，92（8）：871-879.
［9］　ANDREWS D A，YANG L，LOW P S.Phorbol ester stimulates a protein kinase C-mediated agatoxin-TK-sensitive calcium permeability pathway in human red blood cells［J］.Blood，2002，100（9）：3392-3399.
［10］　LANG F，ABED M，LANG E，et al.Oxidative stress and suicidal erythrocyte death ［J］.Antioxid Redox Signal，2014，21（1）：138-153.
［11］　BOUGUERRA G，ALJANADI O，BISSINGER R，et al.Embelin-Induced phosphatidylserine translocation in the erythrocyte cell membrane［J］.Cell Physiol Biochem，2015，37（4）：1629-1640.
［12］　汪海涛，林洁，朱宏丽.氧化应激与血液系统疾病［J］.解放军医学院学报，2014，35（5）：512-515.
［13］　SKALS M，JORGENSEN N R，LEIPZIGER J，et al.Alpha-hemolysin from Escherichia coli uses endogenous amplification through P2X receptor activation to induce hemolysis［J］.Proc Natl Acad Sci USA，2009，106（10）：4030-4035.
［14］　LARSEN C K，SKALS M，WANG T，et al.Python erythrocytes are resistant to α-hemolysin from Escherichia coli［J］.J Membr Biol，2011，244（3）：131-140.
［15］　SKALS M，LEIPZIGER J，PRAETORIUS H A.Haemolysis induced by α-toxin from Staphylococcus aureus requires P2X receptor activation［J］.Pflugers Arch，2011，462（5）：669-679.

相似文献/References:

[1]赵晶平,赵晶石,杨义明,等.红细胞C_3b受体及免疫复合物测定方法的探讨[J].新乡医学院学报,1995,12(02):144.
[2]张卫中,许明策,史志红,等.肝硬化患者红细胞内镁测定及其临床意义[J].新乡医学院学报,2001,18(01):025.
[3]卢　娜,李　晓,刘冬玲,等.线粒体DNA氧化损伤在动脉粥样硬化发生、发展中的作用研究进展[J].新乡医学院学报,2021,38(3):201.[doi:10.7683/xxyxyxb.2021.03.001]
[4]赵颖丹,张天嵩,马　骏,等.细颗粒物2.5在肾小管上皮细胞氧化损伤中的作用及其调控机制[J].新乡医学院学报,2020,37(12):1118.[doi:10.7683/xxyxyxb.2020.12.004]
　ZHAO Yingdan,ZHANG Tiansong,MA Jun,et al.Role of particulate matter 2.5 in oxidative damage of renal tubular epithelial cells and its regulatory mechanism[J].Journal of Xinxiang Medical University,2020,37(6):1118.[doi:10.7683/xxyxyxb.2020.12.004]
[5]耿卢婧,孙智欣,李俞辰,等.温度对过氧化氢抑制前成骨细胞MC3T3-E1细胞增殖和成骨分化的影响[J].新乡医学院学报,2024,(2):109.[doi:10.7683/xxyxyxb.2024.02.002]
　GENG Lujing,SUN Zhixin,LI Yuchen,et al.Effect of temperature on the inhibitory effect induced by hydrogen peroxide on cell proliferation and osteogenic differentiation in preosteoblast MC3T3-E1 cells[J].Journal of Xinxiang Medical University,2024,(6):109.[doi:10.7683/xxyxyxb.2024.02.002]

更新日期/Last Update: 2016-06-05