[1]张谷香,李书萍,李　波.虫草素对卵巢癌细胞中Fra-1和 p53表达的影响[J].新乡医学院学报,2021,38(8):714-718.[doi:10.7683/xxyxyxb.2021.08.003]
　ZHANG Guxiang,LI Shuping,LI Bo.Effect of cordycepin on the expression of Fra-1 and p53 in ovarian cancer cells[J].Journal of Xinxiang Medical University,2021,38(8):714-718.[doi:10.7683/xxyxyxb.2021.08.003]
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虫草素对卵巢癌细胞中Fra-1和 p53表达的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
38
期数:
2021年8
页码:
714-718
栏目:
基础研究
出版日期:
2021-08-05

文章信息/Info

Title:
Effect of cordycepin on the expression of Fra-1 and p53 in ovarian cancer cells
作者:
张谷香; 李书萍; 李　波
（长沙市第四医院妇产科，湖南　长沙　410006）
Author(s):
ZHANG Guxiang; LI Shuping; LI Bo
（Department of Obstetrics and Gynecology,the Fourth Hospital of Changsha,Changsha 410006,Hunan Province,China）
关键词:
卵巢癌SKOV3细胞; 虫草素; Fra-1; p53
Keywords:
ovarian cancer SKOV3 cellscordycepinFra-1p53
分类号:
R994.6
DOI:
10.7683/xxyxyxb.2021.08.003
文献标志码:
A
摘要:
目的　探讨虫草素对卵巢癌细胞中Fra-1、p53蛋白及mRNA表达的影响。方法　取对数生长期SKOV3细胞,以每孔5×103个细胞接种于96孔板中,培养24 h后将细胞分为A组、B组、C组和D组，分别加入0、10、30、60 μmol·L-1虫草素，培养48 h。应用细胞计数试剂盒-8检测SKOV3细胞活性，Hoechst染色检测SKOV3细胞凋亡情况，Transwell实验检测细胞侵袭能力，免疫印迹法检测SKOV3细胞中Fra-1、p53蛋白的表达，实时荧光定量反转录聚合酶链反应法检测SKOV3细胞中Fra-1、p53 mRNA的表达。 结果　A组、B组、C组及D组SKOV3细胞活性、细胞凋亡率、穿膜细胞数比较差异均有统计学意义（P<0.001）；C组和D组SKOV3细胞活性、穿膜细胞数显著低于A组、B组（P＜0.05），细胞凋亡率显著高于A组、B组（P＜0.05）；D组SKOV3细胞活性、穿膜细胞数显著低于C组（P＜0.05），细胞凋亡率显著高于C组（P＜0.05）。A组与B组SKOV3细胞中Fra-1、p53蛋白及mRNA相对表达量比较差异均无统计学意义(P＞0.05)；C组和D组SKOV3细胞中Fra-1蛋白及mRNA相对表达量显著低于A组、B组，p53蛋白及mRNA相对表达量显著高于A组、B组（P＜0.05）；D组SKOV3细胞中Fra-1蛋白及mRNA相对表达量显著低于C组，p53蛋白及mRNA相对表达量显著高于C组（P＜0.05）。结论　虫草素可抑制SKOV3细胞活性、降低其侵袭能力、促进其凋亡，且呈现浓度依赖性，其机制可能与升高p53表达和降低Fra-1表达有关。
Abstract:
Objective　To investigate the effect of cordycepin on the expression of Fra-1,p53 protein and mRNA in ovarian cancer cells.Methods　SKOV3 cells in logarithmic growth phase were taken and inoculated into 96 well plates with 5×103 cells per well.After 24 hours of culture,the cells were divided into group A,group B,group C and group D,and the cells in the four groups were added with 0,10,30 and 60 μmol·L-1 cordycepin respectively.After 48 hours of culture,the viability of SKOV3 cells was detected by cell counting kit-8,the apoptosis of SKOV3 cells was detected by Hoechst staining,the ability of invasions was detected by Transwell test.The expression levels of Fra-1,p53 protein were detected by Western blot,the expression levels of Fra-1,p53 mRNA were detected by real-time reverse transcription polymerase chain reaction.Results　There were significant differences in activity,apoptosis rates and number of transmembrane cells of SKOV3 cell among the group A,group B,group C and group D (P<0.001).The activity and the number of transmembrane cells of SKOV3 cells in group C and group D were significantly lower than those in the group A and group B (P<0.05),and the apoptosis rate of SKOV3 cells in the group C and group D was significantly higher than that in the group A and group B (P<0.05).The cell viability and number of transmembrane cells of SKOV3 in the group D were significantly lower than those in the group C (P<0.05),and the apoptosis rate of SKOV3 cells in group D was significantly higher than that the in the group C (P<0.05).There was no significant difference in the relative expression levels of Fra-1 and p53 protein and mRNA in SKOV3 cells between group A and group B (P>0.05).The relative expression levels of Fra-1 protein and mRNA of SKOV3 cells in the group C and group D were significantly lower than those in the group A and group B,and the relative expression levels of p53 protein and mRNA were significantly higher than those in the group A and group B (P<0.05).The relative expression levels of Fra-1 protein and mRNA of SKOV3 cells in the group D were significantly lower than those in the group C,and the relative expression levels of p53 protein and mRNA in SKOV3 cells in the group D were significantly higher than those in the group C (P<0.05).Conclusion　Cordycepin can inhibit the activity of SKOV3 cells,reduce its invasion ability,and promote its apoptosis in a concentration dependent manner.The mechanism may be related to increasing the expression of p53 and reducing the expression of Fra-1.

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更新日期/Last Update: 2021-08-05