[1]李燕乐,柏效治,谢小娟.丙泊酚对卵巢癌SKOV3细胞迁移和侵袭能力的影响[J].新乡医学院学报,2021,38(9):812-817.[doi:10.7683/xxyxyxb.2021.09.003]
 LI Yanle,BAI Xiaozhi,XIE Xiaojuan.Effect of propofol on migration and invasion ability of ovarian cancer SKOV3 cells[J].Journal of Xinxiang Medical University,2021,38(9):812-817.[doi:10.7683/xxyxyxb.2021.09.003]
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丙泊酚对卵巢癌SKOV3细胞迁移和侵袭能力的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
38
期数:
2021年9
页码:
812-817
栏目:
基础研究
出版日期:
2021-09-05

文章信息/Info

Title:
Effect of propofol on migration and invasion ability of ovarian cancer SKOV3 cells
作者:
李燕乐柏效治谢小娟
(河南科技大学临床医学院 河南科技大学第一附属医院麻醉科,河南 洛阳 471003)
Author(s):
LI YanleBAI XiaozhiXIE Xiaojuan
(Department of Anesthesia,College of Clinical Medicine of Henan University of Science and Technology,the First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,Henan Province,China)
关键词:
丙泊酚卵巢癌细胞外调节蛋白激酶基质金属蛋白酶
Keywords:
propofolovarian cancerextracellular regulatory protein kinasematrix metalloproteinase
分类号:
R737.31
DOI:
10.7683/xxyxyxb.2021.09.003
文献标志码:
A
摘要:
目的 研究丙泊酚在体外对卵巢癌SKOV3细胞迁移和侵袭能力的影响,并探讨其潜在的分子机制。方法 将处于对数生长期的SKOV3细胞分为空白对照组、溶剂对照组、25 μmol·L-1丙泊酚组、50 μmol·L-1丙泊酚组和100 μmol·L-1丙泊酚组。空白对照组细胞给予正常培养基培养,溶剂对照组细胞给予与100 μmol·L-1丙泊酚组中丙泊酚等量的脂肪乳处理,25 μmol·L-1丙泊酚组、50 μmol·L-1丙泊酚组、100 μmol·L-1丙泊酚组细胞分别给予含25、50、100 μmol·L-1丙泊酚的培养基培养。分别采用划痕实验和Transwell侵袭实验检测各组SKOV3细胞的迁移和侵袭能力,Western blot法检测各组SKOV3细胞中MMP-2、MMP-9、ERK1/2蛋白的相对表达量。使用ERK siRNA 转染SKOV3细胞,然后分为空白对照组、溶剂对照组、25 μmol·L-1丙泊酚组、50 μmol·L-1丙泊酚组和100 μmol·L-1丙泊酚组,采用Western blot法检测转染ERK siRNA的各组SKOV3细胞中ERK1/2、MMP-2、MMP-9蛋白的相对表达量。结果 空白对照组、溶剂对照组、25 μmol·L-1丙泊酚组细胞迁移率、细胞侵袭能力比较差异无统计学意义(P>0.05);50 μmol·L-1丙泊酚组、100 μmol·L-1丙泊酚组细胞迁移率、细胞侵袭能力显著高于空白对照组、溶剂对照组、25 μmol·L-1丙泊酚组(P<0.05);50 μmol·L-1丙泊酚组与100 μmol·L-1丙泊酚组细胞迁移率比较差异无统计学意义(P>0.05);100 μmol·L-1丙泊酚组细胞侵袭能力显著高于50 μmol·L-1丙泊酚组(P<0.05)。空白对照组、溶剂对照组、25 μmol·L-1丙泊酚组SKOV3细胞中ERK1/2、MMP-2、MMP-9蛋白相对表达量比较差异无统计学意义(P>0.05);50 μmol·L-1丙泊酚组和100 μmol·L-1丙泊酚组SKOV3细胞中ERK1/2、MMP-2、MMP-9蛋白相对表达量均显著高于空白对照组、溶剂对照组和25 μmol·L-1丙泊酚组(P<0.05); 100 μmol·L-1丙泊酚组SKOV3细胞中ERK1/2蛋白相对表达量显著高于 50 μmol·L-1丙泊酚组(P<0.05);100 μmol·L-1丙泊酚组与50 μmol·L-1丙泊酚组SKOV3细胞中MMP-2和MMP-9蛋白相对表达量比较差异无统计学意义(P>0.05)。5组转染ERK siRNA的SKOV3细胞中MMP-2、MMP-9蛋白的相对表达量比较差异无统计学意义(P>0.05)。结论 丙泊酚能增强卵巢癌SKOV3细胞的迁移和侵袭能力,其作用机制可能与丙泊酚通过上调卵巢癌SKOV3细胞表达ERK1/2,进而促进MMP-2及MMP-9蛋白的表达有关。
Abstract:
Objective To study the effect of propofol on migration and invasion ability of ovarian cancer SKOV3 cells in vitro,and explore its potential molecular mechanism.Methods SKOV3 cells in logarithmic growth phase were divided into blank control group,solvent control group,25 μmol·L-1 propofol group,50 μmol·L-1 propofol group and 100 μmol·L-1 propofol group.The cells in blank control group were cultured with normal medium,the cells in solvent control group were cultured with fat emulsion(the amount of fat emulsion was same with the propofol of 100 