[1]裴利宏,王国戗.多发性硬化症患者外周血辅助性T细胞17、调节性T细胞及其相关炎症因子和血清铁蛋白水平变化[J].新乡医学院学报,2023,40(1):055-58.[doi:10.7683/xxyxyxb.2023.01.010]
　PEI Lihong,WANG Guoqiang.Changes of peripheral blood helper T cell 17,regulatory T cell and their related inflammatory factors and serum ferritin levels in patients with multiple sclerosis[J].Journal of Xinxiang Medical University,2023,40(1):055-58.[doi:10.7683/xxyxyxb.2023.01.010]
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多发性硬化症患者外周血辅助性T细胞17、调节性T细胞及其相关炎症因子和血清铁蛋白水平变化
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
40卷
期数:
2023年1期
页码:
055-58
栏目:
临床研究
出版日期:
2023-01-05

文章信息/Info

Title:
Changes of peripheral blood helper T cell 17,regulatory T cell and their related inflammatory factors and serum ferritin levels in patients with multiple sclerosis
作者:
裴利宏; 王国戗
（新乡医学院第一附属医院检验科，河南　卫辉　453100）
Author(s):
PEI Lihong; WANG Guoqiang
(Department of Clinical Laboratory,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China)
关键词:
多发性硬化症; 铁蛋白; 辅助性T细胞17; 调节性T细胞; 肿瘤坏死因子-α; 白细胞介素-6
Keywords:
multiple sclerosis; ferritin; helper T cell 17; regulatory T cells; tumor necrosis factor-α; interleukin-6
分类号:
R744.51
DOI:
10.7683/xxyxyxb.2023.01.010
文献标志码:
A
摘要:
目的　观察多发性硬化症（MS）患者外周血辅助性T细胞17（Th17细胞）、调节性T细胞（Tr细胞）及血清铁蛋白、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）水平的变化，并探讨其在MS发生发展中的临床意义。方法　选择2018年1月至2021年12月新乡医学院第一附属医院收治的46例MS患者为MS组，另选择年龄、性别及体质量指数相匹配的46名健康者为对照组。采用流式细胞术检测2组受试者外周血Th17细胞和Tr细胞水平，并计算Th17细胞与Tr细胞的比值（Th17/Tr）；磁微粒化学发光法检测血清TNF-α、IL-6水平，免疫比浊法检测血清铁蛋白水平。结果　MS组患者外周血Th17细胞水平及Th17/Tr显著高于对照组，Tr细胞水平显著低于对照组（P<0.05）；复发进展期MS患者外周血Th17细胞水平及Th17/Tr显著高于缓解期患者，Tr细胞水平显著低于缓解期患者（P<0.05）；缓解期MS患者外周血Th17细胞、Tr细胞水平及Th17/Tr与对照组受试者比较差异无统计学意义（P>0.05）。MS组患者血清TNF-α、IL-6水平显著高于对照组（P<0.05），2组受试者血清铁蛋白水平比较差异无统计学意义（P>0.05）；复发进展期MS患者血清TNF-α、IL-6水平显著高于缓解期患者（P<0.05），复发进展期与缓解期MS患者血清铁蛋白水平比较差异无统计学意义（P>0.05）；缓解期MS患者血清TNF-α、IL-6和铁蛋白水平与对照组受试者比较差异无统计学意义（P>0.05）。MS患者外周血Th17/Tr与血清TNF-α、IL-6水平均呈正相关（P<0.05），MS患者外周血Th17、Tr细胞水平与血清TNF-α、IL-6水平无显著相关性（P>0.05）。结论　MS患者体内Th17/Tr及相关炎症因子TNF-α、IL-6水平升高，这可能与MS的发生、发展有关。
Abstract:
Objective　To observe the changes of peripheral blood helper T cell 17 (Th17 cell),regulatory T cell (Tr cell) and serum ferritin,tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6) levels in patients with multiple sclerosis (MS),and to investigate their clinical significance in the development of MS.Methods　Forty-six MS patients admitted to the First Affiliated Hospital of Xinxiang Medical University from January 2018 to December 2021 were selected as the MS group,and 46 healthy persons matched in age,sex and body mass index were selected as the control group.The levels of Th17 cells and Tr cells in peripheral blood of subjects in the two groups were detected by flow cytometry,and the ratio of Th17 cells to Tr cells (Th17/Tr) was calculated.The levels of serum TNF-α and IL-6 were detected by magnetic particle chemiluminescence method,and the level of serum ferritin was detected by immunoturbidimetry.Results　The levels of peripheral blood Th17 cells and Th17/Tr in the MS group were significantly higher than those in the control group,while the level of Tr cells was significantly lower than that in the control group (P<0.05).Compared with MS patients in remission stage,the levels of Th17 cells and Th17/Tr in peripheral blood of MS patients in relapse progression stage were significantly increased,while the level of Tr cells was significantly decreased (P<0.05).There was no significant difference in the levels of peripheral blood Th17,Tr cells and Th17/Tr between the MS patients in remission stage and the subjects in the control group (P>0.05).The levels of serum TNF-α and IL-6 of patients in the MS group were significantly higher than those in the control group (P<0.05),while there was no significant difference in serum ferritin level between the two groups (P>0.05).The levels of serum TNF-α and IL-6 in MS patients in relapse progression stage were significantly higher than those in patients in remission stage (P<0.05).There was no significant difference in serum ferritin level between MS patients in relapse progression stage and remission stage (P>0.05).There was no significant difference in serum TNF-α,IL-6 and ferritin levels between the patients with MS in remission stage and the subjects in the control group (P>0.05).The Th17/Tr in peripheral blood was positively correlated with the levels of serum TNF-α and IL-6 in MS patients (P<0.05),while there was no significant correlation between the levels of Th17 cells and Tr cells in peripheral blood and the levels of serum TNF-α and IL-6 in MS patients (P>0.05).Conclusion　The Th17/Tr and the levels of related inflammatory factors TNF-α and IL-6 increased in MS patients,which may be related to the occurrence and development of MS.

