[1]霍书华,钟根深,许芝山,等.细胞因子诱导杀 伤细胞联合p38抑制剂对食管癌EC109细胞的作用[J].新乡医学院学报,2016,33(2):087-90.[doi:10.7683/xxyxyxb.2016.02.002]
　HUO Shu-hua,ZHONG Gen-shen,XU Zhi-shan,et al.Effect of cytokine induced killer cells combined with p38 inhibitors on esophageal carcinoma cells EC109[J].Journal of Xinxiang Medical University,2016,33(2):087-90.[doi:10.7683/xxyxyxb.2016.02.002]
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细胞因子诱导杀 伤细胞联合p38抑制剂对食管癌EC109细胞的作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年2
页码:
087-90
栏目:
基础研究
出版日期:
2016-02-05

文章信息/Info

Title:
Effect of cytokine induced killer cells combined with p38 inhibitors on esophageal carcinoma cells EC109
作者:
霍书华1; 钟根深2; 许芝山2; 李静雅2; 赵宝生1; 李汉臣1
（1.新乡医学院第一附属医院胸外科，河南　卫辉　453100；2.新乡医学院第一附属医院　河南省神经病学研究所，河南　卫辉　453100）
Author(s):
HUO Shu-hua1; ZHONG Gen-shen2; XU Zhi-shan2; LI Jing-ya2; ZHAO Bao-sheng1; LI Han-chen1
（1.Department of Thoracic Surgery，the First Affiliated Hospital of Xinxiang Medical University，Weihui 453100，Henan Province，China；2.The First Affiliated Hospital of Xinxiang Medical University，Henan Provincal Institute of Neurology，Weihui 453100，Henan Province，China）
关键词:
食管癌; 细胞因子诱导杀 伤细胞; p38抑制剂; 流式细胞术
Keywords:
esophageal carcinoma; p38 inhibitor; cytokine induced killer cell; flow cytometry
分类号:
R731
DOI:
10.7683/xxyxyxb.2016.02.002
文献标志码:
A
摘要:
目的　探讨细胞因子诱导杀 伤（CIK）细胞联合p38抑制剂对食管癌EC109细胞的作用。方法　常规分离健康人外周血单个核细胞，并诱导为CIK细胞，流式细胞仪检测其免疫表型；将食管癌EC109细胞分为空白对照组（不做处理）、p38抑制剂组（加入p38抑制剂）、CIK组（加入培养14 d的CIK细胞）和联合组（加入p38抑制剂联合培养14 d的CIK细胞）。乳酸脱氢酶释放法检测各组食管癌EC109细胞的存活率；流式细胞仪检测各组食管癌EC109细胞的周期阻滞率和凋亡率。结果　空白对照组、p38抑制剂组、CIK组及联合组EC109细胞存活率分别为100.00%、75.00%、50.00%和25.00%，对食管癌细胞G1期的阻滞率分别为31.46%、42.04%、50.16%和72.02%，食管癌EC109细胞凋亡率分别为7.15%、19.31%、42.15%和67.17%，其中p38抑制剂组、CIK组及联合组EC109细胞存活率、对G1期的阻滞率、细胞凋亡率均显著高于空白对照组（P<0.05），联合组以上指标均显著高于p38抑制剂组和CIK组（P<0.05）。结论　CIK联合p38抑制剂对食管癌EC109细胞具有较强的杀 伤作用。
Abstract:
Objective　To investigate the effect of cytokine induced killer（CIK）cells combined with p38 inhibitors on esophageal carcinoma cell EC109.Methods　Healthy human peripheral blood mononuclear cells were isolated and induced to CIK cells，and flow cytometry instrument was used to detect the immune phenotype.The esophageal carcinoma cell EC109 were divided into 4 groups：blank control group（without any treatment），p38 inhibitor group（adding p38 inhibitor），CIK group（adding CIK cells cultivated for 14 days）and combined group（adding p38 inhibitor and CIK cells).Lactate dehydrogenase release method was used to detect the survival rate of esophageal carcinoma cells EC109 in each group.The phase arresting rate and apoptosis rate of esophageal carcinoma cells EC109 in each group was detected by flow cytometry.Results　In blank control group，p38 inhibitor group，CIK group and combined group，the survival rate of esophageal carcinoma cells EC109 was 100.00%，75.00%，50.00% and 25.00% respectively，the G1 phase arresting rate of esophageal carcinoma cells EC109 was 31.46%，42.04%，50.16% and 72.02% and the apoptosis rate of them was 7.15%，19.31%，42.15% and 67.17% respectively.The survival rate，phase arresting rate and apoptosis rate of esophageal carcinoma cells EC109 in p38 inhibitor group，CIK group and combined group were significantly higher than those in blank control group（P<0.05），while they were significantly higher in combined group compared with those in p38 inhibitor group and CIK group（P<0.05）.Conclusion　Combination of CIK with p38 inhibitors has powerful effect of killing activity on esophageal cancer EC109 cells.

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更新日期/Last Update: 2016-02-05