[1]赵艳利,康志强,毛　雨,等.微RNA-152靶向调控内质网脂质Raft关联蛋白1对甲状腺乳头状癌细胞增殖和迁移的影响[J].新乡医学院学报,2021,38(9):801-805.[doi:10.7683/xxyxyxb.2021.09.001]
　ZHAO Yanli,KANG Zhiqiang,MAO Yu,et al.Effect of microRNA-152 on proliferation and migration of papillary thyroid carcinoma cells by targeting endoplasmic reticulum lipid raft associated protein 1[J].Journal of Xinxiang Medical University,2021,38(9):801-805.[doi:10.7683/xxyxyxb.2021.09.001]
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微RNA-152靶向调控内质网脂质Raft关联蛋白1对甲状腺乳头状癌细胞增殖和迁移的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
38
期数:
2021年9
页码:
801-805
栏目:
基础研究
出版日期:
2021-09-05

文章信息/Info

Title:
Effect of microRNA-152 on proliferation and migration of papillary thyroid carcinoma cells by targeting endoplasmic reticulum lipid raft associated protein 1
作者:
赵艳利; 康志强; 毛　雨; 张晓珂; 孙　玲; 汪　湲
（郑州大学附属郑州中心医院内分泌科，河南　郑州　450000）
Author(s):
ZHAO Yanli; KANG Zhiqiang; MAO Yu; ZHANG Xiaoke; SUN Ling; WANG Yuan
(Department of Endocrinology,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou 450000,Henan Province,China)
关键词:
微RNA-152; 甲状腺乳头状癌; 内质网脂质Raft关联蛋白1; 细胞增殖; 细胞侵袭; 细胞迁移
Keywords:
microRNA-152papillary thyroid carcinomaendoplasmic reticulum lipid raft associated protein 1cell proliferationcell invasioncell migration
分类号:
R742.5
DOI:
10.7683/xxyxyxb.2021.09.001
文献标志码:
A
摘要:
目的　探讨微RNA-152（miR-152）对甲状腺乳头状癌（PTC）细胞增殖、侵袭和迁移的影响及其作用机制。方法　将对数生长期PTC TPC-1细胞随机分为空白对照组、miR-con组（转染miRNA-mimics阴性对照）和miR-152组（转染miR-152-mimics），另取对数生长期正常甲状腺细胞株Nthy-ori 3-1作为Nthy-ori 3-1组。采用实时荧光定量聚合酶链反应法检测各组细胞中miR-152、内质网脂质Raft关联蛋白1(ERLIN1) mRNA表达，四唑盐比色法及平板克隆形成实验检测各组TPC-1细胞增殖能力，流式细胞仪检测各组TPC-1细胞凋亡情况，Transwell实验检测各组TPC-1细胞迁移、侵袭能力，Western blot法检测各组TPC-1细胞中ERLIN1蛋白表达。结果　空白对照组与miR-con组TPC-1细胞中miR-152、ERLIN1 mRNA和蛋白相对表达量、TPC-1细胞增殖抑制率、细胞克隆数、细胞凋亡率、细胞迁移数及侵袭数比较差异均无统计学意义（P＞0.05）。空白对照组、miR-con组、miR-152组TPC-1细胞中miR-152相对表达量显著低于Nthy-ori 3-1组（P＜0.05）；miR-152组TPC-1细胞中miR-152相对表达量显著高于空白对照组和miR-con组（P＜0.05）。与空白对照组、miR-con组比较，miR-152组TPC-1细胞增殖抑制率、细胞凋亡率显著升高，细胞克隆数、细胞迁移数及侵袭数显著降低（P＜0.05）。miR-152组TPC-1细胞中ERLIN1 mRNA及蛋白相对表达量均显著低于空白对照组和miR-con组（P＜0.05）。结论　过表达miR-152可下调TPC-1细胞中ERLIN1的表达，抑制TPC-1细胞增殖、侵袭和迁移，miR-152可能通过靶向调控ERLIN1参与PTC进展和转移。
Abstract:
Objective　To investigate the effect of microRNA (miR)-152 on the proliferation,invasion and migration of papillary thyroid carcinoma (PTC) cells and its mechanism.Methods　PTC TPC-1 cells in logarithmic growth phase were randomly divided into blank control group,miR-con group (transfected with miRNA-mimics negative control) and miR-152 group (transfected with miR-152-mimics),and the normal thyroid cells Nthy-ori 3-1 were taken as the Nthy-ori 3-1 group.The expressions of miR-152 and endoplasmic reticulum lipid raft associated protein 1 (ERLIN1) mRNA in each group were detected by real-time fluorescence quantitative polymerase chain reaction,the proliferation ability of TPC-1 cells in each group was detected by methyl thiazolyl tetrazolium method and plate colony forming test,the apoptosis of TPC-1 cells in each group was detected by flow cytometry,the cell migration and invasion ability of TPC-1 cells in each group were detected by Transwell test,the expression of ERLIN1 protein of TPC-1 cells in each group was detected by Western blot.Results　There was no significant difference in the relative expression of miR-152 and ERLIN1 mRNA and protein,the inhibition rate of TPC-1 cells proliferation,the cell clone number,the rate of apoptosis,the number of cell migration and invasion between the blank control group and the miR-con group (P>0.05).The relative expression of miR-152 in the blank control group,miR-con group and miR-152 group was significantly lower than that in the Nthy-ori 3-1 group (P<0.05)the relative expression of miR-152 in the miR-152 group was significantly higher than that in the blank control group and the miR-con group (P<0.05).Compared with the blank control group and miR-con group,the inhibition rate of cell proliferation,apoptosis rate of TPC-1 cells in miR-152 group were significantly increased (P<0.05)and the cell clone number,migration and invasion ability of TPC-1 cells in miR-152 group were significantly decreased (P<0.05).The relative expressions of ERLIN1 mRNA and protein of TPC-1 cells in the miR-152 group were significantly lower than those in the blank control group and the miR-con group (P<0.05).Conclusion　Overexpression of miR-152 can down-regulate the expression of ERLIN1 in TPC-1 cells,inhibit the proliferation,invasion and migration of TPC-1 cells.It is suggested that miR-152 may be involved in the progression and metastasis of PTC by targeting ERLIN1.

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更新日期/Last Update: 2021-09-05