μmol·L-1 propofol group),the cells in the 25 μmol·L-1 propofol group,50 μmol·L-1 propofol group and 100 μmol·L-1 propofol group were cultured with medium containing 25,50,100 mol · L-1 propofol respectively.The migrations and invasion ability of SKOV3 cells were detected by scratch test and Transwell invasion test respectively,and the relative expressions of MMP-2,MMP-9 and ERK1/2 protein of SKOV3 cells in each group were detected by Western blot.The SKOV3 cells were transfected with ERK siRNA,and then were divided into blank control group,solvent control group,25 μmol·L-1 propofol group,50 μmol·L-1 propofol group and 100 μmol·L-1 propofol group;the relative expressions of ERK1/2,MMP-2 and MMP-9 protein in SKOV3 cells transfected with ERK siRNA in above five groups were detected by Western blot.Results There was no significant difference in cell migration rate and invasion ability of SKOV3 cells among the blank control group,solvent control group,25 μmol·L-1 propofol group (P>0.05).The cell migration rate and invasion ability of SKOV3 cells in 50 μmol·L-1 propofol group and 100 μmol·L-1 propofol group were significantly higher than those in the blank control group,solvent control group,25 μmol·L-1 propofol group(P<0.05)there was no significant difference in cell migration rate of SKOV3 cells between the 50 μmol·L-1 propofol group and 100 μmol·L-1 propofol group(P>0.05)the invasion ability of SKOV3 cells in the 100 μmol·L-1 propofol group was significantly higher than that in the 50 μmol·L-1 propofol group(P<0.05).There was no significant difference in the relative expression of ERK1/2,MMP-2 and MMP-9 protein in SKOV3 cells among the blank control group,solvent control group,25 μmol·L-1 propofol group (P>0.05).The relative expression of ERK1/2,MMP-2 and MMP-9 protein in SKOV3 cells in the 50 μmol·L-1 propofol group and 100 μmol·L-1 propofol group were significantly higher than those in the blank control group,solvent control group,25 μmol·L-1 propofol group(P<0.05)the relative expression of ERK1/2 protein in SKOV3 cells in the 100 μmol·L-1 propofol group was significantly higher than that in the 50 μmol·L-1 propofol group(P<0.05)there was no significant difference in the relative expression of MMP-2,MMP-9 protein in SKOV3 cells between the 50 μmol·L-1 propofol group and 100 μmol·L-1 propofol group(P>0.05).There was no significant difference in the relative expression of MMP-2,MMP-9 protein in SKOV3 cells transfected with ERK siRNA among the five groups(P>0.05).Conclusion Propofol can enhance the migration and invasion ability of ovarian cancer SKOV3 cells,and its mechanism may be related to the up-regulation of the expression of ERK1/2 protein,and then up-regulating the expression of the MMP-2 and MMP-9 protein in ovarian cancer SKOV3 cells.

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更新日期/Last Update: 2021-09-05