[1]谷　争,侯田田,周度爽,等.脑黑质单侧注射鱼藤酮制备帕金森小鼠模型方法探讨[J].新乡医学院学报,2016,33(2):108-112.[doi:10.7683/xxyxyxb.2016.02.007]
　GU Zheng,HOU Tian-tian,ZHOU Du-shuang,et al.Establishment of Parkinson′s disease model induced by rotenone in mice[J].Journal of Xinxiang Medical University,2016,33(2):108-112.[doi:10.7683/xxyxyxb.2016.02.007]
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脑黑质单侧注射鱼藤酮制备帕金森小鼠模型方法探讨
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年2
页码:
108-112
栏目:
基础研究
出版日期:
2016-02-05

文章信息/Info

Title:
Establishment of Parkinson′s disease model induced by rotenone in mice
作者:
谷　争; 侯田田; 周度爽; 董高攀; 赵二奇; 赵　营
（新乡医学院药学院，河南　新乡　453003）
Author(s):
GU Zheng; HOU Tian-tian; ZHOU Du-shuang; DONG Gao-pan; ZHAO Er-qi; ZHAO Ying
（Pharmacy College of Xinxiang Medical University，Xinxiang 453003，Henan Province，China）
关键词:
鱼藤酮; 脑黑质; 多巴胺神经元; 帕金森病
Keywords:
rotenone; substantia nigra; dopaminergic neuron; Parkinson′s disease
分类号:
R747
DOI:
10.7683/xxyxyxb.2016.02.007
文献标志码:
A
摘要:
目的　探讨应用鱼藤酮建立帕金森病小鼠模型的方法。方法　取30只清洁级国立卫生研究院（NIH）小鼠，随机分为正常组、溶媒组和实验组，每组10只。实验组采用立体定向微量注射法，将溶解于二甲基亚砜（DMSO）的鱼藤酮注射入小鼠脑右侧黑质致密区；溶媒组以同样的方法仅注射相同体积的DMSO，正常组不做任何处理。观察3组小鼠的行为变化，并采用免疫组织化学法分析黑质酪氨酸羟化酶（TH）水平。结果　建模前，3组小鼠体质量、爬杆实验得分、悬挂实验得分、水平和垂直自由活动得分比较差异均无统计学意义（P>0.05）。建模后，3组小鼠体质量均高于建模前（P<0.05）；实验组小鼠体质量明显低于对照组和溶媒组（P<0.05）；对照组和溶媒组小鼠体质量比较差异无统计学意义（P>0.05）。建模后，正常组和溶媒组小鼠爬杆实验得分、悬挂实验得分、水平和垂直自由活动得分与建模前比较差异均无统计学意义（P>0.05）；实验组小鼠爬杆实验得分、悬挂实验得分、水平和垂直自由活动得分较建模前、正常组和溶媒组建模后明显下降（P<0.05）。建模后，正常组和溶媒组小鼠两侧脑黑质中TH阳性神经元数量比较差异无统计学意义（P>0.05）；实验组小鼠右侧脑黑质中TH阳性神经元数量低于左侧（P<0.05）。3组小鼠左侧阳性神经元数量比较差异均无统计学意义（P>0.05）；实验组右侧阳性神经元数量低于正常组和溶媒组（P<0.05）。结论　鱼藤酮能选择性损毁黑质多巴胺能神经元，可较快建立稳定的、成功率较高的帕金森模型。
Abstract:
Objective　To explore the method of establishing Parkinson′s disease model which were induced by rotenone in mice.Methods　Thirty clean grade national institute of health（NIH） mice were randomly divided into experiment group，vehicle group and normal group，with 10 mice in each group.The rotenone was dissolved in dimethyl sulfoxide（DMSO） and then was injected into the right substantia nigra pars compacta in brain of mice by stereotactic microinjection in experiment group.Mice in vehicle group were injected with the same volume of DMSO into the right substantia nigra pars compacta by stereotactic microinjection.Mice in normal group were not given any treatment.Behavioral changes of mice were observed in the three groups.The immunohistochemical method was taken to analyze the level of tyrosine hydroxylase（TH） in substantia nigra.Results　There was no statistic difference of body weight，jungle pole scores，suspension test scores，horizontal swing and vertical swing test scores of mice before modeling among the normal group，vehicle group and experiment group（P>0.05）.After modeling，the body weight of mice in normal group，vehicle group and experiment group were higher than those before modeling（P<0.05）；the body weight of mice in experiment group was significantly lower than that in normal group and vehicle group（P<0.05）；there was no statistic difference of body weight of mice between the normal group and vehicle group（P>0.05）.There was no statistic difference of jungle pole scores，suspension test scores，horizontal swing and vertical swing test scores of mice before and after modeling in normal group and experiment group（P>0.05）.After modeling，the jungle pole scores，suspension test scores，horizontal swing and vertical swing test scores of mice in experiment group were significantly lower than those before modeling of experiment group and after modeling normal group and vehicle group（P<0.05）.After modeling，there was no statistic difference of the number of TH positive neuron between the left and right substantia nigra in normal group and vehicle group（P>0.05）；the number of TH positive neuron of right substantia nigra was significantly lower than that of left substantia nigra in experiment group（P<0.05）；there was no statistic difference of the number of TH positive neuron in left substantia nigra among normal group，vehicle group and experiment group（P>0.05）；the number of TH positive neuron of right substantia nigra in experiment group was significantly lower than that in normal group and vehicle group（P<0.05）.Conclusion　Rotenone can selectively damage dopaminergic neurons in the substantia nigra，and can quickly establish the Parkinson′s disease model.

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更新日期/Last Update: 2016-02-05