参考文献/References:

［1］　OLSSON T,BARCELLOS L F,ALFREDSSON L.Interactions between genetic,lifestyle and environmental risk factors for multiple sclerosis［J］.Nat Rev Neurol,2017,13(1):25-36.
［2］　HEDSTRM A K,BOMFIM I L,BARCELLOS L,et al.Interaction between adolescent obesity and HLA risk genes in the etiology of multiple sclerosis［J］.Neurology,2014,82(10):865-872.
［3］　THOMPSON A J,BANWELL B L,BARKHOF F,et al.Diagnosis of multiple sclerosis:2017 revisions of the McDonald criteria［J］.Lancet Neurol,2018,17(2):162-173.
［4］　PARNELL G P,BOOTH D R.The multiple sclerosis (MS) genetic risk factors indicate both acquired and innate immune cell subsets contribute to MS pathogenesis and identify novel therapeutic opportunities［J］.Front Immunol,2017,8(2):420-425.
［5］　朱红敏，刘智胜.2017年多发性硬化McDonald诊断标准修订版解读［J］.中华实用儿科临床杂志，2018，33（12）：895-899.
ZHU H M,LIU Z S.An interpretation of diagnosis of multiple sclerosis (2007 revisions of the McDonald criteria)［J］.Chin J Appl Clin Pediatr,2018,33(12):895-899.
［6］　HEDSTRM A K,OLSSON T,ALFREDSSON L.High body mass index before age 20 is associated with increased risk for multiple sclerosis in both men and women［J］.Mult Scler,2012,18(9):1334-1336.
［7］　MUNGER K L,BENTZEN J,LAURSEN B,et al.Childhood body mass index and multiple sclerosis risk:a long-term cohort study［J］.Mult Scler,2013,19(10):1323-1329.
［8］　WESNES K,MYHR K M,RIISE T,et al.Physical activity is associated with a decreased multiple sclerosis risk:the EnvIMS study［J］.Mult Scler,2018,24(2):150-157.
［9］　ABDOLLAHPOUR I,NEDJAT S,MANSOURNIA M A,et al.Lifestyle factors and multiple sclerosis:a population-based incident case-control study［J］.Mult Scler Relat Disord,2018,22:128-133.
［10］　张晓春，王晓明.多发性硬化中脑铁沉积的病理、临床及MRI研究新进展［J］.中国医学影像技术，2010，26（11）：2205-2208.
ZHANG X C,WANG X M.Progress of pathological,clinical and MRI study on iron deposition in the brain of the multiple sclerosis［J］.Chin J Med Imaging Technol,2010,26(11):2205-2208.
［11］　朴松兰，杨小存，马长春.多发性硬化免疫相关病因和发病机制的研究进展［J］.中国老年学杂志，2020，40（13）：2900-2904.
PU S L,YANG X C,MA C C.Research progress on immune related etiology and pathogenesis of multiple sclerosis［J］.Chin J Gerontol,2020,40(13):2900-2904.
［12］　IFERGAN I,WOSIK K,CAYROL R,et al.Statins reduce human blood-brain barrier permeability and restrict leukocyte migration:relevance to multiple sclerosis［J］.Ann Neurol,2006,60(1):45-55.
［13］　徐艳芳，张金文.多发性硬化的干细胞治疗进展［J］.实用医药杂志，2020，37（6）：562-566.
XU Y F,ZHANG J W.Advances in multiple sclerosis stem cell therapy［J］.Pract J Med Pharm,2020,37(6):562-566.
［14］　MURARO P A,PASQUINI M,ATKINS H L,et al.Long-term outcomes after autologous hematopoietic stem cell transplantation for multiple sclerosis［J］.JAMA Neurol,2017,74(4):459-469.
［15］　李丹，王黙，纪元，等.Th17/Treg细胞及相关炎性因子在复发缓解型多发性硬化中的作用及机制［J］.中国神经免疫学和神经病学杂志，2019，26（2）：89-93，98.
LI D,WANG M,JI Y,et al.Role of Th17/Treg cells and related cytokines in patients with relapsing remitting multiple sclerosis［J］.Chin J Neuroimmunol Neurol,2019,26(2):89-93,98.

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更新日期/Last Update: 2023-01-05